##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088608_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1367436
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72679525769396	31.0	31.0	34.0	30.0	34.0
2	31.91718661787462	31.0	31.0	34.0	30.0	34.0
3	31.995327752084926	31.0	31.0	34.0	30.0	34.0
4	35.69040525479803	37.0	35.0	37.0	35.0	37.0
5	35.58581900725153	37.0	35.0	37.0	33.0	37.0
6	35.61191163608388	37.0	35.0	37.0	33.0	37.0
7	35.488172024138606	37.0	35.0	37.0	33.0	37.0
8	35.501209563006974	37.0	35.0	37.0	33.0	37.0
9	37.12969528372809	39.0	37.0	39.0	33.0	39.0
10	36.97467888807959	39.0	37.0	39.0	33.0	39.0
11	37.02480993626027	39.0	37.0	39.0	33.0	39.0
12	36.92199415548516	39.0	37.0	39.0	33.0	39.0
13	36.966807221690814	39.0	37.0	39.0	33.0	39.0
14	38.04474871218836	40.0	37.0	41.0	33.0	41.0
15	37.99445092859922	40.0	37.0	41.0	33.0	41.0
16	37.88975498670504	40.0	37.0	41.0	33.0	41.0
17	37.8911144653205	40.0	37.0	41.0	33.0	41.0
18	37.86194527568384	40.0	37.0	41.0	33.0	41.0
19	37.87576603219456	40.0	37.0	41.0	32.0	41.0
20	37.75009360584335	40.0	37.0	41.0	32.0	41.0
21	37.64572016533132	39.0	37.0	41.0	32.0	41.0
22	37.4955047256325	39.0	36.0	41.0	32.0	41.0
23	37.37621212254175	39.0	36.0	41.0	31.0	41.0
24	37.24876337905394	39.0	36.0	41.0	31.0	41.0
25	37.04872184146095	39.0	36.0	41.0	31.0	41.0
26	37.226928353502466	39.0	36.0	41.0	31.0	41.0
27	37.16621545724992	39.0	36.0	41.0	31.0	41.0
28	37.06060978356574	39.0	36.0	41.0	31.0	41.0
29	36.943274858933066	39.0	36.0	41.0	30.0	41.0
30	36.84338133557987	39.0	36.0	41.0	30.0	41.0
31	36.69741984268368	39.0	35.0	40.0	30.0	41.0
32	36.59126715985246	39.0	35.0	40.0	30.0	41.0
33	36.42582029433187	39.0	35.0	40.0	30.0	41.0
34	36.29695283728087	39.0	35.0	40.0	30.0	41.0
35	36.13501107181616	38.0	35.0	40.0	29.0	41.0
36	36.01219508627826	38.0	35.0	40.0	29.0	41.0
37	35.870925586279725	38.0	35.0	40.0	28.0	41.0
38	35.81746202381684	38.0	35.0	40.0	27.0	41.0
39	35.708910691249905	38.0	35.0	40.0	27.0	41.0
40	35.56101272746951	38.0	35.0	40.0	26.0	41.0
41	35.4658901769443	38.0	34.0	40.0	26.0	41.0
42	35.29000187211687	38.0	34.0	40.0	25.0	41.0
43	34.94727577744041	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	3.0
15	4.0
16	20.0
17	34.0
18	89.0
19	210.0
20	441.0
21	886.0
22	1563.0
23	2862.0
24	4386.0
25	6870.0
26	10148.0
27	14187.0
28	19684.0
29	25821.0
30	33359.0
31	42319.0
32	52694.0
33	65527.0
34	81136.0
35	101160.0
36	132099.0
37	185611.0
38	269707.0
39	316613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43462655656279	21.049540892590223	13.24902957067095	24.26680298017604
2	19.185468277857247	22.104581128476944	35.31448638181238	23.395464211853426
3	19.68348061627747	24.372402072199357	31.59489731146467	24.3492200000585
4	14.092944752076148	16.248877461175514	36.14962601540401	33.50855177134432
5	13.451671595599354	37.475976937860345	35.16588710550256	13.906464361037738
6	32.95510722256837	36.90234862911318	14.078465098183754	16.064079050134705
7	26.887912852959843	31.951257682260813	21.893967980951214	19.26686148382813
8	26.17819042353719	34.99527583009369	19.227152129971714	19.599381616397405
9	24.928333026189158	14.40630493858579	21.194703079339728	39.47065895588533
10	16.767000430001843	26.758766040970105	33.82798171175836	22.646251817269693
11	33.88341392211409	22.19314103182891	22.98762062721765	20.935824418839346
12	21.058608958664244	26.504202024811395	30.588268847682816	21.84892016884154
13	30.47009147046004	20.91820019364709	25.848668603137554	22.763039732755317
14	20.96727013183798	22.34093588292249	26.799937986128786	29.891855999110746
