##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088600_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1406866
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.779146699117046	31.0	31.0	34.0	30.0	34.0
2	31.95541366413006	31.0	31.0	34.0	30.0	34.0
3	32.022241634953154	33.0	31.0	34.0	30.0	34.0
4	35.69934307887176	37.0	35.0	37.0	35.0	37.0
5	35.629150182035815	37.0	35.0	37.0	33.0	37.0
6	35.67436202168508	37.0	35.0	37.0	33.0	37.0
7	35.5384855416223	37.0	35.0	37.0	33.0	37.0
8	35.56404234660586	37.0	35.0	37.0	33.0	37.0
9	37.18267482475232	39.0	37.0	39.0	33.0	39.0
10	37.05300362650032	39.0	37.0	39.0	33.0	39.0
11	37.10059238051101	39.0	37.0	39.0	33.0	39.0
12	37.00082381690936	39.0	37.0	39.0	33.0	39.0
13	37.04322231115117	39.0	37.0	39.0	33.0	39.0
14	38.073039649831614	40.0	37.0	41.0	33.0	41.0
15	38.034854065703485	40.0	37.0	41.0	33.0	41.0
16	37.930554864500245	40.0	37.0	41.0	33.0	41.0
17	37.986880058228714	40.0	37.0	41.0	33.0	41.0
18	37.96558378694204	40.0	37.0	41.0	33.0	41.0
19	37.993985923321766	40.0	37.0	41.0	33.0	41.0
20	37.8832020960063	40.0	37.0	41.0	32.0	41.0
21	37.782590523902066	40.0	37.0	41.0	32.0	41.0
22	37.62496214991335	39.0	37.0	41.0	32.0	41.0
23	37.497456047697504	39.0	36.0	41.0	32.0	41.0
24	37.358344007176235	39.0	36.0	41.0	31.0	41.0
25	37.164303494433724	39.0	36.0	41.0	31.0	41.0
26	37.31187120877184	39.0	36.0	41.0	31.0	41.0
27	37.23726424549317	39.0	36.0	41.0	31.0	41.0
28	37.10603213099186	39.0	36.0	41.0	31.0	41.0
29	36.96501799034165	39.0	36.0	41.0	31.0	41.0
30	36.829991626778956	39.0	35.0	41.0	30.0	41.0
31	36.63997352981734	39.0	35.0	40.0	30.0	41.0
32	36.50547173646957	39.0	35.0	40.0	30.0	41.0
33	36.29102203052743	38.0	35.0	40.0	30.0	41.0
34	36.14183795755957	38.0	35.0	40.0	30.0	41.0
35	35.945521464020025	38.0	35.0	40.0	29.0	41.0
36	35.799061175691214	38.0	35.0	40.0	28.0	41.0
37	35.62369976955872	38.0	35.0	40.0	27.0	41.0
38	35.52392551955908	38.0	35.0	40.0	26.0	41.0
39	35.375132386453295	38.0	34.0	40.0	26.0	41.0
40	35.19025621487761	38.0	34.0	40.0	25.0	41.0
41	35.03621382562376	38.0	34.0	40.0	24.0	41.0
42	34.79758910941056	38.0	34.0	40.0	23.0	41.0
43	34.43304124202305	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	2.0
13	1.0
14	2.0
15	11.0
16	16.0
17	33.0
18	103.0
19	202.0
20	456.0
21	892.0
22	1650.0
23	2922.0
24	4686.0
25	7154.0
26	10702.0
27	15240.0
28	20502.0
29	27439.0
30	34602.0
31	42785.0
32	53431.0
33	67377.0
34	84255.0
35	105285.0
36	138989.0
37	201987.0
38	269154.0
39	316985.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.37503785008664	20.7421318021759	14.014980815514768	22.867849532222685
2	19.1080742586714	23.07206230017642	35.19951438161132	22.62034905954085
3	19.969065994913517	24.660984059604825	32.483761779728844	22.886188165752817
4	13.70471672497594	16.178584172195503	36.25490984926781	33.86178925356075
5	13.137143125215905	38.1193375914977	34.56214024647692	14.181379036809478
6	32.182240526105545	36.804073735522785	13.64067366757033	17.37301207080134
7	26.186715721326692	32.748037126492505	21.462527348020352	19.602719804160454
8	27.17252389353357	34.33063276815276	18.869103382980327	19.627739955333343
9	24.677261373862187	14.368106130932157	21.68465226965468	39.269980225550974
10	17.202491210961103	26.825227136059866	32.813715023321336	23.15856662965769
11	33.38370534222875	22.391329380338995	23.22452884638622	21.000436431046026
12	21.127100946358787	27.0523276559388	30.59189716717868	21.22867423052373
13	31.766067272931465	20.68086086379229	26.281536407873958	21.27153545540229
14	20.775539390389703	22.775232324898038	27.639092848928044	28.81013543578422
15	23.520363702015686	28.80594171726376	25.38692384349327	22.286770737227286
16	21.94779033681957	27.938908183153195	26.70012638019541	23.413175099831825
17	22.00593375630657	26.518374884317343	26.903628348399916	24.57206301097617
18	23.186572139777347	24.97380702924088	29.42725177806557	22.412369052916198
19	23.385169589712167	24.53012582577161	30.3099229066592	21.774781677857025
20	24.05367675386284	24.11004317397677	30.251353007322656	21.584927064837732
21	22.98612661049453	24.836622677639518	30.787509258166736	21.389741453699216
