FastQCFastQC Report
Wed 25 May 2016
SRR2088606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344915
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT173761.2919775599201437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT156131.1608912087380987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT121190.901097838896882No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63050.46880286114735875No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG34600.25726532903566396No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA33140.246409624400055No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG32430.24113048036493012No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA32080.23852808541803758No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA31030.23072090057735992No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG27720.2061096797938903No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA18780.1396370774361205No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18710.13911659844674198No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA17950.13346568370491815No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA17890.13301955885687944No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.126178977853619No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC16780.12476624916816303No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16200.12045370897045538No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG15050.11190298271637986No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC14790.10996977504154537No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA14150.105211109995799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55200.021.9519941
TCTATAC1201.0913936E-1121.5833323
CGAACTA1153.0522642E-919.30434624
ACCGTGT701.2193929E-418.58
CGAACGG500.0070354918.49999818
GTTATCG752.0675958E-417.26666611
CGGATGC650.001580122617.07692334
TCTAACG650.001580122617.0769232
CGGGTAA1851.8189894E-1217.00000226
TCGGGTA1901.8189894E-1216.55263125
CTAATAT803.3831006E-416.18753
ACCGTTA2450.015.8571428
CGCCGTC957.061104E-515.57894812
GTCGGGT2055.456968E-1215.34146424
TATCGTT855.3649937E-415.23529413
TCTAATA3050.015.1639352
CTATACA1101.4521547E-515.1363644
GCATAGG1101.4521547E-515.1363641
GTATTGG7300.014.9520551
CCGTTTA2400.014.64583327
Produced by FastQC (version 0.11.5)