##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088605_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1645954
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83760177987963	31.0	31.0	34.0	30.0	34.0
2	32.02454807363997	31.0	31.0	34.0	30.0	34.0
3	32.10462989852693	33.0	31.0	34.0	30.0	34.0
4	35.76815937747956	37.0	35.0	37.0	35.0	37.0
5	35.68038110421069	37.0	35.0	37.0	33.0	37.0
6	35.71637542725981	37.0	35.0	37.0	33.0	37.0
7	35.597335040954974	37.0	35.0	37.0	33.0	37.0
8	35.60977949566027	37.0	35.0	37.0	33.0	37.0
9	37.252558090930854	39.0	37.0	39.0	34.0	39.0
10	37.114679389581966	39.0	37.0	39.0	33.0	39.0
11	37.15644604891753	39.0	37.0	39.0	33.0	39.0
12	37.062612320878955	39.0	37.0	39.0	33.0	39.0
13	37.099053801017526	39.0	37.0	39.0	33.0	39.0
14	38.19378245078538	40.0	37.0	41.0	33.0	41.0
15	38.14435032813797	40.0	37.0	41.0	33.0	41.0
16	38.05538672405183	40.0	37.0	41.0	33.0	41.0
17	38.064194989653416	40.0	37.0	41.0	33.0	41.0
18	38.02884406247076	40.0	37.0	41.0	33.0	41.0
19	38.0472224618671	40.0	37.0	41.0	33.0	41.0
20	37.92802836531276	40.0	37.0	41.0	33.0	41.0
21	37.825923446220244	40.0	37.0	41.0	32.0	41.0
22	37.68102267742598	40.0	37.0	41.0	32.0	41.0
23	37.55509206211109	39.0	36.0	41.0	32.0	41.0
24	37.42641774922021	39.0	36.0	41.0	32.0	41.0
25	37.22937700567574	39.0	36.0	41.0	31.0	41.0
26	37.40703203127183	39.0	36.0	41.0	32.0	41.0
27	37.34388081319405	39.0	36.0	41.0	31.0	41.0
28	37.24041984162376	39.0	36.0	41.0	31.0	41.0
29	37.122045330549945	39.0	36.0	41.0	31.0	41.0
30	37.00550015371025	39.0	36.0	41.0	31.0	41.0
31	36.854721942411516	39.0	35.0	41.0	30.0	41.0
32	36.74059056328427	39.0	35.0	41.0	30.0	41.0
33	36.55447904376428	39.0	35.0	40.0	30.0	41.0
34	36.43025017710094	39.0	35.0	40.0	30.0	41.0
35	36.25985051830124	39.0	35.0	40.0	30.0	41.0
36	36.131474512653455	38.0	35.0	40.0	29.0	41.0
37	35.981572996572204	38.0	35.0	40.0	29.0	41.0
38	35.91486031808908	38.0	35.0	40.0	28.0	41.0
39	35.80021191357717	38.0	35.0	40.0	27.0	41.0
40	35.64731092120436	38.0	35.0	40.0	26.0	41.0
41	35.533673480546845	38.0	35.0	40.0	26.0	41.0
42	35.34385165077517	38.0	34.0	40.0	25.0	41.0
43	34.997202230439	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	2.0
15	3.0
16	10.0
17	60.0
18	102.0
19	229.0
20	481.0
21	979.0
22	1660.0
23	3119.0
24	4887.0
25	7633.0
26	11032.0
27	16060.0
28	22221.0
29	29069.0
30	37926.0
31	47185.0
32	59197.0
33	74032.0
34	94313.0
35	119198.0
36	157441.0
37	227319.0
38	327189.0
39	404604.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.053848406456076	20.67263119139417	13.290347117841689	23.98317328430807
2	19.043181036651085	22.41836649140863	35.07029965600496	23.468152815935316
3	19.663003947862457	24.33427665657728	31.96772206270649	24.034997332853774
4	13.791515437247941	16.04558815130921	36.49275739176186	33.67013901968099
5	13.233358890953209	37.83106939805122	35.02479413154924	13.910777579446327
6	32.76707611512837	37.13153587524317	13.991095741436274	16.110292268192186
7	26.793762158602245	32.05873311161794	21.82533655254035	19.32216817723946
8	26.77340922042779	34.5459229115759	19.138323428236756	19.542344439759557
9	24.879431624456092	14.265526254075144	21.291846552212274	39.56319556925649
10	16.606235654216338	26.75178042642747	33.71497623870412	22.92700768065207
11	33.82372775909898	22.19156792960192	23.227076819886825	20.757627491412276
12	21.053929818208772	26.54569933303118	30.762402837503355	21.637968011256696
13	30.867995095853225	20.675304413124547	26.01706973584924	22.43963075517299
14	20.92695178601589	22.16513948749479	27.13800021142754	29.769908515061783
15	23.559832170279364	28.786102163243932	24.873963670916684	22.78010199556002
