##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088603_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1389401
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8110703821287	31.0	31.0	34.0	30.0	34.0
2	31.99120196401183	31.0	31.0	34.0	30.0	34.0
3	32.079781143096916	33.0	31.0	34.0	30.0	34.0
4	35.74099198143661	37.0	35.0	37.0	35.0	37.0
5	35.65833333933112	37.0	35.0	37.0	33.0	37.0
6	35.69794969198957	37.0	35.0	37.0	33.0	37.0
7	35.57437557623753	37.0	35.0	37.0	33.0	37.0
8	35.59570851035806	37.0	35.0	37.0	33.0	37.0
9	37.228360278997926	39.0	37.0	39.0	34.0	39.0
10	37.10146458797712	39.0	37.0	39.0	33.0	39.0
11	37.14099457248123	39.0	37.0	39.0	33.0	39.0
12	37.051854720127594	39.0	37.0	39.0	33.0	39.0
13	37.096935298016916	39.0	37.0	39.0	33.0	39.0
14	38.16923408001002	40.0	37.0	41.0	33.0	41.0
15	38.130868626120176	40.0	37.0	41.0	33.0	41.0
16	38.02954798506695	40.0	37.0	41.0	33.0	41.0
17	38.04656323120539	40.0	37.0	41.0	33.0	41.0
18	38.02239526241884	40.0	37.0	41.0	33.0	41.0
19	38.04231823641987	40.0	37.0	41.0	33.0	41.0
20	37.92455021984294	40.0	37.0	41.0	33.0	41.0
21	37.83394066939638	40.0	37.0	41.0	32.0	41.0
22	37.68155917550081	40.0	37.0	41.0	32.0	41.0
23	37.56057178597108	39.0	36.0	41.0	32.0	41.0
24	37.433967587471145	39.0	36.0	41.0	32.0	41.0
25	37.24629102757231	39.0	36.0	41.0	31.0	41.0
26	37.41904389013683	39.0	36.0	41.0	32.0	41.0
27	37.357226603406794	39.0	36.0	41.0	32.0	41.0
28	37.2439259796128	39.0	36.0	41.0	31.0	41.0
29	37.12478758832044	39.0	36.0	41.0	31.0	41.0
30	36.99463293894275	39.0	36.0	41.0	31.0	41.0
31	36.83759908046705	39.0	35.0	41.0	30.0	41.0
32	36.71796119334879	39.0	35.0	41.0	30.0	41.0
33	36.52880269986851	39.0	35.0	40.0	30.0	41.0
34	36.39086627978532	39.0	35.0	40.0	30.0	41.0
35	36.20058500029869	38.0	35.0	40.0	30.0	41.0
36	36.06066283240044	38.0	35.0	40.0	29.0	41.0
37	35.90383769696437	38.0	35.0	40.0	28.0	41.0
38	35.81814177476481	38.0	35.0	40.0	27.0	41.0
39	35.700747300455376	38.0	35.0	40.0	27.0	41.0
40	35.53250573448558	38.0	35.0	40.0	26.0	41.0
41	35.40538188759041	38.0	35.0	40.0	25.0	41.0
42	35.19132273548097	38.0	34.0	40.0	24.0	41.0
43	34.84365204861663	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	2.0
15	8.0
16	18.0
17	26.0
18	97.0
19	188.0
20	380.0
21	797.0
22	1416.0
23	2493.0
24	4169.0
25	6449.0
26	9786.0
27	13776.0
28	18901.0
29	25522.0
30	32236.0
31	40522.0
32	50739.0
33	63231.0
34	80244.0
35	101403.0
36	134211.0
37	193125.0
38	272864.0
39	336795.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.13578369383641	20.898790198078167	13.462420136447289	23.50300597163814
2	18.76722414911174	22.692800710522015	35.59368389687354	22.946291243492702
3	19.766575668219613	24.65832398278107	32.23533018905269	23.339770159946625
4	13.749738196532174	15.849204081471079	36.63679528084405	33.764262441152695
5	13.161715012440615	37.86315109892681	35.10232107217427	13.872812816458316
6	32.589151727974865	36.91972295975028	13.828765057747908	16.662360254526952
7	26.351211781192042	32.45110662796414	21.80594371243435	19.391737878409472
8	26.734110598740035	34.604048795128264	19.228861934027684	19.432978672104024
9	24.44211570309795	14.411390232193586	21.94895498131929	39.197539083389174
10	16.598375846857746	26.82263795693252	33.804999420613626	22.773986775596104
11	33.174511893974454	22.12809692810067	23.648536311691153	21.04885486623372
12	20.865034644425904	26.728856536018043	30.995155466276476	21.41095335327958
13	31.275348153628794	20.681790210313654	26.2951444543368	21.747717181720756
14	20.65501608247007	22.608303866198458	27.484361966055875	29.252318085275597
15	23.202084927245625	28.969246459445475	25.294065572142237	22.534603041166662
16	22.22187834901515	28.06317254701846	26.366254234738566	23.348694869227817
17	21.904547355299155	26.39583532759801	27.14140841988742	24.55820889721542
