##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088612_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3126338
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.726624568424782	31.0	31.0	34.0	30.0	34.0
2	31.89919835923051	31.0	31.0	34.0	30.0	34.0
3	31.97525123643061	31.0	31.0	34.0	30.0	34.0
4	35.65374537238136	37.0	35.0	37.0	33.0	37.0
5	35.56334567791455	37.0	35.0	37.0	33.0	37.0
6	35.60271250261488	37.0	35.0	37.0	33.0	37.0
7	35.477321709936675	37.0	35.0	37.0	33.0	37.0
8	35.500307388388585	37.0	35.0	37.0	33.0	37.0
9	37.11269031051665	39.0	37.0	39.0	33.0	39.0
10	36.98614641155243	39.0	37.0	39.0	33.0	39.0
11	37.02476283754348	39.0	37.0	39.0	33.0	39.0
12	36.928985925386186	39.0	37.0	39.0	33.0	39.0
13	36.975475140563816	39.0	37.0	39.0	33.0	39.0
14	38.01372020555679	40.0	37.0	41.0	33.0	41.0
15	37.97638323175549	40.0	37.0	41.0	33.0	41.0
16	37.87340876130476	40.0	37.0	41.0	32.0	41.0
17	37.90693520662193	40.0	37.0	41.0	33.0	41.0
18	37.88515733103714	40.0	37.0	41.0	33.0	41.0
19	37.90205089788756	40.0	37.0	41.0	32.0	41.0
20	37.793517207672366	40.0	37.0	41.0	32.0	41.0
21	37.69231349905225	40.0	37.0	41.0	32.0	41.0
22	37.54756363515397	39.0	36.0	41.0	32.0	41.0
23	37.42940814460881	39.0	36.0	41.0	32.0	41.0
24	37.29049770050455	39.0	36.0	41.0	31.0	41.0
25	37.09495550385147	39.0	36.0	41.0	31.0	41.0
26	37.253382711658176	39.0	36.0	41.0	31.0	41.0
27	37.18153187531227	39.0	36.0	41.0	31.0	41.0
28	37.06605779669377	39.0	36.0	41.0	31.0	41.0
29	36.917978798197765	39.0	35.0	41.0	31.0	41.0
30	36.786859578203	39.0	35.0	41.0	30.0	41.0
31	36.60700794347892	39.0	35.0	40.0	30.0	41.0
32	36.48791397475257	39.0	35.0	40.0	30.0	41.0
33	36.290133056630474	38.0	35.0	40.0	30.0	41.0
34	36.1469185353599	38.0	35.0	40.0	30.0	41.0
35	35.9463858354407	38.0	35.0	40.0	29.0	41.0
36	35.7880072468172	38.0	35.0	40.0	28.0	41.0
37	35.62272921226048	38.0	35.0	40.0	27.0	41.0
38	35.52751781797106	38.0	35.0	40.0	26.0	41.0
39	35.38864031976069	38.0	34.0	40.0	25.0	41.0
40	35.197706070169	38.0	34.0	40.0	25.0	41.0
41	35.057029342316795	38.0	34.0	40.0	24.0	41.0
42	34.826524195400495	38.0	34.0	40.0	23.0	41.0
43	34.46898352001607	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	5.0
14	16.0
15	24.0
16	42.0
17	99.0
18	192.0
19	481.0
20	1017.0
21	2042.0
22	3916.0
23	6641.0
24	11125.0
25	17098.0
26	24905.0
27	35151.0
28	48352.0
29	62686.0
30	79638.0
31	97991.0
32	121938.0
33	151103.0
34	186854.0
35	232224.0
36	304630.0
37	433220.0
38	594376.0
39	710571.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.894670377930986	21.1053315412473	13.822593718273584	23.17740436254813
2	18.910079460378245	23.029979483984135	35.29148159923847	22.76845945639915
3	19.933993061530774	24.74297404823151	32.27056703401872	23.052465856219
4	13.858674270024546	16.083993477352738	36.16531545853328	33.89201679408944
5	13.11537652038903	38.11072251304881	34.739525924580136	14.034375041982026
6	32.46837034255413	36.74618675267997	13.771127753940874	17.014315150825023
7	26.04196987018038	32.75666290720965	21.679293793569347	19.52207342904062
8	26.807178238565378	34.55330165836196	19.074648998284893	19.564871104787773
9	24.36169729568588	14.618540925517332	22.082737055302402	38.93702472349439
10	16.82454040478029	27.05273710008323	33.37838071251413	22.744341782622353
11	32.932107788729176	22.376947086335512	23.592714543341124	21.098230581594184
12	20.957394881807407	26.987804901453394	30.90791846562976	21.146881751109444
13	31.313152960428464	20.705214855207593	26.468923065900103	21.51270911846384
14	20.49077866820542	22.884569742619	27.739387104017542	28.885264485158036
15	23.113079903708428	29.14377780009711	25.499354196507223	22.243788099687237
16	21.992599648534483	28.07070124855342	26.69369082933451	23.243008273577583
17	21.7459212663506	26.528513551637733	27.16529690647652	24.560268275535147
18	23.00352680996105	25.259169034186325	29.477011122917613	22.260293032935017
19	22.874206179882023	24.77963035346786	30.46462026818597	21.881543198464147
