##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088599_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1268488
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.795989398401876	31.0	31.0	34.0	30.0	34.0
2	31.983706586108816	31.0	31.0	34.0	30.0	34.0
3	32.053155410220675	33.0	31.0	34.0	30.0	34.0
4	35.731895768820834	37.0	35.0	37.0	35.0	37.0
5	35.640926835728834	37.0	35.0	37.0	33.0	37.0
6	35.68367379115924	37.0	35.0	37.0	33.0	37.0
7	35.555389566160656	37.0	35.0	37.0	33.0	37.0
8	35.57691598186187	37.0	35.0	37.0	33.0	37.0
9	37.209046518374635	39.0	37.0	39.0	33.0	39.0
10	37.06778148472827	39.0	37.0	39.0	33.0	39.0
11	37.116824124469446	39.0	37.0	39.0	33.0	39.0
12	37.013519244959355	39.0	37.0	39.0	33.0	39.0
13	37.054042292871515	39.0	37.0	39.0	33.0	39.0
14	38.12056085670499	40.0	37.0	41.0	33.0	41.0
15	38.078821399965946	40.0	37.0	41.0	33.0	41.0
16	37.98145114498521	40.0	37.0	41.0	33.0	41.0
17	38.01522678968977	40.0	37.0	41.0	33.0	41.0
18	37.98844924035545	40.0	37.0	41.0	33.0	41.0
19	38.0109665996052	40.0	37.0	41.0	33.0	41.0
20	37.89297100169651	40.0	37.0	41.0	32.0	41.0
21	37.802022565448	40.0	37.0	41.0	32.0	41.0
22	37.6572683383682	39.0	37.0	41.0	32.0	41.0
23	37.53725301303599	39.0	36.0	41.0	32.0	41.0
24	37.40304047022912	39.0	36.0	41.0	31.0	41.0
25	37.2094296516798	39.0	36.0	41.0	31.0	41.0
26	37.375380768284764	39.0	36.0	41.0	32.0	41.0
27	37.3046595631965	39.0	36.0	41.0	31.0	41.0
28	37.1877487213123	39.0	36.0	41.0	31.0	41.0
29	37.05871793820675	39.0	36.0	41.0	31.0	41.0
30	36.941176424215286	39.0	36.0	41.0	31.0	41.0
31	36.760871998789106	39.0	35.0	41.0	30.0	41.0
32	36.65000851407345	39.0	35.0	40.0	30.0	41.0
33	36.45629599964683	39.0	35.0	40.0	30.0	41.0
34	36.328282175314236	39.0	35.0	40.0	30.0	41.0
35	36.15162382300818	38.0	35.0	40.0	30.0	41.0
36	36.01686574882853	38.0	35.0	40.0	29.0	41.0
37	35.85073173731245	38.0	35.0	40.0	28.0	41.0
38	35.771949754353216	38.0	35.0	40.0	27.0	41.0
39	35.65439483857947	38.0	35.0	40.0	27.0	41.0
40	35.48112161880916	38.0	35.0	40.0	26.0	41.0
41	35.3540892779435	38.0	35.0	40.0	25.0	41.0
42	35.143550431695054	38.0	34.0	40.0	24.0	41.0
43	34.79142490902555	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	4.0
15	6.0
16	24.0
17	33.0
18	78.0
19	188.0
20	382.0
21	759.0
22	1412.0
23	2351.0
24	4012.0
25	5978.0
26	8926.0
27	12824.0
28	17687.0
29	23313.0
30	29803.0
31	37436.0
32	46976.0
33	59604.0
34	74159.0
35	94015.0
36	122630.0
37	177605.0
38	246308.0
39	301972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.78762432123914	20.880055625279862	13.83702486740119	23.495295186079808
2	19.154694407830426	22.57262189315153	35.31637666260934	22.956307036408703
3	19.786312523256033	24.273623400457865	32.240746463506156	23.69931761277994
4	13.892129842773443	16.167121801704077	35.826669231399904	34.114079124122576
5	13.15061711265696	37.89755992961699	34.85046764336754	14.101355314358512
6	32.779261609096814	36.73649258014266	13.724528730267846	16.75971708049268
7	26.46000592831781	32.4955379948411	21.5480162208866	19.49643985595449
8	26.835176998126904	34.60962973240582	18.853627310624933	19.701565958842338
9	24.628376460794268	14.44924981552841	21.641276858748366	39.281096864928955
10	16.94371566778716	26.932142834618855	33.2625141112884	22.861627386305585
11	33.46220066725109	22.369860810665926	23.121227792458424	21.04671072962456
12	21.243322759064334	26.80364339276367	30.617711795460423	21.335322052711575
13	31.450829649157107	20.699762236615562	25.921884952794194	21.92752316143314
14	20.800433271737692	22.751180933520853	27.260092330396503	29.18829346434495
15	23.329507255882596	29.106069588360317	24.999369327892733	22.565053827864357
16	22.37790188003355	27.933965477008847	26.24534090980758	23.442791733150017
