FastQCFastQC Report
Wed 25 May 2016
SRR2088600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406866
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT179011.2724026310963519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT162641.1560447121474255No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT128370.9124536380863566No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61820.4394164049738923No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG33660.239255195590767No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA33620.23897087569107506No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA32120.22830887945262732No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG30760.2186420028631014No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA29620.21053888572188112No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG23950.17023653994054871No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA19160.13618923195243898No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17890.1271620751372199No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA17380.12353699641614767No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17100.1215467571183041No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA16560.1177084384724629No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC15470.10996072120585756No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15070.10711752220893815No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG14760.10491404298632563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49850.025.7923771
CGTATAC801.61709E-518.53
TGTTACG2050.018.0487816
TATACCG953.607549E-617.5263165
CGCTTAT852.7238188E-517.4117641
ACCGTTA3200.017.343758
TCGGGTA2150.017.20930325
GTCGGGT2150.017.20930324
GTATCAA77650.016.5344492
GTATTGG6300.016.4444451
AGTTCCG2400.016.187532
GTCTAAC1059.345995E-615.8571421
CGTACAC700.00259288115.8571423
ACCGTCC700.00259288115.8571428
TAGTACT2400.015.4166664
CCGTTTA3150.015.26984127
TAACACT855.365213E-415.2352934
CGGGTAA2500.014.79999926
CTAAGGT5050.014.6534664
CGCCGTT3850.014.41558425
Produced by FastQC (version 0.11.5)