FastQCFastQC Report
Wed 25 May 2016
SRR2088612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126338
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT359661.150419436414105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT324641.0384033972014544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT256450.8202887851537486No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138310.4424025809109572No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG77340.2473820808882469No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA76570.2449191354229773No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA74010.2367306414085745No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG69420.22204892753118827No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA64100.20503221340750744No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG56720.1814263205066119No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA44040.14086768609152306No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA39710.1270176161374746No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38090.12183583476898531No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG35880.11476686142061415No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35270.11281569683124473No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA35240.11271973791701345No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35230.1126877516122697No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34870.1115362446414943No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC34140.10920124439519976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119050.020.8542611
ACCGTTA5450.017.6513778
GTATTGG15800.016.9778481
TTGGACC23700.016.3143464
CTCTAAT6200.016.1129041
ATTAGAG5950.015.8571423
GTATTAG7400.015.5000011
ATTGGAC19850.015.3778343
TGGACCC22500.015.3755565
CCGTTTA5850.015.17948727
TCGGGTA3550.015.11267725
TTAACGG4900.015.1020435
TAGGGGT2700.015.0740754
GGACCGT7600.014.8486846
GGACCCT24500.014.6489796
TATACCG2301.8189894E-1214.4782615
TAATACT7200.014.1319454
GTATCAA176300.014.1137272
ATACTGG7150.013.9720276
TCTAATA7150.013.9720272
Produced by FastQC (version 0.11.5)