##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2088614_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3678757 Sequences flagged as poor quality 0 Sequence length 43 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73024665668322 31.0 31.0 34.0 30.0 34.0 2 31.90871971157649 31.0 31.0 34.0 30.0 34.0 3 31.98729190321622 31.0 31.0 34.0 30.0 34.0 4 35.67036148351196 37.0 35.0 37.0 33.0 37.0 5 35.56848576842667 37.0 35.0 37.0 33.0 37.0 6 35.603248869115305 37.0 35.0 37.0 33.0 37.0 7 35.47958155431304 37.0 35.0 37.0 33.0 37.0 8 35.49267075808486 37.0 35.0 37.0 33.0 37.0 9 37.114946978014586 39.0 37.0 39.0 33.0 39.0 10 36.97243607011825 39.0 37.0 39.0 33.0 39.0 11 37.01987519153888 39.0 37.0 39.0 33.0 39.0 12 36.92032417471445 39.0 37.0 39.0 33.0 39.0 13 36.96785898062851 39.0 37.0 39.0 33.0 39.0 14 38.02204467432885 40.0 37.0 41.0 33.0 41.0 15 37.97879283681961 40.0 37.0 41.0 33.0 41.0 16 37.880697474717685 40.0 37.0 41.0 33.0 41.0 17 37.890672311326895 40.0 37.0 41.0 33.0 41.0 18 37.85824967509406 40.0 37.0 41.0 33.0 41.0 19 37.87283014344247 40.0 37.0 41.0 32.0 41.0 20 37.75549839252769 40.0 37.0 41.0 32.0 41.0 21 37.65183321431668 40.0 37.0 41.0 32.0 41.0 22 37.50872781213872 39.0 36.0 41.0 32.0 41.0 23 37.3810246232627 39.0 36.0 41.0 31.0 41.0 24 37.23976250673801 39.0 36.0 41.0 31.0 41.0 25 37.04673779757674 39.0 36.0 41.0 31.0 41.0 26 37.20827605628749 39.0 36.0 41.0 31.0 41.0 27 37.13541285820183 39.0 36.0 41.0 31.0 41.0 28 37.01875660719096 39.0 36.0 41.0 31.0 41.0 29 36.87764046388495 39.0 35.0 41.0 30.0 41.0 30 36.76832745408299 39.0 35.0 41.0 30.0 41.0 31 36.59815149519253 39.0 35.0 40.0 30.0 41.0 32 36.4780215165068 39.0 35.0 40.0 30.0 41.0 33 36.27996630383578 38.0 35.0 40.0 30.0 41.0 34 36.14722472835254 38.0 35.0 40.0 30.0 41.0 35 35.96949377194525 38.0 35.0 40.0 29.0 41.0 36 35.82864592578417 38.0 35.0 40.0 28.0 41.0 37 35.66813953734916 38.0 35.0 40.0 27.0 41.0 38 35.58435498729598 38.0 35.0 40.0 26.0 41.0 39 35.454859073322865 38.0 34.0 40.0 26.0 41.0 40 35.278062128050315 38.0 34.0 40.0 25.0 41.0 41 35.15215737272128 38.0 34.0 40.0 24.0 41.0 42 34.94900750443696 38.0 34.0 40.0 24.0 41.0 43 34.592121197458816 38.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 2.0 11 1.0 12 1.0 13 9.0 14 10.0 15 24.0 16 48.0 17 129.0 18 277.0 19 618.0 20 1299.0 21 2694.0 22 4836.0 23 8205.0 24 12997.0 25 20176.0 26 29234.0 27 41509.0 28 55772.0 29 73580.0 30 93561.0 31 115514.0 32 142728.0 33 176834.0 34 220099.0 35 273814.0 36 356668.0 37 505175.0 38 704958.0 39 837984.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.01883951562987 20.685709874286342 13.420076400806034 23.87537420927775 2 19.28678627047125 22.748716482224836 34.49787523340085 23.466622013903066 3 20.179914030744627 24.077453335460863 31.68844802741796 24.054184606376555 4 14.23075783477952 15.993527161484163 35.5975673304869 34.17814767324941 5 13.566321450424695 37.72168153536643 34.50043044430496 14.21156656990391 6 33.345937228254 36.38650228868066 13.796589445837276 16.470971037228065 7 26.966880389218424 32.07189276160399 21.332749078017386 19.628477771160206 8 27.06922474085676 34.250509071406455 19.045617854074077 19.634648333662703 9 25.004587147234787 14.317390357667007 21.225104022907736 39.452918472190476 10 16.737881844329483 27.048456856487125 33.34938948128403 22.86427181789936 11 33.91197080970556 22.114725163961634 22.90493772760745 21.06836629872536 12 21.135426993411087 26.54344932269242 30.637495219173218 21.683628464723277 13 31.48090509919519 20.45280511868547 25.75842873013901 22.30786105198033 14 20.942916316571058 22.377042028054582 27.140444449035368 29.539597206338993 15 23.634151426691137 28.774556188408205 24.7111456396821 22.88014674521856 16 22.604809178752497 27.650616770827757 26.09220995026309 23.652364100156657 17 22.334038372200173 26.34120709794096 26.820689705789214 24.50406482406965 18 23.348810481366396 25.215718243961206 28.675664089799895 22.7598071848725 19 23.380940899330945 24.748984507538825 