##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088613_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2961774
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69987615530422	31.0	31.0	34.0	30.0	34.0
2	31.875738999667092	31.0	31.0	34.0	30.0	34.0
3	31.948089557137042	31.0	31.0	34.0	30.0	34.0
4	35.64053840704929	37.0	35.0	37.0	33.0	37.0
5	35.550446793036876	37.0	35.0	37.0	33.0	37.0
6	35.58103116578105	37.0	35.0	37.0	33.0	37.0
7	35.45076464308215	37.0	35.0	37.0	33.0	37.0
8	35.47863071253917	37.0	35.0	37.0	33.0	37.0
9	37.0822047867258	39.0	37.0	39.0	33.0	39.0
10	36.94501065915225	39.0	37.0	39.0	33.0	39.0
11	36.99028183784448	39.0	37.0	39.0	33.0	39.0
12	36.89390513928477	39.0	37.0	39.0	33.0	39.0
13	36.94025877734088	39.0	37.0	39.0	33.0	39.0
14	37.95965998756151	40.0	37.0	41.0	33.0	41.0
15	37.92635832443664	40.0	37.0	41.0	33.0	41.0
16	37.821510351566324	40.0	37.0	41.0	32.0	41.0
17	37.86421381239757	40.0	37.0	41.0	33.0	41.0
18	37.84242045476799	40.0	37.0	41.0	32.0	41.0
19	37.86502920209307	40.0	37.0	41.0	32.0	41.0
20	37.75265769771765	40.0	37.0	41.0	32.0	41.0
21	37.65281517090771	40.0	37.0	41.0	32.0	41.0
22	37.50426129745213	39.0	36.0	41.0	32.0	41.0
23	37.37232145329117	39.0	36.0	41.0	31.0	41.0
24	37.23240868479499	39.0	36.0	41.0	31.0	41.0
25	37.03613982700908	39.0	36.0	41.0	31.0	41.0
26	37.18383846978196	39.0	36.0	41.0	31.0	41.0
27	37.10355179024463	39.0	36.0	41.0	31.0	41.0
28	36.97561967928681	39.0	36.0	41.0	31.0	41.0
29	36.82272718985311	39.0	35.0	41.0	30.0	41.0
30	36.69202984427576	39.0	35.0	40.0	30.0	41.0
31	36.499880477038424	39.0	35.0	40.0	30.0	41.0
32	36.37725667117073	38.0	35.0	40.0	30.0	41.0
33	36.17248513897414	38.0	35.0	40.0	30.0	41.0
34	36.02274920368671	38.0	35.0	40.0	29.0	41.0
35	35.840688384731585	38.0	35.0	40.0	28.0	41.0
36	35.68414875679238	38.0	35.0	40.0	27.0	41.0
37	35.51125946814308	38.0	35.0	40.0	27.0	41.0
38	35.40835053586128	38.0	34.0	40.0	26.0	41.0
39	35.25045057455431	38.0	34.0	40.0	25.0	41.0
40	35.058138804648834	38.0	34.0	40.0	24.0	41.0
41	34.908575401094076	38.0	34.0	40.0	24.0	41.0
42	34.67636997286086	38.0	33.0	40.0	23.0	41.0
43	34.30327162031944	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	2.0
12	3.0
13	5.0
14	10.0
15	14.0
16	29.0
17	93.0
18	205.0
19	459.0
20	970.0
21	2055.0
22	3780.0
23	6461.0
24	10776.0
25	16732.0
26	24686.0
27	34439.0
28	46477.0
29	61110.0
30	77655.0
31	96088.0
32	118264.0
33	146992.0
34	181437.0
35	225024.0
36	291220.0
37	413703.0
38	555642.0
39	647441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77341012514797	21.136487794139594	13.928915575597598	23.161186505114838
2	19.090653101823435	23.14109719377643	34.8811556857478	22.887094018652334
3	20.084516914524876	24.424483434590215	32.625480539703574	22.86551911118134
4	13.904031840376746	16.160888710617353	35.98637168129641	33.948707767709486
5	13.124465269801139	38.1732704791115	34.60399071637471	14.098273534712641
6	32.49289108487008	36.87614247407128	13.673967021116399	16.956999419942235
7	25.903968364905626	32.93137828882285	21.51723933021223	19.647414016059294
8	27.14336070206572	34.275775261718145	18.966133135073775	19.614730901142355
9	24.610891985681555	14.365782129223902	21.914973931164226	39.10835195393032
10	16.888358125906976	27.228647425495666	33.20030495236976	22.6826894962276
11	33.23940314149561	22.49800288610812	23.293168216075905	20.969425756320366
12	20.927862828156368	27.05209783055696	30.868796876466604	21.151242464820072
13	31.47691214792216	20.71609785216563	26.334960061098517	21.472029938813698
14	20.504096531335612	23.08845982171496	27.732838494766987	28.67460515218244
15	23.207780202000556	29.23690328836704	25.3671617078143	22.1881548018181
16	21.953802011902326	28.09454738950372	26.77638469376799	23.17526590482596
17	21.815168881893083	26.563471757129342	27.257481495887266	24.363877865090313
