##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088609_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3141402
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.734094522127382	31.0	31.0	34.0	30.0	34.0
2	31.90904666133147	31.0	31.0	34.0	30.0	34.0
3	31.98383237802739	33.0	31.0	34.0	30.0	34.0
4	35.66688758713466	37.0	35.0	37.0	33.0	37.0
5	35.57119973820606	37.0	35.0	37.0	33.0	37.0
6	35.606680711351174	37.0	35.0	37.0	33.0	37.0
7	35.48277043180083	37.0	35.0	37.0	33.0	37.0
8	35.499688037379485	37.0	35.0	37.0	33.0	37.0
9	37.11951924650204	39.0	37.0	39.0	33.0	39.0
10	36.97871873768464	39.0	37.0	39.0	33.0	39.0
11	37.02658462686406	39.0	37.0	39.0	33.0	39.0
12	36.93194599099383	39.0	37.0	39.0	33.0	39.0
13	36.979010008906855	39.0	37.0	39.0	33.0	39.0
14	38.03421847951965	40.0	37.0	41.0	33.0	41.0
15	38.00156108641938	40.0	37.0	41.0	33.0	41.0
16	37.90140485044576	40.0	37.0	41.0	33.0	41.0
17	37.921859093487555	40.0	37.0	41.0	33.0	41.0
18	37.90462252204589	40.0	37.0	41.0	33.0	41.0
19	37.92668241759571	40.0	37.0	41.0	32.0	41.0
20	37.81034964643175	40.0	37.0	41.0	32.0	41.0
21	37.713886665889945	40.0	37.0	41.0	32.0	41.0
22	37.56772899488827	39.0	36.0	41.0	32.0	41.0
23	37.45663178415243	39.0	36.0	41.0	32.0	41.0
24	37.32318499829057	39.0	36.0	41.0	31.0	41.0
25	37.13022338433604	39.0	36.0	41.0	31.0	41.0
26	37.294612405543766	39.0	36.0	41.0	31.0	41.0
27	37.2194020376889	39.0	36.0	41.0	31.0	41.0
28	37.107859802725024	39.0	36.0	41.0	31.0	41.0
29	36.97039029070459	39.0	36.0	41.0	31.0	41.0
30	36.86306082443444	39.0	35.0	41.0	30.0	41.0
31	36.696182468846715	39.0	35.0	40.0	30.0	41.0
32	36.581569948704434	39.0	35.0	40.0	30.0	41.0
33	36.39948691698802	39.0	35.0	40.0	30.0	41.0
34	36.273941061984424	38.0	35.0	40.0	30.0	41.0
35	36.100733366821565	38.0	35.0	40.0	29.0	41.0
36	35.96718694391867	38.0	35.0	40.0	29.0	41.0
37	35.81281128617095	38.0	35.0	40.0	27.0	41.0
38	35.73582909796327	38.0	35.0	40.0	27.0	41.0
39	35.61035327538468	38.0	35.0	40.0	26.0	41.0
40	35.44245658467143	38.0	35.0	40.0	26.0	41.0
41	35.32242801144203	38.0	34.0	40.0	25.0	41.0
42	35.12138624728704	38.0	34.0	40.0	24.0	41.0
43	34.767443644589264	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	4.0
14	6.0
15	20.0
16	46.0
17	85.0
18	210.0
19	500.0
20	975.0
21	2000.0
22	3664.0
23	6405.0
24	10876.0
25	16282.0
26	23648.0
27	33602.0
28	45614.0
29	60964.0
30	77565.0
31	96580.0
32	120082.0
33	149111.0
34	185576.0
35	231112.0
36	302755.0
37	429386.0
38	603446.0
39	740882.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.38410174820032	21.420308511931935	13.81602863944188	23.37956110042586
2	18.817107775445486	22.923459016069895	35.57316128276483	22.68627192571979
3	19.591539064405	24.82159239727994	32.3560945081209	23.230774030194162
4	13.849166709641109	16.505783086660035	36.39995772588163	33.24509247781723
5	13.067254684373411	38.07720247201727	35.06173358264877	13.793809260960552
6	32.21103188958306	37.29309397523781	14.06276560593009	16.433108529249044
7	25.972607135285454	32.79281034391651	22.027362305110902	19.207220215687133
8	26.08106189529388	35.23207154003213	19.405443811393766	19.28142275328022
9	24.44863790116642	14.671315546370698	21.978435106363335	38.901611446099544
10	16.545319573871794	27.39792614889785	33.908267709767806	22.14848656746255
11	33.01602278218452	22.68245197526455	23.37956110042586	20.921964142125077
12	20.637855327016407	27.262699902782263	31.044737349756574	21.054707420444757
13	30.649085981354823	21.190602157890012	26.348235596717647	21.812076264037522
14	20.42973169304661	23.07743485233663	27.846165501900106	28.646667952716655
15	23.094147135578318	29.336232675728862	25.43252980675507	22.137090381937746
16	21.923746149012445	28.312263123280623	26.781863639228597	22.98212708847833
17	21.58061909937028	27.11101603678867	27.37074083482471	23.937624029016344
18	22.52389219845152	25.907636144625872	29.47706151584547	22.091410141077137
19	22.770915661223874	25.319745769564033	30.288196162095776	21.621142407116313
