##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088598_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	577108
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.773328389140335	31.0	31.0	34.0	30.0	34.0
2	31.961171912363024	31.0	31.0	34.0	30.0	34.0
3	32.028944322379864	33.0	31.0	34.0	30.0	34.0
4	35.71563901384143	37.0	35.0	37.0	35.0	37.0
5	35.62632817427587	37.0	35.0	37.0	33.0	37.0
6	35.662205341114664	37.0	35.0	37.0	33.0	37.0
7	35.53160067093161	37.0	35.0	37.0	33.0	37.0
8	35.55625810073678	37.0	35.0	37.0	33.0	37.0
9	37.184812894640174	39.0	37.0	39.0	33.0	39.0
10	37.041113621713784	39.0	37.0	39.0	33.0	39.0
11	37.086134657637736	39.0	37.0	39.0	33.0	39.0
12	36.98858099350555	39.0	37.0	39.0	33.0	39.0
13	37.02800342396917	39.0	37.0	39.0	33.0	39.0
14	38.08926232178379	40.0	37.0	41.0	33.0	41.0
15	38.040326940537994	40.0	37.0	41.0	33.0	41.0
16	37.93257761112305	40.0	37.0	41.0	33.0	41.0
17	37.9639252964783	40.0	37.0	41.0	33.0	41.0
18	37.94807904239761	40.0	37.0	41.0	33.0	41.0
19	37.97135718097826	40.0	37.0	41.0	33.0	41.0
20	37.845306251169625	40.0	37.0	41.0	32.0	41.0
21	37.744489766213604	40.0	37.0	41.0	32.0	41.0
22	37.595124309488	39.0	37.0	41.0	32.0	41.0
23	37.479835663342044	39.0	36.0	41.0	32.0	41.0
24	37.336358185989454	39.0	36.0	41.0	31.0	41.0
25	37.132990705379235	39.0	36.0	41.0	31.0	41.0
26	37.29938763628299	39.0	36.0	41.0	31.0	41.0
27	37.21940780581797	39.0	36.0	41.0	31.0	41.0
28	37.104587009710485	39.0	36.0	41.0	31.0	41.0
29	36.96764903622892	39.0	36.0	41.0	31.0	41.0
30	36.84415568663058	39.0	35.0	41.0	30.0	41.0
31	36.6825048344504	39.0	35.0	40.0	30.0	41.0
32	36.57109934362372	39.0	35.0	40.0	30.0	41.0
33	36.376537840404225	39.0	35.0	40.0	30.0	41.0
34	36.22560248688287	38.0	35.0	40.0	30.0	41.0
35	36.04600005544889	38.0	35.0	40.0	29.0	41.0
36	35.91688730705518	38.0	35.0	40.0	29.0	41.0
37	35.758771321832306	38.0	35.0	40.0	27.0	41.0
38	35.670741005149814	38.0	35.0	40.0	27.0	41.0
39	35.53338716496739	38.0	35.0	40.0	26.0	41.0
40	35.37596602369054	38.0	34.0	40.0	25.0	41.0
41	35.24848381931978	38.0	34.0	40.0	25.0	41.0
42	35.031981882074064	38.0	34.0	40.0	24.0	41.0
43	34.668400022179554	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	4.0
16	5.0
17	17.0
18	45.0
19	86.0
20	168.0
21	357.0
22	611.0
23	1166.0
24	1854.0
25	2870.0
26	4335.0
27	5962.0
28	8256.0
29	10888.0
30	14009.0
31	17499.0
32	21909.0
33	27519.0
34	34383.0
35	43186.0
36	56307.0
37	80815.0
38	113161.0
39	131695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.67348225981965	20.754174262009883	13.948515702433514	23.62382777573695
2	19.150834852401978	22.38489156275775	35.275199789294206	23.189073795546065
3	19.919148582241107	24.267208217526008	31.981015685105735	23.83262751512715
4	14.277050396113033	16.273210560241758	35.41780741213083	34.031931631514375
5	13.43162804882275	37.46179224685847	34.86955647816353	14.237023226155243
6	33.05811044033353	36.22840092322407	13.816824580494465	16.896664055947934
7	26.50283829023337	32.1466692542817	21.650540280155532	19.6999521753294
8	26.29889032901987	34.9910242103731	18.99453828399537	19.715547176611654
9	24.621561302217263	14.622739591203032	21.518849158216486	39.236849948363215
10	17.16351185566653	26.69483008379714	33.34887057535158	22.79278748518475
11	33.6054949853407	22.21040082618851	23.003493280287227	21.180610908183564
12	21.06070267610222	26.835185095337444	30.445081336595575	21.65903089196476
13	31.21495456656293	20.64587564199422	26.02840369566875	22.1107660957741
14	20.912896719504843	22.74513609237786	27.01036894307478	29.33159824504252