17	22.137694641179102	26.58913604228026	26.7542933003702	24.51887601617043
18	23.227259540492305	25.282068099974143	28.99215443898563	22.49851792054793
19	23.302388355270214	24.782733459047307	29.828977491312493	22.08590069436999
20	24.03381033167046	24.425536544295255	29.830396503553835	21.710256620480443
21	23.022291105631272	25.19582368930569	30.1380068238722	21.643878381190834
22	21.91435788119399	25.598744331834432	29.59499813951728	22.89189964745429
23	22.539038603439685	25.82854548091901	30.582945995547455	21.049469920093845
24	22.695129949987702	25.917233746003117	28.31189573728723	23.075740566721954
25	22.662492668436755	25.849988332566014	29.385299663851765	22.102219335145463
26	22.322166232554032	26.56099229949357	29.388295356361272	21.728546111591125
27	21.51285625090659	26.488070837091087	29.055615819779142	22.94345709222318
28	21.839465568456305	26.226263078562823	29.258376902264743	22.67589445071613
29	22.234266307604013	26.290828135544047	28.598772712079263	22.876132844772673
30	22.07273541413084	26.385507785647167	30.11530262801067	21.426454172211326
31	22.561821633314626	25.975176745858057	30.02961005543608	21.43339156539124
32	22.058939461784423	25.946638833004332	29.105517750266458	22.888903954944784
33	21.88984050302407	25.87340203454822	29.82984466545998	22.406912796967728
34	21.347225988736195	26.1188123182876	30.18924893258746	22.344712760388745
35	21.03228410517088	27.80893473174362	29.288412661373226	21.870368501712274
36	22.782951040924313	25.773440505546763	29.258534570291562	22.185073883237365
37	21.759922048927542	26.302180233474814	29.304100630041436	22.63379708755621
38	21.69843151846923	25.135436835035097	30.58988338872737	22.576248257768302
39	21.05735332143465	24.951122911686983	31.33912185215784	22.652401914720517
40	20.297787602247716	25.619004673280315	30.955673210940898	23.12753451353107
41	21.253886516861016	24.653209175017818	31.096076588820704	22.99682771930046
42	20.202240777997112	26.45945409022395	30.484009308720307	22.854295823058635
43	20.44560137738788	26.171709941284426	29.89874559317865	23.48394308814904
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	68.0
1	137.5
2	207.0
3	582.0
4	957.0
5	957.0
6	1498.5
7	2040.0
8	2223.5
9	2407.0
10	3657.0
11	4907.0
12	4907.0
13	9143.0
14	13379.0
15	20797.5
16	28216.0
17	25927.5
18	23639.0
19	23639.0
20	25523.5
21	27408.0
22	21803.5
23	16199.0
24	18189.5
25	20180.0
26	20180.0
27	22412.0
28	24644.0
29	27046.0
30	29448.0
31	32563.5
32	35679.0
33	35679.0
34	39869.5
35	44060.0
36	48499.5
37	52939.0
38	57198.5
39	61458.0
40	61458.0
41	63953.5
42	66449.0
43	71643.5
44	76838.0
45	80938.5
46	85039.0
47	85039.0
48	106737.5
49	128436.0
50	122182.5
51	115929.0
52	107366.5
53	98804.0
54	98804.0
55	89545.5
56	80287.0
57	69994.5
58	59702.0
59	53387.5
60	47073.0
61	47073.0
62	42074.0
63	37075.0
64	32471.0
65	27867.0
66	23769.5
67	19672.0
68	19672.0
69	16794.0
70	13916.0
71	11825.5
72	9735.0
73	7851.5
74	5968.0
75	5968.0
76	4800.0
77	3632.0
78	2832.5
79	2033.0
80	1534.5
81	1036.0
82	1036.0
83	800.5
84	565.0
85	420.0
86	275.0
87	211.0
88	147.0
89	147.0
90	130.5
91	114.0
92	71.0
93	28.0
94	23.0
95	18.0
96	18.0
97	12.5
98	7.0
99	7.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1268488.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.96484029290831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.66159871306263	45.98446411912871
2	9.424655011371494	10.360493150315842
3	2.6017321253799386	4.290113722693117
4	1.2147134857367843	2.6706613098065723
5	0.6268928733427345	1.72285333320229
6	0.412417865790723	1.3601089276277507
7	0.3128796782039785	1.2038167080364757
8	0.22178012092193425	0.9752087141292809
9	0.17763429139162323	0.8787276411196057
>10	1.1623666102755672	12.264073703233072
>50	0.10945780964093568	4.158825481249282