20	23.409547711499513	25.096374166693725	30.16506642575513	21.329011696051637
21	22.52924012908886	25.86488453244761	30.370866256531322	21.23500908193221
22	21.499349653434997	26.261809217667782	30.04639329827892	22.192447830618303
23	22.10936390821678	26.375993903359074	30.864053693223596	20.65058849520055
24	22.229214853750012	26.414639068797946	28.888184320249366	22.467961757202676
25	22.214189715292726	26.384747956485672	29.724180477379207	21.676881850842395
26	21.81605537909507	27.02468515650019	29.75744587926028	21.401813585144467
27	21.273686080291537	27.027868448546222	29.38818400192016	22.310261469242075
28	21.462614463223744	26.733509433049317	29.75496291146437	22.04891319226256
29	21.761843915551083	26.694195776280782	29.319902387532697	22.22405792063544
30	21.651001686508124	26.82324643582706	30.50542401131724	21.020327866347575
31	22.009313039209882	26.54709585083348	30.328942300285032	21.114648809671603
32	21.56877725295903	26.401969566454724	29.668982193300952	22.360270987285293
33	21.318284001856497	26.355334337980302	30.288800987584523	22.037580672578677
34	20.99871331335499	26.529619577500746	30.639918100262243	21.83174900888202
35	20.75633745696985	28.046490070357123	29.681524363962332	21.515648108710696
36	22.26483589174515	26.33712590747698	29.609359133278705	21.78867906749916
37	21.397102312916335	26.57714612774806	29.698172981363097	22.32757857797251
38	21.30169905029665	25.558524505937157	30.89283065331976	22.246945790446432
39	20.824778235959613	25.582431029202883	31.383630620977517	22.20916011385999
40	20.155268252837427	26.05989300318775	31.138198804228175	22.64663993974665
41	20.999413637605123	25.09086707145408	31.334448758866262	22.575270532074533
42	19.916330351862	26.747993411858783	30.759641714113634	22.576034522165582
43	20.215655302950722	26.456276528760085	30.19976430905691	23.12830385923228
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	211.0
1	438.0
2	665.0
3	1706.5
4	2748.0
5	2748.0
6	4037.0
7	5326.0
8	5832.0
9	6338.0
10	9486.5
11	12635.0
12	12635.0
13	22889.5
14	33144.0
15	49961.0
16	66778.0
17	62296.5
18	57815.0
19	57815.0
20	63947.0
21	70079.0
22	58113.5
23	46148.0
24	52307.5
25	58467.0
26	58467.0
27	64865.0
28	71263.0
29	77998.5
30	84734.0
31	93396.0
32	102058.0
33	102058.0
34	113201.5
35	124345.0
36	135812.0
37	147279.0
38	156911.5
39	166544.0
40	166544.0
41	171840.0
42	177136.0
43	187665.5
44	198195.0
45	204651.0
46	211107.0
47	211107.0
48	254108.0
49	297109.0
50	283271.5
51	269434.0
52	249542.0
53	229650.0
54	229650.0
55	207692.5
56	185735.0
57	161306.5
58	136878.0
59	122463.0
60	108048.0
61	108048.0
62	95193.5
63	82339.0
64	71926.0
65	61513.0
66	52177.5
67	42842.0
68	42842.0
69	36932.0
70	31022.0
71	26089.5
72	21157.0
73	17549.0
74	13941.0
75	13941.0
76	11063.0
77	8185.0
78	6478.0
79	4771.0
80	3699.5
81	2628.0
82	2628.0
83	2011.0
84	1394.0
85	1099.5
86	805.0
87	608.5
88	412.0
89	412.0
90	377.0
91	342.0
92	227.0
93	112.0
94	78.0
95	44.0
96	44.0
97	31.0
98	18.0
99	13.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3141402.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.90781183215011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.24142992916488	38.266171324539535
2	12.307805173844374	12.038956390182886
3	3.9916491815511295	5.856684812137758
4	1.7282408829666547	3.380979196190484
5	0.9117192685612054	2.2295097215268487
6	0.5503183366347132	1.6148919393547438
7	0.3727327780778227	1.2760681201733302
8	0.2541234501704661	0.9942897506459145
9	0.19530592134753136	0.8596786725872796
>10	1.2223743056488081	11.328264710607977
>50	0.12394625050155154	4.170495292057129
>100	0.0879711812780984	8.231228233854559
>500	0.008146934376902084	2.6913777533268113
>1k	0.0036498266007366402	3.1169528765391536
>5k	3.258773750657715E-4	0.909068754459013
>10k+	2.607019000526172E-4	3.0353824518166665
