##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088610_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3630973
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.726604136136512	31.0	31.0	34.0	30.0	34.0
2	31.90213752622231	31.0	31.0	34.0	30.0	34.0
3	31.97040242381312	31.0	31.0	34.0	30.0	34.0
4	35.65896551695647	37.0	35.0	37.0	33.0	37.0
5	35.56638234434682	37.0	35.0	37.0	33.0	37.0
6	35.60095406933623	37.0	35.0	37.0	33.0	37.0
7	35.473748496615094	37.0	35.0	37.0	33.0	37.0
8	35.49712928187569	37.0	35.0	37.0	33.0	37.0
9	37.109454683358976	39.0	37.0	39.0	33.0	39.0
10	36.973641775909655	39.0	37.0	39.0	33.0	39.0
11	37.020369471213364	39.0	37.0	39.0	33.0	39.0
12	36.922301818272956	39.0	37.0	39.0	33.0	39.0
13	36.970008314575736	39.0	37.0	39.0	33.0	39.0
14	38.01251345025149	40.0	37.0	41.0	33.0	41.0
15	37.97197941157921	40.0	37.0	41.0	33.0	41.0
16	37.86536639077184	40.0	37.0	41.0	32.0	41.0
17	37.900445693206755	40.0	37.0	41.0	33.0	41.0
18	37.87762674082126	40.0	37.0	41.0	33.0	41.0
19	37.90227220086737	40.0	37.0	41.0	32.0	41.0
20	37.78224624639181	40.0	37.0	41.0	32.0	41.0
21	37.68422541285766	40.0	37.0	41.0	32.0	41.0
22	37.53920478064695	39.0	36.0	41.0	32.0	41.0
23	37.414827650880355	39.0	36.0	41.0	32.0	41.0
24	37.27620034629836	39.0	36.0	41.0	31.0	41.0
25	37.07836522056209	39.0	36.0	41.0	31.0	41.0
26	37.23295050665483	39.0	36.0	41.0	31.0	41.0
27	37.16133031008493	39.0	36.0	41.0	31.0	41.0
28	37.04060674645611	39.0	36.0	41.0	31.0	41.0
29	36.89860927084834	39.0	35.0	41.0	30.0	41.0
30	36.77378377641475	39.0	35.0	41.0	30.0	41.0
31	36.59150839182775	39.0	35.0	40.0	30.0	41.0
32	36.47710352018591	39.0	35.0	40.0	30.0	41.0
33	36.28388533872326	38.0	35.0	40.0	30.0	41.0
34	36.144244256291636	38.0	35.0	40.0	30.0	41.0
35	35.96065710210459	38.0	35.0	40.0	29.0	41.0
36	35.82008872002078	38.0	35.0	40.0	28.0	41.0
37	35.653960247019185	38.0	35.0	40.0	27.0	41.0
38	35.56394801062966	38.0	35.0	40.0	26.0	41.0
39	35.429608262027834	38.0	34.0	40.0	26.0	41.0
40	35.25008998965291	38.0	34.0	40.0	25.0	41.0
41	35.11509587099656	38.0	34.0	40.0	24.0	41.0
42	34.89792598292524	38.0	34.0	40.0	23.0	41.0
43	34.529486724357355	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	1.0
11	4.0
12	5.0
13	8.0
14	17.0
15	27.0
16	56.0
17	114.0
18	274.0
19	564.0
20	1212.0
21	2430.0
22	4393.0
23	7845.0
24	12597.0
25	20076.0
26	28678.0
27	40073.0
28	54776.0
29	72499.0
30	92110.0
31	114414.0
32	141538.0
33	176103.0
34	218954.0
35	271198.0
36	353450.0
37	501567.0
38	692784.0
39	823205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7719437737488	20.98787294755428	13.779777486640633	23.46040579205629
2	19.145749637906974	22.708265800929944	35.15170726964921	22.994277291513875
3	19.89048114651362	24.462286004329968	32.22874419611493	23.418488653041486
4	13.895696828370799	16.13427034571725	35.982751730734435	33.987281095177515
5	13.143942408825405	37.90052969272975	34.89144645250736	14.064081445937493
6	32.65653586517994	36.678212699461	13.685918347506302	16.979333087852762
7	26.26004104134071	32.563392787553084	21.630620772999414	19.545945398106788
8	26.747073029736107	34.62430042856281	18.92181517185614	19.706811369844942
9	24.545238975888832	14.456317907073393	21.726049739284758	39.27239337775302
10	17.010426681773726	26.857924859259484	33.337895930374586	22.793752528592197
11	33.38768974597167	22.258744419195626	23.26712426669105	21.08644156814165
12	21.067107907439684	26.845118374606475	30.710445932811947	21.37732778514189
13	31.3179690402545	20.682224847169064	26.21583801366741	21.783968098909025
14	20.689743492997607	22.71842836617072	27.39819326665332	29.193634874178354
15	23.294224440666454	28.94987101253576	25.351469151657145	22.40443539514064
16	22.209391256833914	27.903512364316676	26.581800525644226	23.305295853205187
17	21.980003706995344	26.490750550885394	26.890147627096102	24.639098115023163
