##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088602_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	564175
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79094784419728	31.0	31.0	34.0	30.0	34.0
2	31.971322727876988	31.0	31.0	34.0	30.0	34.0
3	32.04922586077015	33.0	31.0	34.0	30.0	34.0
4	35.72117871227899	37.0	35.0	37.0	35.0	37.0
5	35.63827713032304	37.0	35.0	37.0	33.0	37.0
6	35.67144768910356	37.0	35.0	37.0	33.0	37.0
7	35.55359950370009	37.0	35.0	37.0	33.0	37.0
8	35.575059157176405	37.0	35.0	37.0	33.0	37.0
9	37.19541099836044	39.0	37.0	39.0	33.0	39.0
10	37.07340452873665	39.0	37.0	39.0	33.0	39.0
11	37.11049408428236	39.0	37.0	39.0	33.0	39.0
12	37.02700580493641	39.0	37.0	39.0	33.0	39.0
13	37.05962511632029	39.0	37.0	39.0	33.0	39.0
14	38.12166969468693	40.0	37.0	41.0	33.0	41.0
15	38.08182744715735	40.0	37.0	41.0	33.0	41.0
16	37.98345194310276	40.0	37.0	41.0	33.0	41.0
17	38.006400496299904	40.0	37.0	41.0	33.0	41.0
18	37.98687286746134	40.0	37.0	41.0	33.0	41.0
19	38.01248548765897	40.0	37.0	41.0	33.0	41.0
20	37.89810962910445	40.0	37.0	41.0	32.0	41.0
21	37.80085611733948	40.0	37.0	41.0	32.0	41.0
22	37.65326538751274	39.0	37.0	41.0	32.0	41.0
23	37.51966322506315	39.0	36.0	41.0	32.0	41.0
24	37.3786821464971	39.0	36.0	41.0	31.0	41.0
25	37.18935259449639	39.0	36.0	41.0	31.0	41.0
26	37.35255195639651	39.0	36.0	41.0	32.0	41.0
27	37.28059555988833	39.0	36.0	41.0	31.0	41.0
28	37.16464217662959	39.0	36.0	41.0	31.0	41.0
29	37.03928568263393	39.0	36.0	41.0	31.0	41.0
30	36.897163114281916	39.0	35.0	41.0	31.0	41.0
31	36.722288297070946	39.0	35.0	40.0	30.0	41.0
32	36.602181947090884	39.0	35.0	40.0	30.0	41.0
33	36.39821686533434	39.0	35.0	40.0	30.0	41.0
34	36.24978242566579	38.0	35.0	40.0	30.0	41.0
35	36.05213985022378	38.0	35.0	40.0	29.0	41.0
36	35.91990428501795	38.0	35.0	40.0	29.0	41.0
37	35.74860637213631	38.0	35.0	40.0	27.0	41.0
38	35.66004874374086	38.0	35.0	40.0	27.0	41.0
39	35.53036912305579	38.0	35.0	40.0	26.0	41.0
40	35.36017370496743	38.0	35.0	40.0	25.0	41.0
41	35.21762219169584	38.0	34.0	40.0	24.0	41.0
42	34.993829928656886	38.0	34.0	40.0	24.0	41.0
43	34.631057739176676	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	2.0
16	12.0
17	15.0
18	30.0
19	67.0
20	175.0
21	306.0
22	590.0
23	1068.0
24	1754.0
25	2804.0
26	4137.0
27	5894.0
28	8144.0
29	10659.0
30	13613.0
31	17026.0
32	20983.0
33	26380.0
34	33224.0
35	41320.0
36	55160.0
37	79997.0
38	110261.0
39	130551.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.175034342181064	20.966721318739754	13.640271192449152	23.217973146630037
2	18.90087295608632	22.897505206717774	35.437408605485885	22.76421323171002
3	19.971285505383968	24.776532104400232	32.1258474764036	23.126334913812205
4	13.845172154030221	16.036690743120484	36.25382195240838	33.86431515044091
5	13.107280542384897	38.02295387069615	34.98949793946914	13.880267647449815
6	32.66415562547082	36.65369787743165	13.963043381929365	16.719103115168167
7	26.325342313998316	32.50055390614614	21.78437541542961	19.38972836442593
8	26.715292240882704	34.534142774848235	19.29933088137546	19.451234102893604
9	24.373642929941948	14.680905747330172	22.06584836265343	38.87960296007444
10	16.730092613107637	26.99304293880445	33.72109717729428	22.555767270793638
11	32.85594008951123	22.419816546284395	23.640182567465768	21.0840607967386
12	20.90911507954092	26.920547702397307	31.223290645633007	20.947046572428768
13	31.29419063233926	20.81056409801923	26.435414543359776	21.45983072628174
14	20.48956440820667	22.910444454291664	27.779323791376786	28.82066734612487
15	23.12934816324722	29.159746532547526	25.543492710595118	22.167412593610138
16	22.047946116010102	28.0402357424558	26.6647760003545	23.247042141179598
17	21.598794700225994	26.662294500819783	27.2862143838348	24.45269641511942
18	22.746488235033453	25.544378960428944	29.56671245624141	22.142420348296184
