FastQCFastQC Report
Wed 25 May 2016
SRR2088598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577108
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55730.9656771349556755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55310.9583994676906229No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39700.6879128343394997No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22040.3819042536232386No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA11540.19996257199692258No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG11490.19909618303679727No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG11330.19632373836439626No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA11160.1933780158999702No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA10580.18332790396251655No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG9420.16322768008760924No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.11696250961691745No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.11592284286476708No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA6050.10483306417516304No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6040.104659786383138No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA5950.10310028625491242No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA5880.10188734171073699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTG250.005494103329.67
TAATACT901.4188117E-1024.6666664
GATATAA400.001930028123.1250021
GGTATCA19050.023.0157471
AATACTG1400.021.1428575
ATAAGAC450.003823534820.5555553
CCGACCG555.13883E-420.1818189
TAACACT656.894521E-519.9230774
TAAGACT1500.019.7333324
TTAACGG1254.110916E-1019.2435
AACGGCC1551.8189894E-1219.09677537
GTATTAG1800.018.51
GCTATAC500.007030740418.53
TAACGGC1306.9303496E-1018.536
TACGGCT500.007030740418.54
ACTTAGC500.007030740418.58
GTTTAAC1351.1477823E-917.81481633
CTAATAC1258.552888E-917.763
TTAGACT752.0650205E-417.2666664
TTTACAC650.001578645517.0769233
Produced by FastQC (version 0.11.5)