FastQCFastQC Report
Wed 25 May 2016
SRR2088601_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088601_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426434
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT130600.9155698756479446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115520.8098517001137102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95270.6678892959646222No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46930.32900225317119475No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG32710.22931309825761304No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31570.2213211406907014No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA29860.20933320434033403No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG24930.17477149310798817No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA23470.16453617903106627No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG20770.14560785847785457No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA17600.12338460805056525No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG15460.10838216138987153No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC15220.10669964400736381No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA15010.10522744129766957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA250.005496782329.614
TTACTCG555.14393E-420.1818184
GGTATCA43350.019.9296421
GTATTGG8550.019.9064331
TTAACGG1403.6379788E-1219.82142835
CAATGCG1355.638867E-1119.18518419
TAGCGCA801.6171154E-518.54
TAACGCT801.6171154E-518.532
ACGCTAA609.238305E-418.534
TCTCACG1002.8761315E-718.512
ATACGAT701.2194591E-418.56
ATTGGAC9600.017.5364573
TTGGACC11200.017.5089284
AATGCGA1603.45608E-1117.3437520
ATGCGAC1502.5102054E-1017.26666621
TATACTG1401.869921E-917.1785725
TGGACCC11450.016.641925
GGACCCT10950.016.5570776
GTATTAG3800.016.5526311
TAGGGCC904.447165E-516.4444454
Produced by FastQC (version 0.11.5)