20	23.815115320224493	24.296925028579764	30.304176963591267	21.583782687604476
21	22.65945012983241	25.25625828045464	30.681903236310344	21.402388353402607
22	21.541144943381042	25.69402284717775	30.224371133255584	22.54046107618562
23	22.260932759029895	25.813299777567238	31.198705962055286	20.72706150134758
24	22.41542661094226	25.921989241086536	28.906343459984175	22.75624068798703
25	22.355612221071425	25.931265269462227	29.800584581705497	21.91253792776085
26	22.148980692426733	26.632724932492902	29.875080685453714	21.34321368962665
27	21.30044800018424	26.72887576455265	29.44691201015373	22.523764225109378
28	21.434598562279575	26.411955457151464	29.797449923840606	22.35599605672835
29	21.936239779575978	26.302274418185114	29.176691707678444	22.584794094560472
30	21.655815845887425	26.44825991303563	30.79084859026759	21.10507565080935
31	22.284570638235532	25.996357399615782	30.65535460337302	21.063717358775666
32	21.81386017762635	25.859743892055175	29.62824237174611	22.69815355857236
33	21.547766108463	26.008991989989568	30.242283463912088	22.200958437635343
34	21.010971942253203	26.404438675536685	30.692746593618477	21.891842788591635
35	20.71266766421289	28.209521811141343	29.61333675373552	21.464473770910246
36	22.470155178358834	26.09202843710437	29.682107308934608	21.75570907560219
37	21.59453008599838	26.50516354917479	29.613784562001932	22.2865218028249
38	21.3965348596345	25.209238412481312	30.991338748401486	22.402887979482706
39	20.86124404974766	25.09984525025765	31.623164226004995	22.4157464739897
40	19.97032950371969	25.951512600365028	31.233027267045344	22.845130628869942
41	21.18750435813402	24.769202818121393	31.33563933266333	22.70765349108126
42	19.848717573083906	26.70258302205328	30.803899002603046	22.64480040225977
43	20.184285896150705	26.46949881938549	30.055675362036986	23.290539922426813
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	187.0
1	434.5
2	682.0
3	1742.0
4	2802.0
5	2802.0
6	4426.5
7	6051.0
8	6561.0
9	7071.0
10	10520.5
11	13970.0
12	13970.0
13	24911.5
14	35853.0
15	55242.5
16	74632.0
17	68628.5
18	62625.0
19	62625.0
20	67343.5
21	72062.0
22	57135.5
23	42209.0
24	47126.0
25	52043.0
26	52043.0
27	56464.5
28	60886.0
29	66831.0
30	72776.0
31	79777.0
32	86778.0
33	86778.0
34	97104.0
35	107430.0
36	119062.5
37	130695.0
38	140769.5
39	150844.0
40	150844.0
41	156754.5
42	162665.0
43	175721.5
44	188778.0
45	200826.0
46	212874.0
47	212874.0
48	269386.0
49	325898.0
50	309955.5
51	294013.0
52	273008.5
53	252004.0
54	252004.0
55	225334.0
56	198664.0
57	169290.0
58	139916.0
59	123821.0
60	107726.0
61	107726.0
62	95142.5
63	82559.0
64	70888.5
65	59218.0
66	50610.5
67	42003.0
68	42003.0
69	35850.0
70	29697.0
71	24916.0
72	20135.0
73	16671.0
74	13207.0
75	13207.0
76	10501.0
77	7795.0
78	6061.5
79	4328.0
80	3389.5
81	2451.0
82	2451.0
83	1857.0
84	1263.0
85	972.0
86	681.0
87	536.5
88	392.0
89	392.0
90	338.0
91	284.0
92	189.0
93	94.0
94	82.5
95	71.0
96	71.0
97	44.0
98	17.0
99	13.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3126338.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.772630742303086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82028593484863	36.07811843098571
2	11.610049884522867	10.628450525279675
3	3.842439403798071	5.276356799391729
4	1.6849011114660435	3.08489425649725
5	0.919957620049857	2.105444022055503
6	0.5675624557130035	1.5587296025127633
7	0.386091653917349	1.2370701495210725
8	0.2764107245462181	1.0121636822293194
9	0.21732556613363413	0.8952806600547123
>10	1.4010491989944818	12.349559394466308
>50	0.15159426515182708	4.806777304298779
>100	0.10850545116225813	9.541317781019389
>500	0.008632932754252748	2.727609261980787
>1k	0.004491932489991033	3.8882318525382584
>5k	4.2111867093665926E-4	1.3433884420865885
>10k+	2.8074578062443954E-4	3.4666078350821556
