##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088604_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1311400
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.807281531188043	31.0	31.0	34.0	30.0	34.0
2	31.996258197346346	31.0	31.0	34.0	30.0	34.0
3	32.07525773982004	33.0	31.0	34.0	30.0	34.0
4	35.749925270703066	37.0	35.0	37.0	35.0	37.0
5	35.6616676833918	37.0	35.0	37.0	33.0	37.0
6	35.68920847948757	37.0	35.0	37.0	33.0	37.0
7	35.57180646637182	37.0	35.0	37.0	33.0	37.0
8	35.5816875095318	37.0	35.0	37.0	33.0	37.0
9	37.216751563214885	39.0	37.0	39.0	33.0	39.0
10	37.077157236541105	39.0	37.0	39.0	33.0	39.0
11	37.125900564282446	39.0	37.0	39.0	33.0	39.0
12	37.02852142748208	39.0	37.0	39.0	33.0	39.0
13	37.06506252859539	39.0	37.0	39.0	33.0	39.0
14	38.14628488638097	40.0	37.0	41.0	33.0	41.0
15	38.10458517614763	40.0	37.0	41.0	33.0	41.0
16	38.0147033704438	40.0	37.0	41.0	33.0	41.0
17	38.022580448375784	40.0	37.0	41.0	33.0	41.0
18	37.99661583041025	40.0	37.0	41.0	33.0	41.0
19	38.011021808754	40.0	37.0	41.0	33.0	41.0
20	37.890915052615526	40.0	37.0	41.0	32.0	41.0
21	37.787067256367244	40.0	37.0	41.0	32.0	41.0
22	37.63678587768797	39.0	37.0	41.0	32.0	41.0
23	37.521380966905596	39.0	36.0	41.0	32.0	41.0
24	37.384886380966904	39.0	36.0	41.0	31.0	41.0
25	37.19963626658533	39.0	36.0	41.0	31.0	41.0
26	37.37654186365716	39.0	36.0	41.0	32.0	41.0
27	37.31329800213512	39.0	36.0	41.0	31.0	41.0
28	37.20568247674241	39.0	36.0	41.0	31.0	41.0
29	37.08539423516852	39.0	36.0	41.0	31.0	41.0
30	36.97904224492908	39.0	36.0	41.0	31.0	41.0
31	36.832086319963395	39.0	35.0	41.0	30.0	41.0
32	36.72268567942657	39.0	35.0	41.0	30.0	41.0
33	36.53333765441513	39.0	35.0	40.0	30.0	41.0
34	36.407063443648006	39.0	35.0	40.0	30.0	41.0
35	36.2575362208327	39.0	35.0	40.0	30.0	41.0
36	36.12444181790453	38.0	35.0	40.0	29.0	41.0
37	35.978501601342074	38.0	35.0	40.0	28.0	41.0
38	35.917098520664936	38.0	35.0	40.0	28.0	41.0
39	35.81319277108434	38.0	35.0	40.0	27.0	41.0
40	35.671041634894	38.0	35.0	40.0	27.0	41.0
41	35.561723349092574	38.0	35.0	40.0	26.0	41.0
42	35.372415738904984	38.0	34.0	40.0	25.0	41.0
43	35.03225179197804	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	2.0
14	3.0
15	6.0
16	15.0
17	30.0
18	64.0
19	173.0
20	381.0
21	737.0
22	1424.0
23	2418.0
24	3844.0
25	6097.0
26	8909.0
27	12870.0
28	17692.0
29	23490.0
30	30406.0
31	38165.0
32	48038.0
33	60253.0
34	75656.0
35	95679.0
36	125869.0
37	180890.0
38	259915.0
39	318371.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.861522037517155	20.98596919322861	13.486121701997863	23.666387067256366
2	18.862894616440446	22.667759646179654	35.43762391337502	23.03172182400488
3	19.582430989781912	24.69566875095318	32.07061156016471	23.6512886991002
4	13.790147933506178	16.291444258044837	36.8863809669056	33.03202684154339
5	13.23661735549794	37.810050327893855	35.17538508464237	13.777947231965836
6	32.19421991764527	37.403690712215955	14.320192161049262	16.081897209089526
7	26.42039042244929	32.397895378984295	22.032713131005032	19.149001067561386
8	26.359920695439985	34.93617508006711	19.506252859539426	19.197651364953487
9	24.89217630013726	14.46881195668751	21.49710233338417	39.14190940979106
10	16.515555894463933	26.90773219460119	33.79899344212292	22.777718468811955
11	33.499923745615376	22.680189110873876	23.14457831325301	20.67530883025774
12	20.76071374104011	26.70916577703218	31.19330486502974	21.336815616897972
13	30.765899039194757	21.11956687509532	26.091505261552538	22.02302882415739
14	20.723272838188194	22.729296934573735	27.271923135580295	29.275507091657772
15	23.5973005947842	29.173783742565195	24.820039652280006	22.408876010370594
16	22.46965075491841	28.202379136800364	26.063367393625132	23.264602714656093