18	23.084280714838695	25.18509501447684	29.24017887216457	22.490445398519903
19	23.18439162174987	24.586026940987992	30.1517251711869	22.077856266075237
20	23.954956426280226	24.190733448031697	30.099314977004788	21.754995148683285
21	22.93007962328555	25.00880617950065	30.420303318146402	21.640810879067402
22	21.754444332139073	25.50679941712593	30.000443407318095	22.738312843416903
23	22.51528722466402	25.690414112140193	31.001084282367287	20.793214380828502
24	22.621512195215992	25.726630299922366	28.624806628966947	23.027050875894698
25	22.60297721850314	25.65667659880699	29.666703663177884	22.07364251951199
26	22.26458307456431	26.355111976872315	29.669182337626854	21.711122610936517
27	21.491236646485667	26.460317936817486	29.224535682308844	22.823909734388
28	21.752020739344523	26.060452666544204	29.54929711677834	22.638229477332935
29	22.09355453758538	26.106473388813413	28.98360852586896	22.816363547732248
30	21.99867638784425	26.17400349713424	30.456106393520415	21.371213721501096
31	22.43613488726025	25.751389503584853	30.448477584383028	21.36399802477187
32	22.11938783350909	25.68727445783816	29.39672093403063	22.79661677462212
33	21.740839163496947	25.761717313788896	30.037293034126115	22.460150488588045
34	21.21158708698743	26.15778194990709	30.534212179490183	22.096418783615302
35	20.998310921067162	27.881452161720837	29.435553500397827	21.68468341681417
36	22.8093406367935	25.692424592526574	29.444752136686226	22.0534826339937
37	21.785675630196096	26.213111471773544	29.44271411547263	22.558498782557734
38	21.703934455034503	24.95259535116345	30.737160535206403	22.606309658595645
39	21.171707969186222	24.91015493643164	31.36368681342439	22.55445028095775
40	20.34327988668602	25.616632236042513	30.981833244146955	23.05825463312451
41	21.44034119780015	24.469997435948986	31.098303402421333	22.991357963829532
42	20.211497028482448	26.407136599473475	30.50110810518283	22.880258266861254
43	20.59315781196941	26.085597441787645	29.85312752256764	23.468117223675307
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	199.0
1	412.0
2	625.0
3	1789.5
4	2954.0
5	2954.0
6	4541.5
7	6129.0
8	6679.5
9	7230.0
10	10773.0
11	14316.0
12	14316.0
13	26490.0
14	38664.0
15	59672.0
16	80680.0
17	74428.0
18	68176.0
19	68176.0
20	74288.0
21	80400.0
22	63375.5
23	46351.0
24	51851.5
25	57352.0
26	57352.0
27	63374.5
28	69397.0
29	76167.0
30	82937.0
31	91944.0
32	100951.0
33	100951.0
34	112940.5
35	124930.0
36	138468.5
37	152007.0
38	163681.0
39	175355.0
40	175355.0
41	183566.5
42	191778.0
43	206691.5
44	221605.0
45	234250.0
46	246895.0
47	246895.0
48	309047.5
49	371200.0
50	354389.5
51	337579.0
52	314489.5
53	291400.0
54	291400.0
55	260533.5
56	229667.0
57	198375.0
58	167083.0
59	149406.5
60	131730.0
61	131730.0
62	116764.0
63	101798.0
64	87867.0
65	73936.0
66	63822.0
67	53708.0
68	53708.0
69	46261.0
70	38814.0
71	32617.5
72	26421.0
73	21675.5
74	16930.0
75	16930.0
76	13416.5
77	9903.0
78	7739.0
79	5575.0
80	4325.0
81	3075.0
82	3075.0
83	2266.0
84	1457.0
85	1149.5
86	842.0
87	648.0
88	454.0
89	454.0
90	376.0
91	298.0
92	197.0
93	96.0
94	71.5
95	47.0
96	47.0
97	33.0
98	19.0
99	14.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3630973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.92770913314128
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.86996999340847	32.99851712950337
2	12.391228710717007	10.638541237917778
3	4.199850995412908	5.4086994580085745
4	1.9097458555698117	3.279240584244916
5	1.0627434680486558	2.281057123976926
6	0.6481877360103568	1.6695128757073179
7	0.465335161735348	1.3983040730678788
8	0.3319499722100467	1.1399881483029772
9	0.2569225425531029	0.9926186558820044
>10	1.5592587615999556	13.032830491172708
>50	0.17075759964335507	5.094622672087298
>100	0.118021442151351	9.97637969888812
