##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088607_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	808910
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.735787664882373	31.0	31.0	34.0	30.0	34.0
2	31.908764881136342	31.0	31.0	34.0	30.0	34.0
3	31.977486988663756	33.0	31.0	34.0	30.0	34.0
4	35.65477741652347	37.0	35.0	37.0	33.0	37.0
5	35.58075682090715	37.0	35.0	37.0	33.0	37.0
6	35.631345885203544	37.0	35.0	37.0	33.0	37.0
7	35.485793227924	37.0	35.0	37.0	33.0	37.0
8	35.529715295892004	37.0	35.0	37.0	33.0	37.0
9	37.135097847721006	39.0	37.0	39.0	33.0	39.0
10	37.00881062170081	39.0	37.0	39.0	33.0	39.0
11	37.05419267903722	39.0	37.0	39.0	33.0	39.0
12	36.951884634879036	39.0	37.0	39.0	33.0	39.0
13	36.98814701264665	39.0	37.0	39.0	33.0	39.0
14	37.99355676156804	40.0	37.0	41.0	33.0	41.0
15	37.959692672856065	40.0	37.0	41.0	33.0	41.0
16	37.849544448702574	40.0	37.0	41.0	32.0	41.0
17	37.91765214918841	40.0	37.0	41.0	33.0	41.0
18	37.9066533977822	40.0	37.0	41.0	33.0	41.0
19	37.9414693847276	40.0	37.0	41.0	33.0	41.0
20	37.82914415695195	40.0	37.0	41.0	32.0	41.0
21	37.73080317958735	40.0	37.0	41.0	32.0	41.0
22	37.572500030905786	39.0	36.0	41.0	32.0	41.0
23	37.42944703366258	39.0	36.0	41.0	32.0	41.0
24	37.28820264306289	39.0	36.0	41.0	31.0	41.0
25	37.08364465762569	39.0	36.0	41.0	31.0	41.0
26	37.21095795576764	39.0	36.0	41.0	31.0	41.0
27	37.13768651642334	39.0	36.0	41.0	31.0	41.0
28	36.982895501353674	39.0	35.0	41.0	31.0	41.0
29	36.833229901966845	39.0	35.0	41.0	30.0	41.0
30	36.67164455872717	39.0	35.0	40.0	30.0	41.0
31	36.454494319516385	39.0	35.0	40.0	30.0	41.0
32	36.307915590115094	38.0	35.0	40.0	30.0	41.0
33	36.066405409748924	38.0	35.0	40.0	30.0	41.0
34	35.893754558603554	38.0	35.0	40.0	29.0	41.0
35	35.680823577406635	38.0	35.0	40.0	27.0	41.0
36	35.513434127406015	38.0	35.0	40.0	27.0	41.0
37	35.310617992112846	38.0	34.0	40.0	26.0	41.0
38	35.19217959970825	38.0	34.0	40.0	25.0	41.0
39	35.015394790520574	38.0	34.0	40.0	24.0	41.0
40	34.80490536648082	38.0	34.0	40.0	23.0	41.0
41	34.63561830117071	38.0	33.0	40.0	23.0	41.0
42	34.35438676737832	38.0	33.0	40.0	21.0	41.0
43	33.96862939016702	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	3.0
15	3.0
16	13.0
17	24.0
18	48.0
19	140.0
20	302.0
21	542.0
22	1070.0
23	1796.0
24	2967.0
25	4575.0
26	6725.0
27	9468.0
28	13063.0
29	17192.0
30	21509.0
31	26334.0
32	31907.0
33	39988.0
34	49687.0
35	61860.0
36	80300.0
37	117206.0
38	150545.0
39	171640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18788245911164	20.94905490103967	14.280945964322358	22.582116675526326
2	19.081356393171056	23.036555364626473	35.419762396311086	22.462325845891385
3	20.225241374194905	24.64748859576467	32.62093434374652	22.5063356862939
4	14.039509957844507	16.070267396867386	35.45363513864336	34.43658750664474
5	12.999715666761444	38.0924948387336	34.57059499820747	14.337194496297487
6	32.40854977686022	36.13059549270005	13.443028272613763	18.017826457825965
7	25.677640281366283	32.9641122003684	21.599559901595974	19.758687616669345
8	26.95046420491773	34.443634026035035	18.821253291466295	19.784648477580944
9	24.123079205350408	14.580361226836114	22.398907171378767	38.89765239643471
10	17.172985869874275	26.950216958623336	32.88833121113598	22.98846596036642
11	32.68954519044146	22.121373205919078	23.664437329245526	21.524644274393935
12	21.12361078488336	27.27126627189675	30.78921017171255	20.815912771507335
13	32.01196672064878	20.637648193247703	26.635348802709817	20.715036283393705
14	20.339221915911534	23.39444437576492	27.87108578210184	28.395247926221707
15	22.858538032661237	29.084199725556616	25.996464377990137	22.06079786379202
16	21.56506904352771	28.02957065680978	27.204015279820993	23.201345019841515
17	21.609820622813416	26.349532086387857	27.181515867030942	24.859131423767785
18	23.1828015477618	24.923168214016393	29.83459222904897	22.059438009172837
19	22.891792659257522	24.399871431926915	30.994053726619775	21.71428218219579