17	21.85755680951655	26.864495958517615	26.96332164099436	24.31462559097148
18	22.776727161811806	25.74309897819125	28.884474607289917	22.59569925270703
19	23.14442580448376	25.30768644197041	29.475522342534692	22.072365411011134
20	23.989781912459968	24.940902851913986	29.243861522037516	21.82545371358853
21	23.071221595241724	25.5793045600122	29.350998932438614	21.998474912307458
22	22.0396522800061	25.939835290529206	29.440750343144728	22.579762086319963
23	22.57533933201159	26.054445630623764	29.983986579228304	21.38622845813634
24	22.75697727619338	26.181485435412537	28.27321946011896	22.788317828275126
25	22.776498398657925	26.162116821717245	28.912459966448072	22.148924813176755
26	22.47773371968888	26.611102638401707	28.88340704590514	22.02775659600427
27	21.928397132835137	26.70733567180113	28.69162726856794	22.67263992679579
28	22.026841543388745	26.370748818057038	28.82186975751106	22.78053988104316
29	22.23821869757511	26.547048955314928	28.587463779167305	22.627268567942657
30	22.18613695287479	26.541939911544915	29.600960805246302	21.670962330333996
31	22.470260789995425	26.306313863047126	29.52089370138783	21.70253164556962
32	22.14770474302272	26.029662955619948	28.92702455391185	22.895607747445478
33	21.860683239286256	26.09630928778405	29.493975903614455	22.549031569315236
34	21.481927710843372	26.233414671343603	29.950053378069242	22.334604239743786
35	21.414442580448377	27.623684611865183	29.03675461339027	21.92511819429617
36	22.70886075949367	26.16631081287174	28.951044685069395	22.1737837425652
37	21.978648772304407	26.434344974836055	28.946011895684006	22.640994357175536
38	21.742260179960347	25.53050175385085	30.101113314015553	22.626124752173247
39	21.2874027756596	25.313786792740583	30.67218240048803	22.72662803111179
40	20.779853591581514	25.74874180265365	30.508845508616744	22.962559097148088
41	21.598215647399726	24.83056275735855	30.523943876772915	23.047277718468813
42	20.44921457983834	26.175003812719233	30.34322098520665	23.032560622235778
43	20.692923593106606	25.95920390422449	29.815540643587006	23.532331859081896
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	43.0
1	132.0
2	221.0
3	588.0
4	955.0
5	955.0
6	1408.0
7	1861.0
8	2030.5
9	2200.0
10	3229.0
11	4258.0
12	4258.0
13	7691.0
14	11124.0
15	17197.5
16	23271.0
17	22050.0
18	20829.0
19	20829.0
20	23128.5
21	25428.0
22	21639.0
23	17850.0
24	20634.0
25	23418.0
26	23418.0
27	26094.0
28	28770.0
29	31988.0
30	35206.0
31	38764.0
32	42322.0
33	42322.0
34	46798.5
35	51275.0
36	56123.5
37	60972.0
38	64046.5
39	67121.0
40	67121.0
41	69335.5
42	71550.0
43	76280.5
44	81011.0
45	83862.5
46	86714.0
47	86714.0
48	103841.5
49	120969.0
50	116850.5
51	112732.0
52	106413.5
53	100095.0
54	100095.0
55	92086.0
56	84077.0
57	74541.0
58	65005.0
59	57798.5
60	50592.0
61	50592.0
62	44520.5
63	38449.0
64	33114.5
65	27780.0
66	23502.5
67	19225.0
68	19225.0
69	16433.0
70	13641.0
71	11301.5
72	8962.0
73	7409.5
74	5857.0
75	5857.0
76	4613.5
77	3370.0
78	2628.5
79	1887.0
80	1467.5
81	1048.0
82	1048.0
83	818.5
84	589.0
85	463.5
86	338.0
87	262.0
88	186.0
89	186.0
90	159.0
91	132.0
92	83.0
93	34.0
94	28.5
95	23.0
96	23.0
97	13.0
98	3.0
99	5.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1311400.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.51972747762233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.9187191803003	49.94819295882593
2	9.686164925506752	11.530357933389318
3	2.604621443539638	4.6507907550555165
4	1.099870514161726	2.618559730543131
5	0.611213007435629	1.8189615816673297
6	0.37746698178313876	1.3480039132519832
7	0.2600569104992575	1.083496150911176
8	0.20191458427802073	0.961432082398816