18	22.951548632677575	25.17660024026141	29.585680744040566	22.286170383020444
19	22.90529257127654	24.924319006109176	30.4934812716973	21.676907150916986
20	23.829164548004	24.436435730747856	30.249708451758977	21.484691269489165
21	22.749845194130273	25.16738279152967	30.70710324285378	21.37566877148628
22	21.590101067805982	25.679744639530227	30.418627484743944	22.311526807919847
23	22.326787931827344	25.76631437780195	31.234456106374086	20.67244158399662
24	22.40478172878822	25.86628824481544	29.059408314071227	22.669521712325114
25	22.308353034363865	25.913219577185835	30.0106287650577	21.7677986233926
26	22.059414391509954	26.555773668078658	29.97683145304132	21.40798048737007
27	21.255909465070598	26.69092915259571	29.605770055378972	22.447391326954723
28	21.512681251169063	26.461843476240926	29.740925539896025	22.284549732693986
29	21.755508691750283	26.45080279589192	29.25692507260851	22.536763439749286
30	21.651922125050728	26.558542279053025	30.82196683474161	20.96756876115463
31	22.186838023427853	26.097028335045145	30.670807428250775	21.045326213276233
32	21.867806253954555	26.04567397782545	29.655841397756884	22.43067837046311
33	21.560017746121073	26.098378876983862	30.253186097251174	22.088417279643892
34	20.925533143312084	26.565565097134353	30.556821688623103	21.952080070930464
35	20.659510144933407	28.247057337933278	29.794575818411534	21.298856698721778
36	22.460660401502615	26.150678613560657	29.688153113640674	21.700507871296054
37	21.588851816512673	26.565294988746608	29.694635714946514	22.151217479794205
38	21.356524839504974	25.3778985162271	30.96441524572773	22.3011613985402
39	20.832075641152905	25.3254974890049	31.630570056999623	22.211856812842573
40	20.065946962867525	25.935402228529252	31.23519890444038	22.763451904162842
41	21.098098639531578	24.891568364095303	31.389059394808648	22.621273601564468
42	19.958578878739566	26.639237159891337	30.786278764011026	22.615905197358067
43	20.175610968291302	26.512218690555052	30.121271913387044	23.1908984277666
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	155.0
1	352.0
2	549.0
3	1573.5
4	2598.0
5	2598.0
6	3953.0
7	5308.0
8	5916.0
9	6524.0
10	9694.5
11	12865.0
12	12865.0
13	23835.5
14	34806.0
15	54747.0
16	74688.0
17	68194.0
18	61700.0
19	61700.0
20	67037.5
21	72375.0
22	57994.0
23	43613.0
24	48922.0
25	54231.0
26	54231.0
27	57462.5
28	60694.0
29	65108.5
30	69523.0
31	75907.0
32	82291.0
33	82291.0
34	91646.0
35	101001.0
36	111535.0
37	122069.0
38	130223.5
39	138378.0
40	138378.0
41	143994.0
42	149610.0
43	161350.0
44	173090.0
45	183984.0
46	194878.0
47	194878.0
48	247542.5
49	300207.0
50	285834.0
51	271461.0
52	252742.5
53	234024.0
54	234024.0
55	209935.5
56	185847.0
57	161753.0
58	137659.0
59	121928.0
60	106197.0
61	106197.0
62	93690.5
63	81184.0
64	70798.0
65	60412.0
66	51657.0
67	42902.0
68	42902.0
69	36800.0
70	30698.0
71	25336.5
72	19975.0
73	16638.5
74	13302.0
75	13302.0
76	10478.5
77	7655.0
78	6017.5
79	4380.0
80	3348.5
81	2317.0
82	2317.0
83	1785.0
84	1253.0
85	940.5
86	628.0
87	488.0
88	348.0
89	348.0
90	295.0
91	242.0
92	164.0
93	86.0
94	59.0
95	32.0
96	32.0
97	23.0
98	14.0
99	9.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2961774.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.84688779983992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26228014757439	35.88081976885482
2	12.128839821251415	11.12139116854288
3	4.043299943969094	5.5611815661675035
4	1.7428656305653798	3.1961986005891294
5	0.921130822398144	2.1115490731730993
6	0.5553456865354429	1.527652282842931
7	0.34868901462468455	1.119041429137426
8	0.25767714219500265	0.9450956021438146
9	0.19420348723373887	0.8013262940588582
>10	1.2604035700966174	11.293506099894582
>50	0.15687923795314512	5.055942035487566
>100	0.1150968430978529	10.264050832087872
