FastQCFastQC Report
Wed 25 May 2016
SRR2088613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2961774
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT365531.2341589871475676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT318341.0748288019274934No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT253090.8545216481743713No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135690.4581375891610906No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG68640.23175299668374427No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG68310.23063879958430317No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA65400.22081360698014096No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA63010.21274411889631012No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA61280.20690302501136143No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG55440.18718511270610114No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39120.13208300160646963No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA36270.12246039029311488No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA35770.12077221286971929No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA35510.11989436060955361No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34300.11580897124493632No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32750.11057562123241005No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC32640.11020422219926301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113450.022.1119421
TATACCG2100.018.5000025
ACCGTTA6550.018.358788
GACCGTT7050.017.0567387
GGACCGT8600.016.9941866
GTATTAG4800.016.18751
CGTTATT4350.016.16092110
TGTTACG4350.016.16092116
GTATTGG14500.015.3103441
GTATCAA169000.014.8218932
ATTGGAC15400.014.7759733
TTGGACC20000.014.52254
CTAATAC5100.014.5098043
ATACACA11100.014.537
CCGTTAT8150.014.3006149
CGCCGTT7900.014.2848125
CCGTTTA7950.014.19496927
TAGTACT5400.014.0462974
CGTTTAC8100.013.93209828
AGTTCCG3900.013.75640932
Produced by FastQC (version 0.11.5)