18	23.173079622081747	25.37913820416136	29.29111178126401	22.15667039249288
19	22.923691576441936	24.880937900577297	29.999906434499472	22.195464088481295
20	23.968962164270792	24.47529546905465	29.750446415397715	21.805295951276847
21	22.87093502883617	25.348117642063016	30.140398632216332	21.640548696884483
22	21.754770580991377	25.662785617687046	29.77750843708908	22.804935364232502
23	22.442189116029137	25.822134862433522	30.766279857291018	20.96939616424632
24	22.678693911980773	25.998325897275155	28.369491601056858	22.95348858968721
25	22.57526804716565	25.906559733295136	29.1779694990863	22.340202720452915
26	22.26693373619279	26.73886084722841	29.442759865582364	21.551445550996437
27	21.49940873801012	26.73526217413115	29.01933998895927	22.745989098899454
28	21.70611652071648	26.45305422984437	29.298812941692137	22.542016307747005
29	22.220079012466524	26.19618094416227	28.821053101300485	22.76268694207072
30	21.90224420451691	26.43628441321116	30.31810110975881	21.34337027251312
31	22.5399290773506	25.985082780277256	30.145940588786104	21.32904755358604
32	22.09232611751395	25.860064876878596	29.166309798251188	22.881299207356264
33	21.779241558052714	25.987889745293113	29.814502796528863	22.418365900125306
34	21.27197259826357	26.52020546983916	30.124780390974237	22.08304154092303
35	20.968460509241034	28.21928298597741	29.14579736159683	21.666459143184724
36	22.81731479968706	26.11175607330065	29.172427542516523	21.898501584495765
37	21.79745084392483	26.436644280520888	29.207694538869628	22.55821033668466
38	21.69352116487609	25.19661350466856	30.577997280842606	22.531868049612747
39	21.053245247412374	25.211583984753144	31.094838711070455	22.640332056764027
40	20.24059288859012	25.914116946799375	30.85315182585877	22.992138338751737
41	21.526614706625374	24.66732066552421	30.804569739045817	23.0014948888046
42	20.089952432739	26.676028014950326	30.335878554859253	22.898140997451417
43	20.48069635763901	26.39489967259272	29.706110762839526	23.41829320692874
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	69.0
1	138.5
2	208.0
3	683.5
4	1159.0
5	1159.0
6	1751.0
7	2343.0
8	2532.0
9	2721.0
10	4003.0
11	5285.0
12	5285.0
13	9505.5
14	13726.0
15	21534.0
16	29342.0
17	27337.0
18	25332.0
19	25332.0
20	27608.0
21	29884.0
22	24398.5
23	18913.0
24	21681.0
25	24449.0
26	24449.0
27	26372.5
28	28296.0
29	30939.0
30	33582.0
31	37234.0
32	40886.0
33	40886.0
34	45352.5
35	49819.0
36	54753.5
37	59688.0
38	63179.0
39	66670.0
40	66670.0
41	69625.5
42	72581.0
43	77968.0
44	83355.0
45	88128.0
46	92901.0
47	92901.0
48	117868.5
49	142836.0
50	135817.5
51	128799.0
52	119945.0
53	111091.0
54	111091.0
55	99959.5
56	88828.0
57	75658.0
58	62488.0
59	55527.5
60	48567.0
61	48567.0
62	42575.5
63	36584.0
64	32060.0
65	27536.0
66	23732.5
67	19929.0
68	19929.0
69	17351.5
70	14774.0
71	12541.0
72	10308.0
73	8652.5
74	6997.0
75	6997.0
76	5566.5
77	4136.0
78	3258.5
79	2381.0
80	1898.0
81	1415.0
82	1415.0
83	1083.0
84	751.0
85	565.0
86	379.0
87	296.0
88	213.0
89	213.0
90	163.5
91	114.0
92	76.0
93	38.0
94	29.5
95	21.0
96	21.0
97	13.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1389401.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.82614389394117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.83224483013957	43.75708084455117
2	9.883112221428043	10.44173416658253
3	2.805429386108185	4.446000493045261
4	1.208272810254762	2.5531357335061897
5	0.667606916446015	1.7633549516383775
6	0.45906427210327244	1.4550357176816902
7	0.32358192769469635	1.1965509831715229
8	0.23936874266614902	1.011594211503501
9	0.18021351646328207	0.8567986637090228
>10	1.1876288166994793	12.002376497027457
>50	0.12253780843514595	4.526740010299212
>100	0.08177651967625203	8.000980571593578