15	23.636650332346807	28.80847259091886	25.008490611809226	22.54638646492511
16	22.498561794326193	27.652189884735613	26.420184783437417	23.42906353750078
17	22.303451000505973	26.555861294593043	26.587397852741603	24.553289852159388
18	23.250240856130915	25.368042030261233	28.75077108617451	22.63094602743334
19	23.51431621117711	24.75117309065201	29.576786320757986	22.157724377412894
20	24.126853205985707	24.378106004422047	29.53104098366337	21.96399980592887
21	23.08614678708318	25.111764175856166	29.907746903525855	21.894342133534796
22	22.08269509346604	25.544092266958696	29.441976198562486	22.93123644101277
23	22.65121952910027	25.656722831774985	30.447680503475954	21.244377135648786
24	22.772860539101867	25.90624285229108	28.163705926793597	23.157190681813454
25	22.750854259514682	25.755837728813326	29.184658677405267	22.308649334266722
26	22.57081863360064	26.46610339832406	29.148616896664052	21.81446107141124
27	21.84131912917513	26.41654594980489	28.74418653007756	22.997948390942426
28	22.04769297947698	26.110883924672677	29.124011450196498	22.717411645653847
29	22.454029401775752	26.09840792364687	28.602445296201058	22.84511737837632
30	22.35248861564906	26.164600040200447	29.85645667708644	21.626454667064053
31	22.550371854141684	25.657069387359037	29.942402461930868	21.850156296568407
32	22.345730781760086	25.671624721889142	28.963382936989262	23.019261559361507
33	21.968158472937475	25.863269959868862	29.526362483278696	22.64220908391497
34	21.66856117052614	25.875399405310617	30.127289866021613	22.32874955814163
35	21.339506643470546	27.46868870298107	29.05712622247482	22.13467843107356
36	22.954628942936157	25.625532829210478	29.127477006037	22.292361221816368
37	21.89451541132682	25.96896941300415	29.125744228116744	23.010770947552277
38	21.938354692709165	24.97816699820484	30.362601107591647	22.720877201494346
39	21.436368929212556	24.817885040581658	30.88070170574658	22.8650443244592
40	20.543988300283484	25.556048434608424	30.67554079998891	23.22442246511918
41	21.61033983240572	24.534749128412706	30.692175468023315	23.162735571158258
42	20.581416303360896	26.269606382167638	30.028174968983272	23.120802345488194
43	20.735113704887127	25.922704242533463	29.594633933336567	23.747548119242843
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	71.0
2	109.0
3	271.0
4	433.0
5	433.0
6	699.5
7	966.0
8	1047.0
9	1128.0
10	1712.5
11	2297.0
12	2297.0
13	4164.0
14	6031.0
15	9022.5
16	12014.0
17	11034.0
18	10054.0
19	10054.0
20	10952.5
21	11851.0
22	9397.5
23	6944.0
24	7721.5
25	8499.0
26	8499.0
27	9356.5
28	10214.0
29	11248.5
30	12283.0
31	13905.5
32	15528.0
33	15528.0
34	17458.5
35	19389.0
36	21885.5
37	24382.0
38	26307.0
39	28232.0
40	28232.0
41	29446.5
42	30661.0
43	33400.5
44	36140.0
45	38095.0
46	40050.0
47	40050.0
48	49660.5
49	59271.0
50	56162.0
51	53053.0
52	49547.0
53	46041.0
54	46041.0
55	41420.0
56	36799.0
57	31829.5
58	26860.0
59	24187.5
60	21515.0
61	21515.0
62	19143.0
63	16771.0
64	14559.0
65	12347.0
66	10615.0
67	8883.0
68	8883.0
69	7742.5
70	6602.0
71	5638.5
72	4675.0
73	3814.5
74	2954.0
75	2954.0
76	2415.0
77	1876.0
78	1450.0
79	1024.0
80	808.5
81	593.0
82	593.0
83	438.0
84	283.0
85	218.5
86	154.0
87	121.0
88	88.0
89	88.0
90	69.0
91	50.0
92	33.0
93	16.0
94	12.0
95	8.0
96	8.0
97	7.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	577108.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.45687419774233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.0367488107496	52.015128992342454