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35966	1.150419436414105	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	32464	1.0384033972014544	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25645	0.8202887851537486	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13831	0.4424025809109572	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7734	0.2473820808882469	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7657	0.2449191354229773	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7401	0.2367306414085745	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6942	0.22204892753118827	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6410	0.20503221340750744	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5672	0.1814263205066119	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4404	0.14086768609152306	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3971	0.1270176161374746	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3809	0.12183583476898531	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3588	0.11476686142061415	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3527	0.11281569683124473	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3524	0.11271973791701345	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3523	0.1126877516122697	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3487	0.1115362446414943	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3414	0.10920124439519976	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.198630474376091E-5	0.0	0.0	0.0	0.0
2	3.198630474376091E-5	0.0	0.0	0.0	0.0
3	3.198630474376091E-5	0.0	0.0	0.0	0.0
4	3.198630474376091E-5	0.0	0.0	0.0	0.0
5	3.198630474376091E-5	0.0	0.0	0.0	0.0
6	1.5993152371880455E-4	0.0	0.0	0.0	0.0
7	1.5993152371880455E-4	0.0	0.0	0.0	0.0
8	1.5993152371880455E-4	0.0	0.0	6.397260948752182E-5	0.0
9	1.9191782846256548E-4	0.0	0.0	2.5589043795008727E-4	0.0
10	2.2390413320632637E-4	0.0	0.0	2.878767426938482E-4	0.0
11	2.5589043795008727E-4	3.198630474376091E-5	0.0	5.757534853876964E-4	0.0
12	3.198630474376091E-4	3.198630474376091E-5	0.0	9.915754470565883E-4	0.0
13	3.198630474376091E-4	3.198630474376091E-5	0.0	0.0011515069707753929	0.0
14	3.8383565692513095E-4	3.198630474376091E-5	0.0	0.0015353426277005238	0.0
15	3.8383565692513095E-4	3.198630474376091E-5	0.0	0.00175924676090685	0.0
16	3.8383565692513095E-4	3.198630474376091E-5	0.0	0.002526918074757112	0.0
17	3.8383565692513095E-4	3.198630474376091E-5	0.0	0.003582466131301222	0.0
18	4.1582196166889185E-4	3.198630474376091E-5	0.0	0.004254178530920201	0.0
19	4.1582196166889185E-4	3.198630474376091E-5	0.0	0.005789521158620725	0.0
20	4.1582196166889185E-4	3.198630474376091E-5	0.0	0.009084110547228099	0.0
21	4.1582196166889185E-4	3.198630474376091E-5	0.0	0.015705275629186607	0.0
22	5.117808759001745E-4	3.198630474376091E-5	0.0	0.025812947928215056	0.0
23	5.117808759001745E-4	3.198630474376091E-5	0.0	0.03304185280030502	0.0
24	5.437671806439355E-4	3.198630474376091E-5	0.0	0.044524936203315185	0.0
25	5.437671806439355E-4	3.198630474376091E-5	0.0	0.05242555347502413	0.0
26	5.437671806439355E-4	3.198630474376091E-5	0.0	0.0637487053543155	0.0
27	5.437671806439355E-4	3.198630474376091E-5	0.0	0.12199576629270412	0.0
28	5.437671806439355E-4	3.198630474376091E-5	0.0	0.2511244785432669	0.0
29	5.437671806439355E-4	3.198630474376091E-5	0.0	0.4168455234206922	0.0
30	5.437671806439355E-4	3.198630474376091E-5	0.0	0.6510812330592534	0.0
31	5.437671806439355E-4	3.198630474376091E-5	0.0	0.997492913434184	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11905	0.0	20.854261	1
ACCGTTA	545	0.0	17.651377	8
GTATTGG	1580	0.0	16.977848	1
TTGGACC	2370	0.0	16.314346	4
CTCTAAT	620	0.0	16.112904	1
ATTAGAG	595	0.0	15.857142	3
GTATTAG	740	0.0	15.500001	1
ATTGGAC	1985	0.0	15.377834	3
TGGACCC	2250	0.0	15.375556	5
CCGTTTA	585	0.0	15.179487	27
TCGGGTA	355	0.0	15.112677	25
TTAACGG	490	0.0	15.10204	35
TAGGGGT	270	0.0	15.074075	4
GGACCGT	760	0.0	14.848684	6
GGACCCT	2450	0.0	14.648979	6
TATACCG	230	1.8189894E-12	14.478261	5
TAATACT	720	0.0	14.131945	4
GTATCAA	17630	0.0	14.113727	2
ATACTGG	715	0.0	13.972027	6
TCTAATA	715	0.0	13.972027	2
>>END_MODULE