FastQCFastQC Report
Wed 25 May 2016
SRR2088609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141402
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT312700.9954154227952996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT294210.9365563528641034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT220660.7024252228781925No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124680.3968928522997057No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG59560.18959687426187416No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA57980.18456727282913807No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG57230.18217980379461146No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA55820.1776913620097014No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA54600.1738077457135381No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG46330.14748192049282455No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35430.11278403719103763No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34580.11007823895190745No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32160.10237467220050156No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA31720.10097402370024594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99800.022.1703411
TACGTTA1351.1550583E-917.81481619
TAATACT7650.015.4771244
TCTAGAC2109.094947E-1214.976193
TATAACG2151.2732926E-1114.6279072
GTATCAA151500.014.6168322
TTAACGG3750.014.30666635
GTATTGG13500.014.2518511
TGTCGGG4450.014.1348323
ATACTGG7250.013.779316
TAGCGAA4450.013.719110
CTAATAC7700.013.6948053
AGTTCCG3750.013.3232
AATACTG9350.013.2566845
CGAACTA5450.013.23853224
TTGGACC19700.013.1472074
ATTGGAC15500.013.0096773
ACGTTAA2152.904926E-912.90697720
TAACGGC4300.012.90697736
TGTGCGC2753.6379788E-1212.78181710
Produced by FastQC (version 0.11.5)