19	22.88172995967563	24.906101830105907	30.342535560774582	21.86963264944388
20	23.632560818894845	24.453405414986484	30.428856294589444	21.485177471529223
21	22.679487747596045	25.24057251739265	30.72681348872247	21.353126246288827
22	21.40009748748172	25.86626490007533	30.30814906722205	22.425488545220897
23	22.175211592147825	25.98076837860593	31.10541941773386	20.738600611512386
24	22.4022687995746	26.03677936810387	28.84619134133912	22.714760490982407
25	22.306731067487924	26.059644613816634	29.77710816679222	21.85651615190322
26	22.025612620197634	26.774316479815656	29.86307440067355	21.33699649931316
27	21.338591749014046	26.706075242610893	29.630699694243805	22.324633314131255
28	21.52594496388532	26.351929809013157	29.826206407586298	22.29591881951522
29	21.936633136881287	26.356715558115834	29.211326272876327	22.495325032126555
30	21.59117295165507	26.493729782425667	30.79381397615988	21.121283289759383
31	22.301768068418486	26.12558160145345	30.53875127398414	21.033899056143927
32	21.79252891390083	26.04564186644215	29.578056454114414	22.583772765542605
33	21.473478973722692	26.078787610227323	30.214561084769798	22.233172331280187
34	21.043647804316038	26.41219479771347	30.68409624673195	21.860061151238533
35	20.772632605131385	28.23769220543271	29.528426463420036	21.461248726015864
36	22.44126379226304	26.1284176009217	29.702131430850358	21.728187175964905
37	21.62236894580582	26.640847254841137	29.47507422342358	22.261709575929455
38	21.456817476846723	25.388753489608725	30.791509726591926	22.36291930695263
39	20.965303319005628	25.251739265298887	31.38724686489121	22.39571055080427
40	20.00407674923561	26.035893118270042	31.069969424380734	22.890060708113616
41	21.27921301014756	24.896530331900564	31.03310143129348	22.791155226658393
42	19.94522976026942	26.797713475428726	30.54424602295387	22.712810741347987
43	20.244959454070102	26.416803296849384	29.933974387379802	23.404262861700715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34.0
1	67.0
2	100.0
3	292.0
4	484.0
5	484.0
6	773.0
7	1062.0
8	1171.0
9	1280.0
10	1828.0
11	2376.0
12	2376.0
13	4324.0
14	6272.0
15	9662.0
16	13052.0
17	12037.0
18	11022.0
19	11022.0
20	11852.5
21	12683.0
22	10216.0
23	7749.0
24	8689.0
25	9629.0
26	9629.0
27	10656.0
28	11683.0
29	12821.5
30	13960.0
31	15217.5
32	16475.0
33	16475.0
34	18421.5
35	20368.0
36	22336.5
37	24305.0
38	25962.0
39	27619.0
40	27619.0
41	28388.0
42	29157.0
43	31480.0
44	33803.0
45	35836.0
46	37869.0
47	37869.0
48	47498.5
49	57128.0
50	54405.5
51	51683.0
52	48341.0
53	44999.0
54	44999.0
55	40496.5
56	35994.0
57	30727.0
58	25460.0
59	22679.0
60	19898.0
61	19898.0
62	17536.5
63	15175.0
64	13232.5
65	11290.0
66	9480.5
67	7671.0
68	7671.0
69	6526.0
70	5381.0
71	4474.0
72	3567.0
73	2862.5
74	2158.0
75	2158.0
76	1708.0
77	1258.0
78	987.5
79	717.0
80	539.5
81	362.0
82	362.0
83	284.0
84	206.0
85	161.5
86	117.0
87	86.5
88	56.0
89	56.0
90	51.5
91	47.0
92	31.0
93	15.0
94	12.0
95	9.0
96	9.0
97	5.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	564175.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.37811804724609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.55601243264341	54.61579279269577
2	7.208951593225928	8.993616669582593
3	1.890588492227677	3.5379405644082906
4	0.8564101259989357	2.1368500774567405
5	0.5141191806679326	1.6034893471028866
6	0.34014763062304765	1.2730661433897315
7	0.22339904050071294	0.9754648203996489
8	0.20276878831056874	1.0118668330826968
9	0.14270785650415105	0.8011662767356851
>10	0.9363950515852019	11.016395462238373
>50	0.07761161328931869	3.216338685263086
>100	0.044918071055116894	4.9732035755039155
>500	0.003411499067477124	1.3977603259920528
>1k	0.001990041122694989	2.32948231949145
>5k	5.685831779128539E-4	2.1175661066570797
