FastQCFastQC Report
Wed 25 May 2016
SRR2088605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645954
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT152700.9277294505192734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT134060.8144820572142356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT110610.6720114899930375No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55800.33901311944319223No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG31910.19386933049161764No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA30890.18767231648029048No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA30370.18451305443530014No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG29000.1761896140475372No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA26020.15808461232816956No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG23870.14502227887292113No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA16660.10121789551834376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47250.021.6126981
TCTATAC1052.2611857E-819.380953
GTATTGG6850.018.905111
TAATACT4700.018.54
TCTACCG500.00703614318.4999983
ATTAGAG3450.018.2318843
TCGGGTA2050.018.0487825
GTATTAG4600.017.2934781
TATGCCG1201.0419717E-716.9583345
ATAACAC1201.0419717E-716.9583343
GTCGGGT2350.016.53191624
TATTGCG1352.221168E-816.4444459
TTAACGG3200.016.187535
CGATATA700.002593132415.85714214
CGGGTAA2350.015.74468126
AATACTG5600.015.5267855
CGACGAG1201.936638E-615.41666724
TATACCG855.3659006E-415.2352935
ACCGTTA1954.1836756E-1115.1794868
TAACACT1853.0559022E-1015.04
Produced by FastQC (version 0.11.5)