FastQCFastQC Report
Wed 25 May 2016
SRR2088602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564175
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62541.1085212921522578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56871.0080205609961448No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44490.7885851021402934No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23700.42008242123454603No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG13550.2401737049674303No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13170.23343820623033634No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA13140.23290645633003942No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG11760.20844596091638234No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA11550.20472371161430408No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG9600.17015996809500597No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA7330.1299242256392077No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6670.11822572783267603No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC6630.11751672796561351No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA6550.11609872823148847No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA6440.11414897859706652No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG6430.11397172863030088No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC6310.1118447290291133No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.10546373022555057No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5860.1038684805246599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC200.001841141837.08
GGGCGTC652.6788257E-622.769237
GGTATCA19800.020.7424241
TGGAGCG450.0038234320.5555555
TAACACT555.1386346E-420.1818184
GTACTGG656.89417E-519.9230771
ATTGGAC3150.018.793653
ATTAGAC701.2178013E-418.53
TTAGACT609.228869E-418.54
GTACTAG902.1482665E-618.51
TGATCGT500.007030552318.49999832
GTAATAC500.007030552318.4999983
CGGACTG500.007030552318.4999985
GTATTGG3150.018.2063481
TAGGGGT852.7191381E-517.4117644
AACTGCA650.001578586117.0769237
GACTGCT650.001578586117.0769237
TAAGTCC1201.0384065E-716.95833434
TGGACCC4000.016.655
TTGGACC4250.016.5411764
Produced by FastQC (version 0.11.5)