FastQCFastQC Report
Wed 25 May 2016
SRR2088603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389401
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT142311.0242543369408832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT128720.9264424021574765No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT101330.7293070898898159No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51920.3736862144190194No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG35620.2563694714484875No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA35600.2562255245245973No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA33920.24413398291781857No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG29260.21059434965139653No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA27630.19886267535434338No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG24510.17640695522746852No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA19590.14099601195047362No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA17680.1272490807189573No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC16430.11825239797581837No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC15930.11465372487856278No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG15580.11213465371048388No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA15470.11134294562908764No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15330.11033531716185607No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC15240.10968755600435007No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA14180.10205836903816824No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG502.7024967E-422.1999995
GGTATCA51150.021.9902251
TACACTG1606.2937033E-1016.18755
AGTTCCG1403.4744517E-815.85714232
ACCGTTA2003.6379788E-1215.7249998
GACCGTT2500.015.5400017
GTGTTAG1201.9361105E-615.4166661
GTATCAA73700.015.2116692
TAGACTG1353.9735824E-715.0740755
GACGTAT1752.239176E-914.79999928
CGGGTAA1651.6454578E-814.57575726
TAATGGT1152.2103904E-514.4782614
TCTATAC1152.2103904E-514.4782613
GTATTGG8450.014.4497031
TAGACTT908.2755473E-414.3888885
GTACTAT1956.730261E-1014.230771
ACGTTCG1304.445281E-614.23076922
CTAGCCT1702.4372639E-814.1470594
CGGTTGG1458.913048E-714.03448333
CCGTTAT2252.7284841E-1113.97777759
Produced by FastQC (version 0.11.5)