FastQCFastQC Report
Wed 25 May 2016
SRR2088610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3630973
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT393921.0848882654869645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT376051.0356728072613044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT284470.783453911665No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150630.41484748027594803No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG86450.2380904512371753No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA83030.22867148833108925No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA80540.22181382235560548No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG80070.22051940347669893No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA77280.21283551268489193No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG64890.17871242777073804No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46200.12723862171379408No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA44700.12310749763217738No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41960.11556131097642422No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA40950.11277968742813564No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40330.11107215614106743No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC38350.1056190723533334No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37590.10352596948531427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124050.023.5034261
ACGTTTA1500.020.96666726
GTATTGG18050.018.1412731
ACCGTTA8700.016.1609218
CTAATAC7600.016.065793
GTATTAG9000.015.8277781
TTAACGG6350.015.73228435
ATTGGAC21450.015.6107233
CGTTTAC8300.015.60240928
GACCGTT8900.015.5898897
GTATCAA187650.015.5374372
CCGTTTA8500.015.4529427
TTGGACC26150.015.2103254
TGGACCC26100.014.9559395
CTCTAAT7200.014.9027791
GCCGTTT8950.014.67597826
TCTAATA7750.014.561292
CGCCGTT9200.014.4782625
TAATACT8950.014.46927454
AATACTG9100.014.4340665
Produced by FastQC (version 0.11.5)