FastQCFastQC Report
Wed 25 May 2016
SRR2088599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268488
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT137531.084204186401448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT125290.987711353989947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT96950.7642957599914229No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49290.38857285208847064No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG28210.22239075182421908No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG28160.22199658175717862No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA27090.2135613423225131No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA26770.21103865389345425No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA26340.20764879131690644No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG22330.17603635194026274No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA15020.11840868813894967No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA14800.11667433984397173No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA14750.11628016977693129No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14110.11123479291881358No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.10721425823500104No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC13040.10279955348414807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43800.023.7374421
ACGTTTA609.2375337E-418.526
CGGTATA500.007035275518.49999826
CTAATAC2650.018.1509443
CGGGTAA1156.406481E-817.69565226
GTATTGG4850.017.164951
TCTAATA2500.017.022
TCGGGTA1201.041426E-716.95833425
TAATACT2950.016.9322034
GACCGTT2050.016.2439027
ACCGTTA2050.016.2439028
GTATCAA65350.015.9380252
TAGCGAA1751.3096724E-1015.85714310
TCGAACC2100.015.85714231
ACACGGT957.060598E-515.5789486
CTCTAAT2650.015.3584911
ACTCACG1101.4520243E-515.1363646
AGTTCCG1853.0559022E-1015.032
ATTGGAC5600.014.8660713
TGTTACG1508.101961E-814.816
Produced by FastQC (version 0.11.5)