20	23.989195336934888	23.714133834419158	31.02001458753137	21.276656241114587
21	22.626002892781642	24.86543620427489	31.55678629266544	20.95177461027803
22	21.05215660580287	25.36227763286398	30.927173603985608	22.658392157347542
23	22.036196857499597	25.589867846855647	32.293580249966006	20.08035504567875
24	22.082555537698877	25.626089428984685	29.51737523333869	22.77397979997775
25	22.045468593539454	25.90263440926679	30.50067374615223	21.551223251041527
26	21.7140349359014	26.712984139150215	30.62961268867983	20.943368236268558
27	20.649268769084323	26.679853135701126	30.291750627387472	22.379127467827075
28	21.018283863470597	26.289698483143987	30.488929547168414	22.20308810621701
29	21.29062565674797	26.255578494517312	29.700337491191853	22.753458357542865
30	21.319924342633914	26.490462474193667	31.544053108504038	20.64556007466838
31	21.98847832268114	25.833405446835865	31.406955038261366	20.771161192221633
32	21.664091184433374	25.853926889270745	30.158732121002334	22.323249805293543
33	21.130039188537662	25.894104412110124	30.912833318910632	22.06302308044158
34	20.300033378249744	26.485764794600144	31.535275865052974	21.678925962097143
35	20.01087883695343	28.634829585491588	30.26714962109505	21.087141956459927
36	22.219406361647152	25.728078525423097	30.461485208490437	21.591029904439306
37	21.08837818793191	26.73647253711785	30.16182269968229	22.013326575267953
38	21.08763644904872	25.008715431877466	31.732949277422705	22.17069884165111
39	20.42007145417908	25.026022672485198	32.536994226799024	22.0169116465367
40	19.258879232547503	25.83315820054147	31.957819782176017	22.950142784735014
41	20.82926407140473	24.735384653422507	31.841984893251414	22.59336638192135
42	19.333671236602342	26.86701858055902	31.308427389944494	22.49088279289414
43	19.707754880023735	26.811511787467087	30.19717891978094	23.28355441272824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34.0
1	98.0
2	162.0
3	493.0
4	824.0
5	824.0
6	1315.5
7	1807.0
8	1982.5
9	2158.0
10	3209.0
11	4260.0
12	4260.0
13	7977.0
14	11694.0
15	17773.0
16	23852.0
17	21622.0
18	19392.0
19	19392.0
20	20673.5
21	21955.0
22	16753.0
23	11551.0
24	12762.0
25	13973.0
26	13973.0
27	14703.0
28	15433.0
29	16613.0
30	17793.0
31	19174.0
32	20555.0
33	20555.0
34	22645.0
35	24735.0
36	27444.0
37	30153.0
38	32474.0
39	34795.0
40	34795.0
41	36154.5
42	37514.0
43	41041.5
44	44569.0
45	49201.0
46	53833.0
47	53833.0
48	73094.0
49	92355.0
50	86388.0
51	80421.0
52	73721.5
53	67022.0
54	67022.0
55	58453.5
56	49885.0
57	42101.0
58	34317.0
59	30078.5
60	25840.0
61	25840.0
62	22641.0
63	19442.0
64	17163.0
65	14884.0
66	12855.5
67	10827.0
68	10827.0
69	9499.5
70	8172.0
71	6896.5
72	5621.0
73	4716.0
74	3811.0
75	3811.0
76	3078.0
77	2345.0
78	1814.0
79	1283.0
80	1038.0
81	793.0
82	793.0
83	588.5
84	384.0
85	301.0
86	218.0
87	164.5
88	111.0
89	111.0
90	95.5
91	80.0
92	61.0
93	42.0
94	27.0
95	12.0
96	12.0
97	6.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	808910.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.68927741463586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.63154938699405	45.438067330687794
2	8.380213039786705	8.998551653737145
3	2.4577997793653856	3.95872482551937
4	1.1735530592627572	2.5202886303821104
5	0.701440462631268	1.8829915794030334
6	0.4684840843330927	1.5091543180860645
7	0.32293835820981887	1.2136828971227809
8	0.23576282220090758	1.0126348452161598
9	0.19531200433308862	0.943754434474298
>10	1.2429885330919614	12.690308571259354
>50	0.11471393235908185	4.267238453587939
>100	0.06485802152961148	6.212160105209281
>500	0.005770286612362662	1.9862059078451924
>1k	0.003923794896406611	3.7001573622628894
>5k	2.3081146449450648E-4	0.9856676934739618
>10k+	4.6162292898901296E-4	2.6804113917326866
