FastQCFastQC Report
Wed 25 May 2016
SRR2088607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808910
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT111811.3822304088217476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT104491.2917382650727522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79540.9832985128135392No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42170.5213188117343092No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG24150.2985499004833665No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG23770.2938522208898394No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA23340.28853642556032194No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA22990.28420961540838907No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA22070.2728362858661656No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG19270.2382218046507028No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA13910.17195979775253117No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA12930.1598447293271192No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA12710.15712502008876142No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12560.1552706728807902No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12300.1520564710536401No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG11920.147358791460113No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA11330.1400650257754262No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC11270.13932328689223772No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC11100.13722169338987034No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.13351299897392788No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC9290.1148459037470176No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC9110.11262068709745213No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG8710.10767576120952888No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT8710.10767576120952888No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG8100.10013474923044591No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCTA400.001930695523.12510
ATACCGT502.7011434E-422.26
GGTATCA38500.021.719481
AGTCCGC450.003824842920.5555556
TCTATAG450.003824842920.5555553
CCCCTAT555.141283E-420.1818181
TGCACTG1600.019.656255
ATTGGAC6000.018.1916683
GTATTGG5550.018.01
TAATACT1502.5102054E-1017.2666664
ATCTATA650.001579386617.0769232
TGTTAGG1301.3911631E-817.07692319
CGATACC650.001579386617.0769234
TACCGTG650.001579386617.0769237
TTGGACC6950.017.035974
GTATTAG1757.2759576E-1216.9142861
TACTGGT1655.4569682E-1116.8181827
TGGACCC7200.016.4444455
TAGTACT1251.6558079E-716.284
GGACCCT7500.016.2799996
Produced by FastQC (version 0.11.5)