FastQCFastQC Report
Wed 25 May 2016
SRR2088611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164221
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT125911.0814956954049102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT119911.0299590885235708No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82070.7049348877919226No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48270.4146120023603766No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG24500.2104411447654698No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA22030.18922524159931833No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22030.18922524159931833No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG21960.18862398118570273No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA20380.17505267470694996No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG15950.13700147995956094No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14480.12437501127363275No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13330.11449716162137602No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA11880.10204248162505229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38150.022.4521621
CTAACGA555.143149E-420.18181814
CGGTATA701.2192146E-418.526
TAATACT3750.017.7599984
GTATTAG3250.017.6461541
TTAACGG1800.017.47222135
TAAACGT852.7231648E-517.4117644
TAGAGTG650.001579950717.0769235
GGTAAAC2600.017.07692335
CGACAAT650.001579950717.07692321
CTCTAAT3200.016.7656251
GTTTAGG2100.016.7380941
CACATGT2200.015.97727328
TATACCG700.002592520815.85714231
AATACTG3900.015.6538475
ATTAGAG2500.015.543
GTATCAA56150.015.2546752
ATAGAAC1101.4518213E-515.1363643
TACACTT2201.8189894E-1215.1363645
GTGACAC2450.015.1020424
Produced by FastQC (version 0.11.5)