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11181	1.3822304088217476	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10449	1.2917382650727522	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7954	0.9832985128135392	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4217	0.5213188117343092	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2415	0.2985499004833665	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2377	0.2938522208898394	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2334	0.28853642556032194	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2299	0.28420961540838907	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2207	0.2728362858661656	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1927	0.2382218046507028	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1391	0.17195979775253117	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1293	0.1598447293271192	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1271	0.15712502008876142	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1256	0.1552706728807902	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1230	0.1520564710536401	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1192	0.147358791460113	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1133	0.1400650257754262	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1127	0.13932328689223772	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1110	0.13722169338987034	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1080	0.13351299897392788	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	929	0.1148459037470176	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	911	0.11262068709745213	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	871	0.10767576120952888	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	871	0.10767576120952888	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	810	0.10013474923044591	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.2362314719808137E-4	0.0
5	0.0	0.0	0.0	1.2362314719808137E-4	0.0
6	0.0	0.0	0.0	1.2362314719808137E-4	0.0
7	1.2362314719808137E-4	0.0	0.0	1.2362314719808137E-4	0.0
8	1.2362314719808137E-4	0.0	0.0	1.2362314719808137E-4	0.0
9	1.2362314719808137E-4	0.0	0.0	3.708694415942441E-4	0.0
10	1.2362314719808137E-4	0.0	0.0	7.417388831884882E-4	0.0
11	1.2362314719808137E-4	0.0	0.0	0.0011126083247827323	0.0
12	1.2362314719808137E-4	0.0	0.0	0.0024724629439616275	0.0
13	1.2362314719808137E-4	0.0	0.0	0.0025960860911597087	0.0
14	1.2362314719808137E-4	0.0	0.0	0.0034614481215462785	0.0
15	1.2362314719808137E-4	0.0	0.0	0.004697679593527092	0.0
16	2.4724629439616273E-4	0.0	0.0	0.007170142537488719	0.0
17	2.4724629439616273E-4	0.0	0.0	0.009148112892658022	0.0
18	2.4724629439616273E-4	0.0	0.0	0.010631590659034998	0.0
19	2.4724629439616273E-4	0.0	0.0	0.013474923044590869	0.0
20	2.4724629439616273E-4	0.0	0.0	0.02151042761246616	0.0
21	2.4724629439616273E-4	0.0	0.0	0.03362549603787813	0.0
22	2.4724629439616273E-4	0.0	0.0	0.05402331532556156	0.0
23	2.4724629439616273E-4	0.0	0.0	0.06984707816691597	0.0
24	2.4724629439616273E-4	0.0	0.0	0.09234649095696679	0.0
25	2.4724629439616273E-4	0.0	0.0	0.10742851491513271	0.0
26	4.944925887923255E-4	0.0	0.0	0.12560111755325068	0.0
27	4.944925887923255E-4	0.0	0.0	0.2037309465824381	0.0
28	4.944925887923255E-4	0.0	0.0	0.3784104535733271	0.0
29	4.944925887923255E-4	0.0	0.0	0.6079786379201642	0.0
30	4.944925887923255E-4	0.0	0.0	0.9269263576912141	0.0
31	4.944925887923255E-4	0.0	0.0	1.3639341830364318	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGCTA	40	0.0019306955	23.125	10
ATACCGT	50	2.7011434E-4	22.2	6
GGTATCA	3850	0.0	21.71948	1
AGTCCGC	45	0.0038248429	20.555555	6
TCTATAG	45	0.0038248429	20.555555	3
CCCCTAT	55	5.141283E-4	20.181818	1
TGCACTG	160	0.0	19.65625	5
ATTGGAC	600	0.0	18.191668	3
GTATTGG	555	0.0	18.0	1
TAATACT	150	2.5102054E-10	17.266666	4
ATCTATA	65	0.0015793866	17.076923	2
TGTTAGG	130	1.3911631E-8	17.076923	19
CGATACC	65	0.0015793866	17.076923	4
TACCGTG	65	0.0015793866	17.076923	7
TTGGACC	695	0.0	17.03597	4
GTATTAG	175	7.2759576E-12	16.914286	1
TACTGGT	165	5.4569682E-11	16.818182	7
TGGACCC	720	0.0	16.444445	5
TAGTACT	125	1.6558079E-7	16.28	4
GGACCCT	750	0.0	16.279999	6
>>END_MODULE