29.59390902959886 22.27616556353138 20 24.112465161466222 24.456820605438196 29.305577943854406 22.125136289241176 21 23.18247712474621 25.15208261921078 29.548812275450647 22.11662798059236 22 22.274235563805927 25.54229594398325 29.31914230812201 22.86432618408881 23 22.879113787619023 25.652876773323165 29.94139052946416 21.526618909593648 24 22.911380121057192 25.776451121941463 28.13134980103334 23.180818955968007 25 22.987519969380962 25.699142400544535 28.83906167218982 22.474275957884686 26 22.65134663692111 26.233969789252185 28.943200108079985 22.171483465746718 27 21.94099800557634 26.279718937673785 28.764661541928426 23.014621514821446 28 22.340263300892122 26.0163691159813 28.83666955985405 22.806698023272535 29 22.341214709207485 26.108139243771745 28.474155808606007 23.076490238414767 30 22.39106850493251 26.220785988310723 29.644741416733964 21.7434040900228 31 22.853208298346424 25.843022520922148 29.422220603317918 21.88154857741351 32 22.44883258122241 25.798469428668437 28.756370698037408 22.996327292071754 33 22.28114006986599 25.688595359791368 29.281357806454732 22.74890676388791 34 21.74416521667509 26.02748700172368 29.6303343765299 22.59801340507133 35 21.601263687707558 27.36764075474406 28.93034250427522 22.100753053273156 36 22.991760532158008 25.671986488914598 28.880760539497448 22.45549243942995 37 22.21543852991649 25.940800112646745 28.978973060737633 22.86478829669913 38 22.062452072806114 24.940625325347664 30.1655423285637 22.831380273282527 39 21.58555185895671 24.84110801556069 30.695966055925954 22.877374069556648 40 20.85416351229505 25.357885829371167 30.52479410844478 23.263156549888997 41 21.772027888767866 24.570065378061123 30.423808911542675 23.23409782162834 42 20.64189072559019 26.070001361873047 30.053194598066685 23.234913314470077 43 20.841169993016663 25.93095983235642 29.5484317121245 23.679438462502418 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 183.0 1 406.5 2 630.0 3 1755.5 4 2881.0 5 2881.0 6 4394.5 7 5908.0 8 6397.5 9 6887.0 10 10263.0 11 13639.0 12 13639.0 13 25230.0 14 36821.0 15 56310.5 16 75800.0 17 70071.0 18 64342.0 19 64342.0 20 69919.5 21 75497.0 22 61964.0 23 48431.0 24 54437.0 25 60443.0 26 60443.0 27 66413.0 28 72383.0 29 79561.5 30 86740.0 31 96147.5 32 105555.0 33 105555.0 34 117327.0 35 129099.0 36 141565.0 37 154031.0 38 163851.5 39 173672.0 40 173672.0 41 179704.0 42 185736.0 43 198118.5 44 210501.0 45 225161.5 46 239822.0 47 239822.0 48 293917.0 49 348012.0 50 332943.0 51 317874.0 52 300110.0 53 282346.0 54 282346.0 55 258665.0 56 234984.0 57 208974.5 58 182965.0 59 165802.5 60 148640.0 61 148640.0 62 133319.5 63 117999.0 64 103934.5 65 89870.0 66 78861.5 67 67853.0 68 67853.0 69 58451.0 70 49049.0 71 42311.5 72 35574.0 73 29460.5 74 23347.0 75 23347.0 76 18732.0 77 14117.0 78 10958.5 79 7800.0 80 6164.0 81 4528.0 82 4528.0 83 3399.5 84 2271.0 85 1724.0 86 1177.0 87 919.5 88 662.0 89 662.0 90 540.5 91 419.0 92 288.5 93 158.0 94 113.0 95 68.0 96 68.0 97 49.0 98 30.0 99 21.5 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 3678757.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.28337275273379 #Duplication Level Percentage of deduplicated Percentage of total 1 78.08263007391393 37.70092733372608 2 12.056949323952397 11.643003569384305 3 4.0355780557774255 5.845539586195623 4 1.7857183250307933 3.4488201407539694 5 0.9838274275404701 2.375125320414986 6 0.5881370813859514 1.7038345158157695 7 0.39101535192209824 1.3215677992227237 8 0.2805026440350336 1.0834890976056741 9 0.21904215433468438 0.9518484587673067 >10 1.338244066379723 12.35497290281719 >50 0.137865844967551 4.620422660506301 >100 0.09068492386461453 8.144756655028063 >500 0.005916645543778981 1.9055650951332117 >1k 0.0033245913006467185 2.903552402443053 >5k 3.3809403057424257E-4 1.0500890978700532 >10k+ 2.253960203828284E-4 2.946485364315759 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 36347 0.9880239439571572 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 