16	22.659199467299814	27.77191829176271	26.004736462865914	23.564145778071563
17	22.275652904030125	26.384759233854655	26.793944423720223	24.545643438394997
18	23.048760779462853	25.607884545983666	28.760463536648047	22.582891137905435
19	23.359644315697768	24.86235945840528	29.432049741365795	22.345946484531158
20	24.07181488668578	24.560406912951393	29.238605696149467	22.12917250421336
21	23.013158326417386	25.36808440576104	29.49256176053523	22.126195507286354
22	22.278508390878482	25.657399903034957	29.149660318575126	22.914431387511435
23	22.739456874250436	25.799505940020197	30.009161859930472	21.4518753257989
24	23.074763936294694	25.986388440989238	27.767969214206474	23.170878408509594
25	22.952038756854687	25.685043445928624	28.833308828800803	22.529608968415886
26	22.58556435963581	26.46197888883893	28.917636823386317	22.03481992813894
27	21.962764451497428	26.475223487412165	28.462763843947037	23.09924821714337
28	22.178809371343306	26.13207902529475	28.780451944586545	22.908659658775395
29	22.573048821534503	26.210149250829613	28.38262794707507	22.834173980560816
30	22.344913648862605	26.200124669340696	29.576160694648816	21.87880098714788
31	22.68149656673273	25.851269233526576	29.659516608605102	21.807717591135596
32	22.3612567544415	25.73966222628336	28.759977496333434	23.13910352294171
33	22.12340077547732	25.800660285767403	29.355741411971415	22.72019752678386
34	21.78621030721393	26.103584911850515	29.697123977948355	22.413080802987203
35	21.5454988414014	27.567416829388915	28.79873921142389	22.088345117785796
36	22.972573960147123	25.805824464110177	28.803113574255416	22.418488001487283
37	22.120241513432333	26.045381584175498	28.856578008862943	22.977798893529226
38	22.036035028925475	24.991767692171226	30.06037835808291	22.911818920820387
39	21.462021417366465	25.047662328351826	30.62722287500137	22.863093379280343
40	20.842745301509034	25.63188278651773	30.339000968435325	23.186370943537913
41	21.624541147565484	24.514962143534998	30.511606035162586	23.348890673736932
42	20.499236309155663	26.237185243330007	30.061836479026756	23.20174196848758
43	20.94226205592623	25.749869072890252	29.5640704418228	23.743798429360723
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67.0
1	158.0
2	249.0
3	708.5
4	1168.0
5	1168.0
6	1823.5
7	2479.0
8	2656.5
9	2834.0
10	4197.5
11	5561.0
12	5561.0
13	10065.5
14	14570.0
15	22727.0
16	30884.0
17	28642.5
18	26401.0
19	26401.0
20	28631.0
21	30861.0
22	24927.0
23	18993.0
24	22066.5
25	25140.0
26	25140.0
27	27672.5
28	30205.0
29	33885.0
30	37565.0
31	42234.5
32	46904.0
33	46904.0
34	53616.5
35	60329.0
36	67052.5
37	73776.0
38	79386.5
39	84997.0
40	84997.0
41	88672.0
42	92347.0
43	99271.5
44	106196.0
45	109631.0
46	113066.0
47	113066.0
48	136181.5
49	159297.0
50	152666.0
51	146035.0
52	137131.5
53	128228.0
54	128228.0
55	116840.0
56	105452.0
57	91541.5
58	77631.0
59	69612.0
60	61593.0
61	61593.0
62	54805.0
63	48017.0
64	41826.5
65	35636.0
66	30794.0
67	25952.0
68	25952.0
69	22380.5
70	18809.0
71	16153.0
72	13497.0
73	11153.5
74	8810.0
75	8810.0
76	7140.0
77	5470.0
78	4312.5
79	3155.0
80	2459.0
81	1763.0
82	1763.0
83	1352.5
84	942.0
85	738.5
86	535.0
87	403.0
88	271.0
89	271.0
90	214.0
91	157.0
92	112.0
93	67.0
94	47.0
95	27.0
96	27.0
97	18.5
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1645954.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.57811839638008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.6658217329497	42.12169422279749
2	10.253691001243794	10.577321769240987
3	3.0933944855472184	4.78654401066791
4	1.3391369850895194	2.762806638636748
5	0.761241071319802	1.963169105235998
6	0.5065302419378687	1.567552607401106
