FastQCFastQC Report
Wed 25 May 2016
SRR2088608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367436
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103310.7555015371834587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT97930.7161578311526097No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73360.5364784896697177No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39940.29207948306173015No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA22360.1635177075928965No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG21730.15891054499077104No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21590.15788673107918763No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA21240.15532719630022904No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG20430.14940370152606777No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG16270.11898180243901726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34800.023.709771
TCACGTT752.0676275E-417.26666624
TCTAGAC904.44695E-516.4444453
ATAGGAC803.3831532E-416.18753
ACGTTCG1302.5898044E-715.65384622
GTGCTAG1302.5898044E-715.6538461
CTAATAC4150.015.6024093
CGGTTGG2150.015.48837233
TTCGGTT2055.456968E-1215.34146331
ATTTCGG2055.456968E-1215.34146329
GCGTAAC1455.3471013E-815.31034520
TCTATGC855.365074E-415.2352943
TGTTACG1954.1836756E-1115.17948716
GTATCAA54400.015.1672792
GGCGTAA1353.9734368E-715.07407319
TTTCGGT2109.094947E-1214.976191530
ATGCTAG3600.014.90277815
ACGTTTA750.00410493514.826
ACGAATG1508.103598E-814.813
TGCGGTA1752.239176E-914.79999936
Produced by FastQC (version 0.11.5)