9	0.14916120659600726	0.799023093014491
>10	0.9464727512387462	10.726320816665625
>50	0.09114532001598935	3.806256573246574
>100	0.04793796124153228	5.419573496054822
>500	0.0030762224799364534	1.1842491762803342
>1k	0.0017944631132962648	1.8259240987627081
>5k	1.281759366640189E-4	0.6341222156311369
>10k+	2.563518733280378E-4	1.6447354243012124
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11061	0.8434497483605308	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10498	0.8005185298154643	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8312	0.6338264450205887	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3938	0.300289766661583	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2306	0.17584261095012962	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2211	0.1685984444105536	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2066	0.15754155863962177	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2026	0.15449138325453715	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1899	0.1448070764068934	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1681	0.1281836205581821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.5250876925423213E-4	0.0
5	0.0	0.0	0.0	1.5250876925423213E-4	0.0
6	0.0	0.0	0.0	1.5250876925423213E-4	0.0
7	0.0	0.0	0.0	1.5250876925423213E-4	0.0
8	0.0	0.0	0.0	1.5250876925423213E-4	0.0
9	0.0	0.0	0.0	3.812719231355803E-4	0.0
10	7.625438462711606E-5	0.0	0.0	3.812719231355803E-4	0.0
11	7.625438462711606E-5	0.0	0.0	6.100350770169285E-4	0.0
12	7.625438462711606E-5	7.625438462711606E-5	0.0	9.150526155253927E-4	0.0
13	7.625438462711606E-5	7.625438462711606E-5	0.0	9.150526155253927E-4	0.0
14	7.625438462711606E-5	1.5250876925423213E-4	0.0	0.001067561384779625	0.0
15	7.625438462711606E-5	1.5250876925423213E-4	0.0	0.0014488333079152051	0.0
16	7.625438462711606E-5	1.5250876925423213E-4	0.0	0.00213512276955925	0.0
17	7.625438462711606E-5	1.5250876925423213E-4	0.0	0.00251639469269483	0.0
18	7.625438462711606E-5	1.5250876925423213E-4	0.0	0.0032026841543388745	0.0
19	7.625438462711606E-5	1.5250876925423213E-4	0.0	0.0041939911544913834	0.0
20	7.625438462711606E-5	1.5250876925423213E-4	0.0	0.006786640231813329	0.0
21	7.625438462711606E-5	1.5250876925423213E-4	0.0	0.012276955924965685	0.0
22	7.625438462711606E-5	1.5250876925423213E-4	0.0	0.020817447003202684	0.0
23	7.625438462711606E-5	2.2876315388134817E-4	0.0	0.025697727619338112	0.0
24	7.625438462711606E-5	2.2876315388134817E-4	0.0	0.036525850236388595	0.0
25	7.625438462711606E-5	3.0501753850846426E-4	0.0	0.04232118346804941	0.0
26	7.625438462711606E-5	3.0501753850846426E-4	0.0	0.05185298154643892	0.0
27	7.625438462711606E-5	3.0501753850846426E-4	0.0	0.10980631386304712	0.0
28	7.625438462711606E-5	3.0501753850846426E-4	0.0	0.23227085557419552	0.0
29	7.625438462711606E-5	3.0501753850846426E-4	0.0	0.3834070459051395	0.0
30	7.625438462711606E-5	3.0501753850846426E-4	0.0	0.5791520512429464	0.0
31	7.625438462711606E-5	3.812719231355803E-4	0.0	0.8721213969803263	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3355	0.0	23.821165	1
ATCCGAC	50	0.0070353993	18.499998	17
CCGTTTA	290	0.0	17.862068	27
TAGACAG	135	1.1532393E-9	17.814816	5
TCTAACC	95	3.6071724E-6	17.526316	3
ACCGTTA	305	0.0	16.983606	8
AAGACCG	125	1.658118E-7	16.28	5
CGTTTAC	320	0.0	16.1875	28
TACCCTC	195	1.8189894E-12	16.128206	5
TAATCGC	105	9.345029E-6	15.857142	23
GGACCGT	340	0.0	15.779412	6
GTATCAA	5130	0.0	15.578948	2
GACCGTT	350	0.0	15.328571	7
TACCGAA	85	5.3648674E-4	15.235294	7
CGTTATA	110	1.4521E-5	15.136364	10
TAGTACT	185	3.0559022E-10	15.0	4
TAGCGAA	260	0.0	14.942307	10
GTTCTAT	100	1.0935507E-4	14.799999	1
GTTATCG	100	1.0935507E-4	14.799999	11
GTATTGG	615	0.0	14.739838	1
>>END_MODULE