FastQCFastQC Report
Wed 25 May 2016
SRR2088604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311400
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT110610.8434497483605308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT104980.8005185298154643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83120.6338264450205887No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39380.300289766661583No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA23060.17584261095012962No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG22110.1685984444105536No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG20660.15754155863962177No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA20260.15449138325453715No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA18990.1448070764068934No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG16810.1281836205581821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33550.023.8211651
ATCCGAC500.007035399318.49999817
CCGTTTA2900.017.86206827
TAGACAG1351.1532393E-917.8148165
TCTAACC953.6071724E-617.5263163
ACCGTTA3050.016.9836068
AAGACCG1251.658118E-716.285
CGTTTAC3200.016.187528
TACCCTC1951.8189894E-1216.1282065
TAATCGC1059.345029E-615.85714223
GGACCGT3400.015.7794126
GTATCAA51300.015.5789482
GACCGTT3500.015.3285717
TACCGAA855.3648674E-415.2352947
CGTTATA1101.4521E-515.13636410
TAGTACT1853.0559022E-1015.04
TAGCGAA2600.014.94230710
GTTCTAT1001.0935507E-414.7999991
GTTATCG1001.0935507E-414.79999911
GTATTGG6150.014.7398381
Produced by FastQC (version 0.11.5)