22	21.748339927185672	25.257487209158512	30.381216121506956	22.612956742148864
23	22.31520272719648	25.66946674381213	31.323665509010805	20.691665019980583
24	22.406753734897283	25.599950528337455	29.22090661086415	22.772389125901114
25	22.357992872100116	25.717019247035612	30.09966834083701	21.825319540027266
26	22.058746177674347	26.31565479583699	30.0285172859391	21.597081740549562
27	21.297906126098717	26.4563931461845	29.70951035848475	22.536190369232038
28	21.55365187587162	26.147266335244435	29.759195260955913	22.539886527928033
29	21.94373877824896	26.117199505852014	29.296962184031745	22.642099531867284
30	21.715572058746176	26.311745397216225	30.88069510529077	21.09198743874683
31	22.173682497124815	25.857188957583737	30.815301528361623	21.15382701692983
32	21.842947373808165	25.726472883700367	29.831625755402435	22.598953987089033
33	21.63845028595474	25.847237761094515	30.306439987887973	22.20787196506277
34	20.88926735026648	26.28743604579256	30.873942507673085	21.949354096267875
35	20.61560944681299	27.994634883492814	29.845415270537494	21.54434039915671
36	22.472289471776275	25.818094971376095	29.836672433621963	21.872943123225667
37	21.46437542736835	26.458454465457265	29.69238008452831	22.38479002264608
38	21.339772231328357	25.18263999556461	31.061238241595145	22.416349531511887
39	20.861332920121743	25.032448008552343	31.725764927150134	22.38045414417578
40	20.026498614651288	25.652265389880768	31.266161809298115	23.055074186169826
41	21.07770036378731	24.679820252959413	31.368801293086907	22.87367809016637
42	19.814893529305564	26.44167959137544	30.924764689743018	22.81866218957598
43	20.091039231881357	26.38872501005782	30.115163775370217	23.405071982690604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	75.0
1	179.0
2	283.0
3	778.0
4	1273.0
5	1273.0
6	1904.5
7	2536.0
8	2926.0
9	3316.0
10	4859.0
11	6402.0
12	6402.0
13	11578.0
14	16754.0
15	26494.0
16	36234.0
17	33433.0
18	30632.0
19	30632.0
20	32654.0
21	34676.0
22	27239.5
23	19803.0
24	21631.0
25	23459.0
26	23459.0
27	25244.5
28	27030.0
29	29384.0
30	31738.0
31	35018.0
32	38298.0
33	38298.0
34	42311.5
35	46325.0
36	51431.0
37	56537.0
38	60550.5
39	64564.0
40	64564.0
41	67821.5
42	71079.0
43	76408.0
44	81737.0
45	87603.0
46	93469.0
47	93469.0
48	117975.5
49	142482.0
50	135811.0
51	129140.0
52	120374.5
53	111609.0
54	111609.0
55	99492.5
56	87376.0
57	75922.0
58	64468.0
59	57332.5
60	50197.0
61	50197.0
62	45124.5
63	40052.0
64	34963.0
65	29874.0
66	26137.0
67	22400.0
68	22400.0
69	19218.0
70	16036.0
71	13483.0
72	10930.0
73	8974.0
74	7018.0
75	7018.0
76	5630.0
77	4242.0
78	3275.5
79	2309.0
80	1786.5
81	1264.0
82	1264.0
83	936.0
84	608.0
85	458.5
86	309.0
87	242.0
88	175.0
89	175.0
90	139.0
91	103.0
92	67.5
93	32.0
94	23.5
95	15.0
96	15.0
97	9.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1406866.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.52264853805341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.5410848437275	46.38422292265983
2	9.723232858600902	10.797192813235005
3	2.6548802706480865	4.422179525334174
4	1.1662405710306423	2.5901106134461225
5	0.6257090187711442	1.737051096816025
6	0.4006263924700104	1.3346303030508382
7	0.2873234170020324	1.1167069969269483
8	0.19720394545841494	0.8759428283204023
9	0.15967482118204146	0.7979012079180312
>10	1.0739451636313784	11.409135626979722
>50	0.10324135222513346	4.03788266923435
>100	0.0583866834209944	5.876075763729988
>500	0.005121613231290789	1.9023343136082418
>1k	0.002816887277209934	2.937709213394872
>5k	1.2804033078226974E-4	0.4394869287613944
>10k+	3.8412099234680917E-4	3.341437176584124