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6254	1.1085212921522578	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5687	1.0080205609961448	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4449	0.7885851021402934	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2370	0.42008242123454603	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1355	0.2401737049674303	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1317	0.23343820623033634	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1314	0.23290645633003942	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1176	0.20844596091638234	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1155	0.20472371161430408	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	960	0.17015996809500597	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	733	0.1299242256392077	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	667	0.11822572783267603	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	663	0.11751672796561351	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	655	0.11609872823148847	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	644	0.11414897859706652	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	643	0.11397172863030088	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	631	0.1118447290291133	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	595	0.10546373022555057	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	586	0.1038684805246599	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.7724996676563124E-4	0.0	0.0	0.0	0.0
7	1.7724996676563124E-4	0.0	0.0	0.0	0.0
8	1.7724996676563124E-4	0.0	0.0	0.0	0.0
9	1.7724996676563124E-4	0.0	0.0	5.317499002968937E-4	0.0
10	1.7724996676563124E-4	0.0	0.0	5.317499002968937E-4	0.0
11	3.544999335312625E-4	0.0	0.0	8.862498338281561E-4	0.0
12	3.544999335312625E-4	0.0	0.0	0.0010634998005937873	0.0
13	3.544999335312625E-4	0.0	0.0	0.0010634998005937873	1.7724996676563124E-4
14	5.317499002968937E-4	0.0	0.0	0.0012407497673594186	1.7724996676563124E-4
15	5.317499002968937E-4	0.0	0.0	0.002481499534718837	1.7724996676563124E-4
16	5.317499002968937E-4	0.0	0.0	0.003722249302078256	1.7724996676563124E-4
17	5.317499002968937E-4	0.0	0.0	0.004253999202375149	3.544999335312625E-4
18	5.317499002968937E-4	0.0	0.0	0.004962999069437674	3.544999335312625E-4
19	5.317499002968937E-4	0.0	0.0	0.006203748836797093	3.544999335312625E-4
20	5.317499002968937E-4	0.0	0.0	0.010989497939469137	5.317499002968937E-4
21	5.317499002968937E-4	0.0	0.0	0.019320246377453804	7.08999867062525E-4
22	5.317499002968937E-4	0.0	0.0	0.032259493951344885	8.862498338281561E-4
23	5.317499002968937E-4	0.0	0.0	0.041121992289626444	8.862498338281561E-4
24	5.317499002968937E-4	0.0	0.0	0.05583373953117384	8.862498338281561E-4
25	5.317499002968937E-4	0.0	0.0	0.06664598750387735	8.862498338281561E-4
26	5.317499002968937E-4	0.0	0.0	0.07940798511100279	8.862498338281561E-4
27	5.317499002968937E-4	0.0	0.0	0.13860947401072363	8.862498338281561E-4
28	7.08999867062525E-4	0.0	0.0	0.27881419772233795	8.862498338281561E-4
29	7.08999867062525E-4	0.0	0.0	0.4573049142553286	0.0012407497673594186
30	8.862498338281561E-4	0.0	0.0	0.6948198697212744	0.0012407497673594186
31	0.0010634998005937873	0.0	0.0	1.023264058137989	0.00141799973412505
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGATC	20	0.0018411418	37.0	8
GGGCGTC	65	2.6788257E-6	22.76923	7
GGTATCA	1980	0.0	20.742424	1
TGGAGCG	45	0.00382343	20.555555	5
TAACACT	55	5.1386346E-4	20.181818	4
GTACTGG	65	6.89417E-5	19.923077	1
ATTGGAC	315	0.0	18.79365	3
ATTAGAC	70	1.2178013E-4	18.5	3
TTAGACT	60	9.228869E-4	18.5	4
GTACTAG	90	2.1482665E-6	18.5	1
TGATCGT	50	0.0070305523	18.499998	32
GTAATAC	50	0.0070305523	18.499998	3
CGGACTG	50	0.0070305523	18.499998	5
GTATTGG	315	0.0	18.206348	1
TAGGGGT	85	2.7191381E-5	17.411764	4
AACTGCA	65	0.0015785861	17.076923	7
GACTGCT	65	0.0015785861	17.076923	7
TAAGTCC	120	1.0384065E-7	16.958334	34
TGGACCC	400	0.0	16.65	5
TTGGACC	425	0.0	16.541176	4
>>END_MODULE