15	23.845430425994344	28.727121415554368	24.627624254444083	22.79982390400721
16	22.855182984797825	27.707037111791706	26.00487335421914	23.432906549191333
17	22.432274709748757	26.503836376985834	26.637370962882358	24.426517950383055
18	22.883996033452387	25.792797615391137	28.504734408045422	22.81847194311105
19	23.443144688307168	25.034078377342706	29.007353909067774	22.515423025282352
20	23.9830602675372	24.819223715040412	28.96040472826516	22.237311289157226
21	23.197502479092257	25.521121280995967	29.107907061098288	22.173469178813487
22	22.41662498281455	25.815175262315748	28.739699700753818	23.028500054115877
23	22.838363184821812	26.027251001143746	29.570744078699114	21.563641735335327
24	23.16459417479136	26.137091607943624	27.52055672075329	23.17775749651172
25	23.098704436624455	25.877408522227004	28.414126876870288	22.609760164278256
26	22.72801067106614	26.45096370140906	28.584006856628026	22.237018770896775
27	22.296692496029063	26.439701748381644	28.0340725269775	23.229533228611796
28	22.358048201158955	26.182505067878864	28.53032975583501	22.929116975127172
29	22.830611450919825	26.240643072143776	28.042116779139935	22.88662869779646
30	22.740515826700484	26.093433257571103	29.129919060197334	22.03613185553108
31	22.878950093459586	25.897299763937763	29.08084912200644	22.142901020596213
32	22.445218642773774	25.782486346710193	28.535375695827813	23.23691931468822
33	22.283967951699385	25.877627910922342	29.05371805334948	22.784686084028795
34	22.157234415358378	26.063084488049164	29.354353695529444	22.42532740106301
35	21.957736961729836	27.289394165430775	28.440453520311003	22.31241535252838
36	23.298786926773904	25.668623613829094	28.435700098578653	22.59688936081835
37	22.29069587168979	25.91229132478595	28.647263930450855	23.1497488730734
38	22.253692311742558	25.06815675468541	29.624786827317696	23.053364106254335
39	21.799484582825084	25.17799736148529	30.139618965713932	22.88289908997569
40	21.26337174098093	25.831336896205748	29.74676694192635	23.158524420886977
41	22.05024586159791	24.61716672663291	29.9987714233061	23.33381598846308
42	20.970268444007615	26.287665382511506	29.541126604828307	23.200939568652572
43	21.463307972000152	25.79374829973761	28.943146150898468	23.79979757736377
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	72.0
1	154.5
2	237.0
3	548.5
4	860.0
5	860.0
6	1301.5
7	1743.0
8	1921.0
9	2099.0
10	3046.5
11	3994.0
12	3994.0
13	7345.5
14	10697.0
15	15980.5
16	21264.0
17	20100.0
18	18936.0
19	18936.0
20	21506.5
21	24077.0
22	20150.0
23	16223.0
24	18495.5
25	20768.0
26	20768.0
27	23383.0
28	25998.0
29	29184.0
30	32370.0
31	36792.0
32	41214.0
33	41214.0
34	46737.0
35	52260.0
36	57884.0
37	63508.0
38	68583.5
39	73659.0
40	73659.0
41	76273.5
42	78888.0
43	84656.0
44	90424.0
45	91741.5
46	93059.0
47	93059.0
48	109700.0
49	126341.0
50	122131.0
51	117921.0
52	110776.5
53	103632.0
54	103632.0
55	95130.0
56	86628.0
57	76226.0
58	65824.0
59	59173.5
60	52523.0
61	52523.0
62	47133.0
63	41743.0
64	36448.0
65	31153.0
66	27032.0
67	22911.0
68	22911.0
69	19764.0
70	16617.0
71	14267.5
72	11918.0
73	9765.5
74	7613.0
75	7613.0
76	6126.5
77	4640.0
78	3631.5
79	2623.0
80	2036.0
81	1449.0
82	1449.0
83	1101.0
84	753.0
85	579.0
86	405.0
87	296.5
88	188.0
89	188.0
90	159.0
91	130.0
92	85.5
93	41.0
94	32.0
95	23.0
96	23.0
97	16.0
98	9.0
99	5.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1367436.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.24133127927078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.23546968389168	45.98038154993561