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31270	0.9954154227952996	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29421	0.9365563528641034	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22066	0.7024252228781925	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12468	0.3968928522997057	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5956	0.18959687426187416	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5798	0.18456727282913807	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5723	0.18217980379461146	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5582	0.1776913620097014	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5460	0.1738077457135381	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4633	0.14748192049282455	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3543	0.11278403719103763	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3458	0.11007823895190745	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3216	0.10237467220050156	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3172	0.10097402370024594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.183292046035496E-5	0.0	0.0	0.0
6	0.0	6.366584092070992E-5	0.0	0.0	0.0
7	0.0	6.366584092070992E-5	0.0	0.0	3.183292046035496E-5
8	0.0	6.366584092070992E-5	0.0	0.0	3.183292046035496E-5
9	3.183292046035496E-5	6.366584092070992E-5	0.0	1.9099752276212977E-4	3.183292046035496E-5
10	6.366584092070992E-5	6.366584092070992E-5	0.0	2.546633636828397E-4	3.183292046035496E-5
11	6.366584092070992E-5	6.366584092070992E-5	0.0	5.093267273656793E-4	3.183292046035496E-5
12	6.366584092070992E-5	6.366584092070992E-5	0.0	7.321571705881641E-4	6.366584092070992E-5
13	6.366584092070992E-5	6.366584092070992E-5	0.0	7.95823011508874E-4	6.366584092070992E-5
14	1.2733168184141984E-4	6.366584092070992E-5	0.0	0.0010186534547313587	6.366584092070992E-5
15	1.591646023017748E-4	9.549876138106488E-5	0.0	0.0014006485002556184	6.366584092070992E-5
16	2.2283044322248473E-4	9.549876138106488E-5	0.0	0.0019736410685420075	6.366584092070992E-5
17	2.546633636828397E-4	9.549876138106488E-5	0.0	0.002642132398209462	6.366584092070992E-5
18	2.546633636828397E-4	9.549876138106488E-5	0.0	0.0030877932846544312	6.366584092070992E-5
19	3.183292046035496E-4	9.549876138106488E-5	0.0	0.004010947978004725	6.366584092070992E-5
20	3.183292046035496E-4	9.549876138106488E-5	0.0	0.007194240024040221	6.366584092070992E-5
21	3.183292046035496E-4	9.549876138106488E-5	0.0	0.012510337740919501	6.366584092070992E-5
22	3.183292046035496E-4	9.549876138106488E-5	0.0	0.021137059185675697	6.366584092070992E-5
23	3.183292046035496E-4	9.549876138106488E-5	0.0	0.026325825220713556	6.366584092070992E-5
24	3.183292046035496E-4	9.549876138106488E-5	0.0	0.0365441926884875	6.366584092070992E-5
25	3.183292046035496E-4	9.549876138106488E-5	0.0	0.043579268110225944	6.366584092070992E-5
26	3.501621250639046E-4	9.549876138106488E-5	0.0	0.05468895735088983	6.366584092070992E-5
27	3.501621250639046E-4	9.549876138106488E-5	0.0	0.10979174266776427	6.366584092070992E-5
28	3.501621250639046E-4	1.2733168184141984E-4	0.0	0.23521344928156282	6.366584092070992E-5
29	3.8199504552425954E-4	1.2733168184141984E-4	0.0	0.3917677521055885	6.366584092070992E-5
30	4.1382796598461453E-4	1.2733168184141984E-4	0.0	0.615107522055439	6.366584092070992E-5
31	4.1382796598461453E-4	1.2733168184141984E-4	0.0	0.9509129999917234	6.366584092070992E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9980	0.0	22.170341	1
TACGTTA	135	1.1550583E-9	17.814816	19
TAATACT	765	0.0	15.477124	4
TCTAGAC	210	9.094947E-12	14.97619	3
TATAACG	215	1.2732926E-11	14.627907	2
GTATCAA	15150	0.0	14.616832	2
TTAACGG	375	0.0	14.306666	35
GTATTGG	1350	0.0	14.251851	1
TGTCGGG	445	0.0	14.13483	23
ATACTGG	725	0.0	13.77931	6
TAGCGAA	445	0.0	13.7191	10
CTAATAC	770	0.0	13.694805	3
AGTTCCG	375	0.0	13.32	32
AATACTG	935	0.0	13.256684	5
CGAACTA	545	0.0	13.238532	24
TTGGACC	1970	0.0	13.147207	4
ATTGGAC	1550	0.0	13.009677	3
ACGTTAA	215	2.904926E-9	12.906977	20
TAACGGC	430	0.0	12.906977	36
TGTGCGC	275	3.6379788E-12	12.781817	10
>>END_MODULE