7	0.3573931238961213	1.2903565400856394
8	0.27073544079311795	1.117121969545887
9	0.20622769065656213	0.9573152620766585
>10	1.332694583183054	13.104646999960265
>50	0.12096348542097454	4.329810794686229
>100	0.08237503164814976	8.101106542833225
>500	0.0068447870628698115	2.281802908821339
>1k	0.0024782849710390693	2.2803420485281256
>5k	1.180135700494795E-4	0.3396500181722483
>10k+	3.540407101484385E-4	2.418758561310149
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15270	0.9277294505192734	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13406	0.8144820572142356	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11061	0.6720114899930375	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5580	0.33901311944319223	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3191	0.19386933049161764	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3089	0.18767231648029048	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3037	0.18451305443530014	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2900	0.1761896140475372	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2602	0.15808461232816956	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2387	0.14502227887292113	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1666	0.10121789551834376	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.075503932673696E-5	0.0
3	0.0	0.0	0.0	6.075503932673696E-5	0.0
4	0.0	0.0	0.0	1.2151007865347392E-4	0.0
5	1.2151007865347392E-4	0.0	0.0	1.2151007865347392E-4	0.0
6	1.8226511798021086E-4	0.0	0.0	1.2151007865347392E-4	0.0
7	1.8226511798021086E-4	0.0	0.0	1.8226511798021086E-4	0.0
8	1.8226511798021086E-4	0.0	0.0	1.8226511798021086E-4	0.0
9	2.4302015730694784E-4	0.0	0.0	3.6453023596042173E-4	0.0
10	4.252852752871587E-4	0.0	0.0	3.6453023596042173E-4	0.0
11	4.252852752871587E-4	0.0	0.0	6.075503932673695E-4	0.0
12	4.252852752871587E-4	0.0	0.0	7.290604719208435E-4	0.0
13	4.252852752871587E-4	0.0	0.0	7.898155112475804E-4	0.0
14	4.252852752871587E-4	0.0	0.0	9.113255899010544E-4	0.0
15	4.252852752871587E-4	0.0	0.0	0.0010328356685545283	0.0
16	4.252852752871587E-4	0.0	0.0	0.0017011411011486348	0.0
17	4.252852752871587E-4	0.0	0.0	0.0024909566123962152	0.0
18	4.252852752871587E-4	0.0	0.0	0.003098507005663585	0.0
19	5.467953539406326E-4	0.0	0.0	0.003827567477584428	0.0
20	5.467953539406326E-4	0.0	0.0	0.007169094640554961	0.0
21	5.467953539406326E-4	0.0	0.0	0.012090252826020654	0.0
22	5.467953539406326E-4	0.0	0.0	0.020231428095803406	0.0
23	5.467953539406326E-4	0.0	0.0	0.025699381635209732	0.0
24	5.467953539406326E-4	0.0	0.0	0.033658291787012275	0.0
25	5.467953539406326E-4	0.0	0.0	0.039490775562379024	0.0
26	5.467953539406326E-4	0.0	0.0	0.04890780665802325	0.0
27	5.467953539406326E-4	0.0	0.0	0.10279752654083893	0.0
28	5.467953539406326E-4	0.0	0.0	0.22060154779538188	0.0
29	5.467953539406326E-4	0.0	0.0	0.37157782052232324	0.0
30	5.467953539406326E-4	0.0	0.0	0.5743781417949712	0.0
31	5.467953539406326E-4	0.0	0.0	0.8577396452148723	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4725	0.0	21.612698	1
TCTATAC	105	2.2611857E-8	19.38095	3
GTATTGG	685	0.0	18.90511	1
TAATACT	470	0.0	18.5	4
TCTACCG	50	0.007036143	18.499998	3
ATTAGAG	345	0.0	18.231884	3
TCGGGTA	205	0.0	18.04878	25
GTATTAG	460	0.0	17.293478	1
TATGCCG	120	1.0419717E-7	16.958334	5
ATAACAC	120	1.0419717E-7	16.958334	3
GTCGGGT	235	0.0	16.531916	24
TATTGCG	135	2.221168E-8	16.444445	9
TTAACGG	320	0.0	16.1875	35
CGATATA	70	0.0025931324	15.857142	14
CGGGTAA	235	0.0	15.744681	26
AATACTG	560	0.0	15.526785	5
CGACGAG	120	1.936638E-6	15.416667	24
TATACCG	85	5.3659006E-4	15.235293	5
ACCGTTA	195	4.1836756E-11	15.179486	8
TAACACT	185	3.0559022E-10	15.0	4
>>END_MODULE