>100	0.06567945536728084	6.605618796218401
>500	0.005030150579003664	1.8278581862814711
>1k	0.0027306531714591316	2.8551841602903103
>5k	1.4371858797153326E-4	0.7658535423310802
>10k+	2.874371759430665E-4	2.076138504336818
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13753	1.084204186401448	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12529	0.987711353989947	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9695	0.7642957599914229	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4929	0.38857285208847064	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2821	0.22239075182421908	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2816	0.22199658175717862	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2709	0.2135613423225131	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2677	0.21103865389345425	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2634	0.20764879131690644	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2233	0.17603635194026274	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1502	0.11840868813894967	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1480	0.11667433984397173	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1475	0.11628016977693129	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1411	0.11123479291881358	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1360	0.10721425823500104	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1304	0.10279955348414807	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.8834013408089E-5	0.0
2	0.0	0.0	0.0	1.57668026816178E-4	0.0
3	0.0	0.0	0.0	1.57668026816178E-4	0.0
4	0.0	0.0	0.0	1.57668026816178E-4	0.0
5	0.0	0.0	0.0	1.57668026816178E-4	0.0
6	0.0	0.0	0.0	1.57668026816178E-4	0.0
7	0.0	0.0	0.0	3.15336053632356E-4	0.0
8	0.0	0.0	0.0	3.15336053632356E-4	0.0
9	0.0	0.0	0.0	4.73004080448534E-4	0.0
10	7.8834013408089E-5	0.0	0.0	5.51838093856623E-4	0.0
11	7.8834013408089E-5	0.0	0.0	7.883401340808901E-4	0.0
12	7.8834013408089E-5	0.0	0.0	0.001419012241345602	0.0
13	1.57668026816178E-4	0.0	0.0	0.001497846254753691	0.0
14	1.57668026816178E-4	0.0	0.0	0.001734348294977958	0.0
15	1.57668026816178E-4	0.0	0.0	0.00236502040224267	0.0
16	1.57668026816178E-4	0.0	0.0	0.003074526522915471	0.0
17	1.57668026816178E-4	0.0	0.0	0.00394170067040445	0.0
18	1.57668026816178E-4	0.0	0.0	0.00473004080448534	0.0
19	1.57668026816178E-4	0.0	0.0	0.005676048965382408	0.0
20	1.57668026816178E-4	0.0	0.0	0.009696583649194948	0.0
21	1.57668026816178E-4	0.0	0.0	0.016791644855922955	0.0
22	1.57668026816178E-4	0.0	0.0	0.028932082920768664	0.0
23	1.57668026816178E-4	0.0	0.0	0.03500230195319152	0.0
24	1.57668026816178E-4	0.0	0.0	0.044383549548754106	0.0
25	1.57668026816178E-4	0.0	0.0	0.051636278782298296	0.0
26	1.57668026816178E-4	0.0	0.0	0.06385555086055208	0.0
27	1.57668026816178E-4	0.0	0.0	0.12983962008312258	0.0
28	1.57668026816178E-4	0.0	0.0	0.2838812822825285	0.0
29	1.57668026816178E-4	0.0	0.0	0.4723734083412693	0.0
30	3.15336053632356E-4	0.0	0.0	0.7430105763712388	0.0
31	3.15336053632356E-4	0.0	0.0	1.1368653073580515	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4380	0.0	23.737442	1
ACGTTTA	60	9.2375337E-4	18.5	26
CGGTATA	50	0.0070352755	18.499998	26
CTAATAC	265	0.0	18.150944	3
CGGGTAA	115	6.406481E-8	17.695652	26
GTATTGG	485	0.0	17.16495	1
TCTAATA	250	0.0	17.02	2
TCGGGTA	120	1.041426E-7	16.958334	25
TAATACT	295	0.0	16.932203	4
GACCGTT	205	0.0	16.243902	7
ACCGTTA	205	0.0	16.243902	8
GTATCAA	6535	0.0	15.938025	2
TAGCGAA	175	1.3096724E-10	15.857143	10
TCGAACC	210	0.0	15.857142	31
ACACGGT	95	7.060598E-5	15.578948	6
CTCTAAT	265	0.0	15.358491	1
ACTCACG	110	1.4520243E-5	15.136364	6
AGTTCCG	185	3.0559022E-10	15.0	32
ATTGGAC	560	0.0	14.866071	3
TGTTACG	150	8.101961E-8	14.8	16
>>END_MODULE