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17901	1.2724026310963519	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16264	1.1560447121474255	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12837	0.9124536380863566	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6182	0.4394164049738923	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3366	0.239255195590767	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3362	0.23897087569107506	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3212	0.22830887945262732	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3076	0.2186420028631014	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2962	0.21053888572188112	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2395	0.17023653994054871	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1916	0.13618923195243898	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1789	0.1271620751372199	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1738	0.12353699641614767	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1710	0.1215467571183041	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1656	0.1177084384724629	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1547	0.10996072120585756	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1507	0.10711752220893815	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1476	0.10491404298632563	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.843198996919394E-4
2	0.0	0.0	0.0	0.0	2.843198996919394E-4
3	0.0	0.0	0.0	0.0	2.843198996919394E-4
4	0.0	0.0	0.0	0.0	2.843198996919394E-4
5	0.0	0.0	0.0	0.0	2.843198996919394E-4
6	7.107997492298485E-5	0.0	0.0	0.0	2.843198996919394E-4
7	7.107997492298485E-5	0.0	0.0	0.0	2.843198996919394E-4
8	7.107997492298485E-5	0.0	7.107997492298485E-5	7.107997492298485E-5	2.843198996919394E-4
9	7.107997492298485E-5	0.0	7.107997492298485E-5	5.686397993838788E-4	2.843198996919394E-4
10	1.421599498459697E-4	0.0	7.107997492298485E-5	7.818797241528333E-4	2.843198996919394E-4
11	2.1323992476895455E-4	0.0	1.421599498459697E-4	0.0011372795987677575	2.843198996919394E-4
12	2.1323992476895455E-4	0.0	1.421599498459697E-4	0.0017059193981516364	2.843198996919394E-4
13	2.1323992476895455E-4	0.0	1.421599498459697E-4	0.001848079347997606	2.843198996919394E-4
14	2.843198996919394E-4	0.0	1.421599498459697E-4	0.0020613192727665605	2.843198996919394E-4
15	2.843198996919394E-4	0.0	1.421599498459697E-4	0.0024877991223044697	2.843198996919394E-4
16	3.553998746149242E-4	0.0	1.421599498459697E-4	0.003411838796303273	3.553998746149242E-4
17	3.553998746149242E-4	0.0	1.421599498459697E-4	0.004620198369994015	3.553998746149242E-4
18	3.553998746149242E-4	0.0	1.421599498459697E-4	0.005615318018915803	3.553998746149242E-4
19	3.553998746149242E-4	0.0	1.421599498459697E-4	0.006610437667837591	4.264798495379091E-4
20	3.553998746149242E-4	0.0	1.421599498459697E-4	0.011372795987677575	4.264798495379091E-4
21	3.553998746149242E-4	0.0	1.421599498459697E-4	0.019191593229205908	4.264798495379091E-4
22	4.264798495379091E-4	0.0	1.421599498459697E-4	0.033336508238879896	4.264798495379091E-4
23	4.264798495379091E-4	0.0	1.421599498459697E-4	0.043500944652866726	4.264798495379091E-4
24	4.975598244608939E-4	0.0	1.421599498459697E-4	0.055797780314543105	4.264798495379091E-4
25	4.975598244608939E-4	0.0	1.421599498459697E-4	0.061555258283304874	4.264798495379091E-4
26	4.975598244608939E-4	0.0	1.421599498459697E-4	0.07442073374436514	4.264798495379091E-4
27	4.975598244608939E-4	0.0	1.421599498459697E-4	0.14038295047289506	4.264798495379091E-4
28	4.975598244608939E-4	0.0	1.421599498459697E-4	0.29384461633161935	4.264798495379091E-4
29	4.975598244608939E-4	0.0	1.421599498459697E-4	0.4945033855392056	4.975598244608939E-4
30	4.975598244608939E-4	0.0	1.421599498459697E-4	0.7471926963904167	4.975598244608939E-4
31	4.975598244608939E-4	0.0	1.421599498459697E-4	1.1132545672437886	4.975598244608939E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4985	0.0	25.792377	1
CGTATAC	80	1.61709E-5	18.5	3
TGTTACG	205	0.0	18.04878	16
TATACCG	95	3.607549E-6	17.526316	5
CGCTTAT	85	2.7238188E-5	17.411764	1
ACCGTTA	320	0.0	17.34375	8
TCGGGTA	215	0.0	17.209303	25
GTCGGGT	215	0.0	17.209303	24
GTATCAA	7765	0.0	16.534449	2
GTATTGG	630	0.0	16.444445	1
AGTTCCG	240	0.0	16.1875	32
GTCTAAC	105	9.345995E-6	15.857142	1
CGTACAC	70	0.002592881	15.857142	3
ACCGTCC	70	0.002592881	15.857142	8
TAGTACT	240	0.0	15.416666	4
CCGTTTA	315	0.0	15.269841	27
TAACACT	85	5.365213E-4	15.235293	4
CGGGTAA	250	0.0	14.799999	26
CTAAGGT	505	0.0	14.653466	4
CGCCGTT	385	0.0	14.415584	25
>>END_MODULE