>500	0.005606738914882248	1.9501082435942494
>1k	0.0030084940518880355	2.973526783583126
>5k	1.3674972963127435E-4	0.3750680496662842
>10k+	4.10249188893823E-4	2.689914078847026
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14231	1.0242543369408832	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12872	0.9264424021574765	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10133	0.7293070898898159	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5192	0.3736862144190194	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3562	0.2563694714484875	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3560	0.2562255245245973	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3392	0.24413398291781857	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2926	0.21059434965139653	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2763	0.19886267535434338	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2451	0.17640695522746852	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1959	0.14099601195047362	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1768	0.1272490807189573	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1643	0.11825239797581837	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1593	0.11465372487856278	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1558	0.11213465371048388	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1547	0.11134294562908764	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1533	0.11033531716185607	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	1524	0.10968755600435007	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1418	0.10205836903816824	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	7.19734619451116E-5	0.0
9	0.0	0.0	0.0	2.159203858353348E-4	0.0
10	0.0	0.0	0.0	3.59867309725558E-4	0.0
11	1.439469238902232E-4	0.0	0.0	5.038142336157812E-4	0.0
12	1.439469238902232E-4	0.0	0.0	6.477611575060044E-4	0.0
13	1.439469238902232E-4	0.0	0.0	7.19734619451116E-4	0.0
14	1.439469238902232E-4	0.0	0.0	7.917080813962276E-4	0.0
15	1.439469238902232E-4	0.0	0.0	0.0010796019291766739	0.0
16	1.439469238902232E-4	0.0	0.0	0.0016553896247375667	0.0
17	1.439469238902232E-4	0.0	0.0	0.002519071168078906	0.0
18	1.439469238902232E-4	0.0	0.0	0.003022885401694687	0.0
19	1.439469238902232E-4	0.0	0.0	0.004102487330871361	0.0
20	1.439469238902232E-4	0.0	0.0	0.0076291869661818295	0.0
21	1.439469238902232E-4	0.0	0.0	0.015258373932363659	0.0
22	1.439469238902232E-4	0.0	0.0	0.025766499376349953	0.0
23	1.439469238902232E-4	0.0	0.0	0.03469120865754379	0.0
24	1.439469238902232E-4	0.0	0.0	0.04728656449793832	0.0
25	1.439469238902232E-4	0.0	0.0	0.05592337993135171	0.0
26	1.439469238902232E-4	0.0	0.0	0.0701021519345387	0.0
27	1.439469238902232E-4	0.0	0.0	0.1278248684145182	0.0
28	1.439469238902232E-4	0.0	0.0	0.2601120914696333	0.0
29	1.439469238902232E-4	0.0	0.0	0.42478737240004866	0.0
30	1.439469238902232E-4	0.0	0.0	0.6495604940546322	0.0
31	1.439469238902232E-4	0.0	0.0	0.9685468773953668	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	50	2.7024967E-4	22.199999	5
GGTATCA	5115	0.0	21.990225	1
TACACTG	160	6.2937033E-10	16.1875	5
AGTTCCG	140	3.4744517E-8	15.857142	32
ACCGTTA	200	3.6379788E-12	15.724999	8
GACCGTT	250	0.0	15.540001	7
GTGTTAG	120	1.9361105E-6	15.416666	1
GTATCAA	7370	0.0	15.211669	2
TAGACTG	135	3.9735824E-7	15.074075	5
GACGTAT	175	2.239176E-9	14.799999	28
CGGGTAA	165	1.6454578E-8	14.575757	26
TAATGGT	115	2.2103904E-5	14.478261	4
TCTATAC	115	2.2103904E-5	14.478261	3
GTATTGG	845	0.0	14.449703	1
TAGACTT	90	8.2755473E-4	14.388888	5
GTACTAT	195	6.730261E-10	14.23077	1
ACGTTCG	130	4.445281E-6	14.230769	22
CTAGCCT	170	2.4372639E-8	14.147059	4
CGGTTGG	145	8.913048E-7	14.034483	33
CCGTTAT	225	2.7284841E-11	13.9777775	9
>>END_MODULE