2	9.61789595504278	10.626107533241532
3	2.7100105193013446	4.491137666011027
4	1.2240186419183223	2.7046567716085264
5	0.7086499479545251	1.9573383267996958
6	0.4381964507837137	1.4523933181886284
7	0.3250934845330237	1.257101781307488
8	0.24935905291391247	1.101994083960212
9	0.17987390808323933	0.8942826730430995
>10	1.1257774042378308	11.888720331299584
>50	0.1061753087300899	4.070058103721164
>100	0.07204917717853536	7.8059558626296015
>500	0.005042101542510806	1.8825858431763363
>1k	0.001990303240464792	1.87452306312847
>5k	2.653737653953056E-4	1.2555214494536469
>10k+	1.326868826976528E-4	0.757241642495512
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10331	0.7555015371834587	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9793	0.7161578311526097	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7336	0.5364784896697177	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3994	0.29207948306173015	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2236	0.1635177075928965	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2173	0.15891054499077104	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2159	0.15788673107918763	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2124	0.15532719630022904	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2043	0.14940370152606777	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1627	0.11898180243901726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.312956511310218E-5	0.0
10	7.312956511310218E-5	0.0	0.0	1.4625913022620437E-4	0.0
11	1.4625913022620437E-4	0.0	0.0	2.1938869533930655E-4	0.0
12	1.4625913022620437E-4	0.0	0.0	4.387773906786131E-4	0.0
13	1.4625913022620437E-4	0.0	0.0	5.119069557917153E-4	0.0
14	1.4625913022620437E-4	0.0	0.0	8.775547813572262E-4	0.0
15	1.4625913022620437E-4	0.0	0.0	0.0013894617371489415	0.0
16	1.4625913022620437E-4	0.0	0.0	0.0016088504324882481	0.0
17	1.4625913022620437E-4	0.0	0.0	0.0021938869533930654	0.0
18	1.4625913022620437E-4	0.0	0.0	0.0026326643440716785	0.0
19	2.1938869533930655E-4	0.0	0.0	0.0032908304300895983	0.0
20	2.1938869533930655E-4	0.0	0.0	0.006142883469500583	0.0
21	2.9251826045240874E-4	0.0	0.0	0.010530657376286714	0.0
22	2.9251826045240874E-4	0.0	0.0	0.01703918867135281	0.0
23	2.9251826045240874E-4	0.0	0.0	0.021573221708365144	0.0
24	2.9251826045240874E-4	0.0	0.0	0.02808175300343124	0.0
25	2.9251826045240874E-4	0.0	0.0	0.03254265647533047	0.0
26	2.9251826045240874E-4	0.0	0.0	0.03992874255175379	0.0
27	2.9251826045240874E-4	0.0	0.0	0.08563472074744266	0.0
28	2.9251826045240874E-4	0.0	0.0	0.18136132148049341	0.0
29	2.9251826045240874E-4	0.0	0.0	0.30582784130299334	0.0
30	2.9251826045240874E-4	0.0	0.0	0.4762197280165214	0.0
31	2.9251826045240874E-4	0.0	0.0	0.7399249398143679	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3480	0.0	23.70977	1
TCACGTT	75	2.0676275E-4	17.266666	24
TCTAGAC	90	4.44695E-5	16.444445	3
ATAGGAC	80	3.3831532E-4	16.1875	3
ACGTTCG	130	2.5898044E-7	15.653846	22
GTGCTAG	130	2.5898044E-7	15.653846	1
CTAATAC	415	0.0	15.602409	3
CGGTTGG	215	0.0	15.488372	33
TTCGGTT	205	5.456968E-12	15.341463	31
ATTTCGG	205	5.456968E-12	15.341463	29
GCGTAAC	145	5.3471013E-8	15.310345	20
TCTATGC	85	5.365074E-4	15.235294	3
TGTTACG	195	4.1836756E-11	15.179487	16
GTATCAA	5440	0.0	15.167279	2
GGCGTAA	135	3.9734368E-7	15.074073	19
TTTCGGT	210	9.094947E-12	14.9761915	30
ATGCTAG	360	0.0	14.902778	15
ACGTTTA	75	0.004104935	14.8	26
ACGAATG	150	8.103598E-8	14.8	13
TGCGGTA	175	2.239176E-9	14.799999	36
>>END_MODULE