FastQCFastQC Report
Wed 25 May 2016
SRR2088614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3678757
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT363470.9880239439571572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT324350.8816836773942938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT254740.692462155015947No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140420.38170501612365265No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG73660.20023067574183342No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA66470.1806860306348041No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG65010.17671729880500398No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA64420.17511349621624914No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA61330.1667139199463297No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG55070.14969730264869358No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39300.10682956226790734No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA36920.10035998572343865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112500.022.1013321
GTATTGG15950.019.253921
ATTGGAC19000.016.9421043
TTGGACC21800.016.7178924
GTATTAG7500.015.7866661
TGGACCC20900.015.6674645
TATACTG4300.015.0581395
TATACCG2109.094947E-1214.97619155
TATTGGA21000.014.7119052
GGACCCT23150.014.7041036
TAGGACG1152.2123482E-514.4782614
TACGACT2201.8189894E-1114.29545420
GTATCAA173950.014.2831272
TAGACTG4500.013.97777755
ATACGAC2009.858923E-1013.87500119
ATACTGG6500.013.6615386
TTAACGG3350.013.25373235
TAATACT7450.013.1610744
ACCGTTA5950.013.0588248
GACCGTT6550.012.9923667
Produced by FastQC (version 0.11.5)