>500	0.008047017350239731	2.5423725429777866
>1k	0.004503376682238916	3.6560694057981893
>5k	4.429550834989098E-4	1.2932205730224335
>10k+	2.9530338899927324E-4	3.630582725221193
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36553	1.2341589871475676	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31834	1.0748288019274934	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25309	0.8545216481743713	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13569	0.4581375891610906	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6864	0.23175299668374427	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6831	0.23063879958430317	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6540	0.22081360698014096	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6301	0.21274411889631012	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6128	0.20690302501136143	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5544	0.18718511270610114	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3912	0.13208300160646963	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3627	0.12246039029311488	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3577	0.12077221286971929	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3551	0.11989436060955361	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3430	0.11580897124493632	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3275	0.11057562123241005	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3264	0.11020422219926301	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.3763548467911463E-5	0.0	0.0	0.0	0.0
6	3.3763548467911463E-5	0.0	0.0	0.0	0.0
7	6.752709693582293E-5	0.0	0.0	0.0	0.0
8	6.752709693582293E-5	0.0	0.0	0.0	0.0
9	1.0129064540373438E-4	0.0	0.0	1.6881774233955732E-4	0.0
10	1.3505419387164585E-4	0.0	0.0	3.3763548467911463E-4	0.0
11	2.0258129080746875E-4	0.0	0.0	4.3892613008284897E-4	0.0
12	2.0258129080746875E-4	0.0	0.0	8.10325163229875E-4	0.0
13	2.3634483927538022E-4	0.0	0.0	9.116158086336095E-4	0.0
14	2.701083877432917E-4	0.0	0.0	0.0011479606479089896	0.0
15	2.701083877432917E-4	0.0	0.0	0.0014518325841201929	0.0
16	3.0387193621120317E-4	0.0	0.0	0.0021608671019463337	0.0
17	3.0387193621120317E-4	0.0	0.0	0.002869901619772474	0.0
18	3.0387193621120317E-4	0.0	0.0	0.003308827749855323	0.0
19	3.3763548467911463E-4	0.0	0.0	0.004254207106956844	0.0
20	3.3763548467911463E-4	0.0	0.0	0.0070228180813255835	0.0
21	3.3763548467911463E-4	0.0	0.0	0.012661330675466797	0.0
22	3.3763548467911463E-4	0.0	0.0	0.02103469069550884	0.0
23	3.3763548467911463E-4	0.0	0.0	0.025829114577952266	0.0
24	3.713990331470261E-4	0.0	0.0	0.034134947501058485	3.3763548467911463E-5
25	3.713990331470261E-4	0.0	0.0	0.0397734600951997	3.3763548467911463E-5
26	3.713990331470261E-4	0.0	0.0	0.04990252463557314	3.3763548467911463E-5
27	4.051625816149375E-4	0.0	0.0	0.10655775896472858	3.3763548467911463E-5
28	4.051625816149375E-4	0.0	0.0	0.22824158764308147	3.3763548467911463E-5
29	4.051625816149375E-4	0.0	0.0	0.38433047221023614	3.3763548467911463E-5
30	4.051625816149375E-4	0.0	0.0	0.6003158917594658	3.3763548467911463E-5
31	4.051625816149375E-4	0.0	0.0	0.9004400740907308	3.3763548467911463E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11345	0.0	22.111942	1
TATACCG	210	0.0	18.500002	5
ACCGTTA	655	0.0	18.35878	8
GACCGTT	705	0.0	17.056738	7
GGACCGT	860	0.0	16.994186	6
GTATTAG	480	0.0	16.1875	1
CGTTATT	435	0.0	16.160921	10
TGTTACG	435	0.0	16.160921	16
GTATTGG	1450	0.0	15.310344	1
GTATCAA	16900	0.0	14.821893	2
ATTGGAC	1540	0.0	14.775973	3
TTGGACC	2000	0.0	14.5225	4
CTAATAC	510	0.0	14.509804	3
ATACACA	1110	0.0	14.5	37
CCGTTAT	815	0.0	14.300614	9
CGCCGTT	790	0.0	14.28481	25
CCGTTTA	795	0.0	14.194969	27
TAGTACT	540	0.0	14.046297	4
CGTTTAC	810	0.0	13.932098	28
AGTTCCG	390	0.0	13.756409	32
>>END_MODULE