>500	0.009784014710944823	2.949376955891082
>1k	0.0056000610505912984	4.505524363160513
>5k	3.8621110693733086E-4	1.3049455311067943
>10k+	2.574740712915539E-4	3.3298410010817445
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39392	1.0848882654869645	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37605	1.0356728072613044	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28447	0.783453911665	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15063	0.41484748027594803	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8645	0.2380904512371753	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8303	0.22867148833108925	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8054	0.22181382235560548	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8007	0.22051940347669893	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7728	0.21283551268489193	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6489	0.17871242777073804	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4620	0.12723862171379408	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4470	0.12310749763217738	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4196	0.11556131097642422	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4095	0.11277968742813564	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4033	0.11107215614106743	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3835	0.1056190723533334	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3759	0.10352596948531427	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.7540827210777938E-5	0.0	0.0	0.0	0.0
6	2.7540827210777938E-5	0.0	0.0	0.0	0.0
7	2.7540827210777938E-5	0.0	0.0	0.0	0.0
8	5.5081654421555876E-5	0.0	0.0	0.0	0.0
9	5.5081654421555876E-5	0.0	0.0	1.1016330884311175E-4	0.0
10	8.262248163233381E-5	0.0	0.0	2.203266176862235E-4	0.0
11	1.1016330884311175E-4	0.0	0.0	4.1311240816166905E-4	0.0
12	1.1016330884311175E-4	0.0	0.0	7.160615074802264E-4	0.0
13	1.1016330884311175E-4	0.0	0.0	8.81306470744894E-4	0.0
14	1.6524496326466762E-4	0.0	0.0	0.0010465514340095615	0.0
15	1.6524496326466762E-4	0.0	0.0	0.001321959706117341	0.0
16	1.9278579047544555E-4	0.0	0.0	0.0020104803863867896	0.0
17	1.9278579047544555E-4	0.0	0.0	0.0028642460299209056	0.0
18	2.203266176862235E-4	0.0	0.0	0.0033324400925041305	0.0
19	2.4786744489700143E-4	0.0	0.0	0.004296369044881359	0.0
20	2.4786744489700143E-4	0.0	0.0	0.0071055334203807075	0.0
21	2.4786744489700143E-4	0.0	0.0	0.011980259836688403	0.0
22	2.754082721077794E-4	0.0	0.0	0.020490375444818784	0.0
23	2.754082721077794E-4	0.0	0.0	0.02547526516996959	0.0
24	2.754082721077794E-4	0.0	2.7540827210777938E-5	0.03431587070462931	0.0
25	2.754082721077794E-4	0.0	2.7540827210777938E-5	0.04023714855494657	0.0
26	2.754082721077794E-4	0.0	2.7540827210777938E-5	0.05020692800524818	0.0
27	3.029490993185573E-4	0.0	2.7540827210777938E-5	0.10487547001864239	0.0
28	3.3048992652933524E-4	0.0	5.5081654421555876E-5	0.23280261241270592	0.0
29	3.3048992652933524E-4	0.0	5.5081654421555876E-5	0.3894548375876108	0.0
30	3.3048992652933524E-4	0.0	8.262248163233381E-5	0.6080739239867661	0.0
31	3.3048992652933524E-4	0.0	8.262248163233381E-5	0.92223213998011	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12405	0.0	23.503426	1
ACGTTTA	150	0.0	20.966667	26
GTATTGG	1805	0.0	18.141273	1
ACCGTTA	870	0.0	16.160921	8
CTAATAC	760	0.0	16.06579	3
GTATTAG	900	0.0	15.827778	1
TTAACGG	635	0.0	15.732284	35
ATTGGAC	2145	0.0	15.610723	3
CGTTTAC	830	0.0	15.602409	28
GACCGTT	890	0.0	15.589889	7
GTATCAA	18765	0.0	15.537437	2
CCGTTTA	850	0.0	15.45294	27
TTGGACC	2615	0.0	15.210325	4
TGGACCC	2610	0.0	14.955939	5
CTCTAAT	720	0.0	14.902779	1
GCCGTTT	895	0.0	14.675978	26
TCTAATA	775	0.0	14.56129	2
CGCCGTT	920	0.0	14.47826	25
TAATACT	895	0.0	14.4692745	4
AATACTG	910	0.0	14.434066	5
>>END_MODULE