32435 0.8816836773942938 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 25474 0.692462155015947 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14042 0.38170501612365265 No Hit GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG 7366 0.20023067574183342 No Hit ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA 6647 0.1806860306348041 No Hit TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG 6501 0.17671729880500398 No Hit ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA 6442 0.17511349621624914 No Hit GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA 6133 0.1667139199463297 No Hit GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG 5507 0.14969730264869358 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3930 0.10682956226790734 No Hit GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA 3692 0.10035998572343865 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.7183094724658354E-5 0.0 0.0 0.0 3 0.0 2.7183094724658354E-5 0.0 0.0 0.0 4 0.0 2.7183094724658354E-5 0.0 0.0 0.0 5 0.0 2.7183094724658354E-5 0.0 0.0 0.0 6 0.0 2.7183094724658354E-5 0.0 0.0 0.0 7 0.0 2.7183094724658354E-5 0.0 0.0 0.0 8 2.7183094724658354E-5 2.7183094724658354E-5 0.0 0.0 0.0 9 5.436618944931671E-5 2.7183094724658354E-5 0.0 1.6309856834795014E-4 0.0 10 5.436618944931671E-5 2.7183094724658354E-5 0.0 3.261971366959003E-4 0.0 11 5.436618944931671E-5 2.7183094724658354E-5 0.0 6.523942733918006E-4 2.7183094724658354E-5 12 5.436618944931671E-5 2.7183094724658354E-5 0.0 0.001060140694261676 2.7183094724658354E-5 13 8.154928417397507E-5 2.7183094724658354E-5 0.0 0.001141689978435651 2.7183094724658354E-5 14 1.0873237889863342E-4 2.7183094724658354E-5 0.0 0.0012776054520589428 2.7183094724658354E-5 15 1.3591547362329178E-4 2.7183094724658354E-5 0.0 0.0016581687782041598 2.7183094724658354E-5 16 1.3591547362329178E-4 2.7183094724658354E-5 0.0 0.00214746448324801 2.7183094724658354E-5 17 1.3591547362329178E-4 2.7183094724658354E-5 0.0 0.0029357742302631024 2.7183094724658354E-5 18 1.6309856834795014E-4 2.7183094724658354E-5 0.0 0.003370703745857636 2.7183094724658354E-5 19 2.446478525219252E-4 2.7183094724658354E-5 0.0 0.00429492896649602 2.7183094724658354E-5 20 2.446478525219252E-4 5.436618944931671E-5 0.0 0.0068501398706139055 2.7183094724658354E-5 21 2.446478525219252E-4 5.436618944931671E-5 0.0 0.011797463110501727 8.154928417397507E-5 22 2.7183094724658356E-4 5.436618944931671E-5 0.0 0.019925208433174576 8.154928417397507E-5 23 2.7183094724658356E-4 5.436618944931671E-5 0.0 0.02528027809393227 8.154928417397507E-5 24 2.7183094724658356E-4 5.436618944931671E-5 0.0 0.034006051500547606 8.154928417397507E-5 25 2.7183094724658356E-4 5.436618944931671E-5 0.0 0.03971450139272586 8.154928417397507E-5 26 2.7183094724658356E-4 5.436618944931671E-5 0.0 0.04985379572502342 8.154928417397507E-5 27 2.7183094724658356E-4 5.436618944931671E-5 0.0 0.10704702702570461 1.3591547362329178E-4 28 2.990140419712419E-4 5.436618944931671E-5 0.0 0.22502165813072186 1.3591547362329178E-4 29 2.990140419712419E-4 5.436618944931671E-5 0.0 0.3763771295576196 1.3591547362329178E-4 30 3.5338023142055864E-4 5.436618944931671E-5 0.0 0.5871820291473452 1.3591547362329178E-4 31 3.5338023142055864E-4 5.436618944931671E-5 0.0 0.8942694502518106 1.3591547362329178E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11250 0.0 22.101332 1 GTATTGG 1595 0.0 19.25392 1 ATTGGAC 1900 0.0 16.942104 3 TTGGACC 2180 0.0 16.717892 4 GTATTAG 750 0.0 15.786666 1 TGGACCC 2090 0.0 15.667464 5 TATACTG 430 0.0 15.058139 5 TATACCG 210 9.094947E-12 14.9761915 5 TATTGGA 2100 0.0 14.711905 2 GGACCCT 2315 0.0 14.704103 6 TAGGACG 115 2.2123482E-5 14.478261 4 TACGACT 220 1.8189894E-11 14.295454 20 GTATCAA 17395 0.0 14.283127 2 TAGACTG 450 0.0 13.9777775 5 ATACGAC 200 9.858923E-10 13.875001 19 ATACTGG 650 0.0 13.661538 6 TTAACGG 335 0.0 13.253732 35 TAATACT 745 0.0 13.161074 4 ACCGTTA 595 0.0 13.058824 8 GACCGTT 655 0.0 12.992366 7 >>END_MODULE