2	7.590291357468081	9.177705794453173
3	2.3259587808337265	4.218605922059962
4	1.0756910324260918	2.601316696920952
5	0.6597885674293646	1.9944377209092863
6	0.43970612515343505	1.5949954735426781
7	0.31686261413380035	1.3409566240458457
8	0.2280841392482628	1.1031403290426056
9	0.1798803738186413	0.9787504617536812
>10	1.0123452897118448	11.473719967953336
>50	0.08285717889947837	3.4070101464767424
>100	0.04603176011351372	4.923536168833084
>500	0.003164683507803923	1.1897052811960784
>1k	0.0020138895049661328	2.049632607253595
>5k	5.753970014188951E-4	1.9313578132165579
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5573	0.9656771349556755	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5531	0.9583994676906229	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3970	0.6879128343394997	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2204	0.3819042536232386	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1154	0.19996257199692258	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1149	0.19909618303679727	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1133	0.19632373836439626	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1116	0.1933780158999702	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1058	0.18332790396251655	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	942	0.16322768008760924	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	675	0.11696250961691745	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	669	0.11592284286476708	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	605	0.10483306417516304	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	604	0.104659786383138	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	595	0.10310028625491242	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	588	0.10188734171073699	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.198333760751887E-4	0.0
10	0.0	0.0	0.0	5.198333760751887E-4	0.0
11	0.0	0.0	0.0	0.0012129445441754403	0.0
12	0.0	0.0	0.0	0.0015595001282255661	0.0
13	0.0	0.0	0.0	0.001732777920250629	0.0
14	1.732777920250629E-4	0.0	0.0	0.002079333504300755	0.0
15	1.732777920250629E-4	0.0	0.0	0.002252611296325818	0.0
16	1.732777920250629E-4	0.0	0.0	0.0027724446724010066	0.0
17	1.732777920250629E-4	0.0	0.0	0.003292278048476195	0.0
18	1.732777920250629E-4	0.0	0.0	0.003465555840501258	0.0
19	1.732777920250629E-4	0.0	0.0	0.004505222592651636	0.0
20	1.732777920250629E-4	0.0	0.0	0.00779750064112783	0.0
21	1.732777920250629E-4	0.0	0.0	0.015941556866305787	0.0
22	1.732777920250629E-4	0.0	0.0	0.032056391524636636	0.0
23	1.732777920250629E-4	0.0	0.0	0.040373725541839656	0.0
24	3.465555840501258E-4	0.0	0.0	0.053023004359669244	0.0
25	3.465555840501258E-4	0.0	0.0	0.06341967188117302	0.0
26	3.465555840501258E-4	0.0	0.0	0.07745517303520312	0.0
27	3.465555840501258E-4	0.0	0.0	0.13931534478815058	0.0
28	3.465555840501258E-4	0.0	0.0	0.2869480235935042	0.0
29	3.465555840501258E-4	0.0	0.0	0.4735682056044969	0.0
30	3.465555840501258E-4	0.0	0.0	0.7099191139266827	0.0
31	3.465555840501258E-4	0.0	0.0	1.0769214774357658	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGTG	25	0.0054941033	29.6	7
TAATACT	90	1.4188117E-10	24.666666	4
GATATAA	40	0.0019300281	23.125002	1
GGTATCA	1905	0.0	23.015747	1
AATACTG	140	0.0	21.142857	5
ATAAGAC	45	0.0038235348	20.555555	3
CCGACCG	55	5.13883E-4	20.181818	9
TAACACT	65	6.894521E-5	19.923077	4
TAAGACT	150	0.0	19.733332	4
TTAACGG	125	4.110916E-10	19.24	35
AACGGCC	155	1.8189894E-12	19.096775	37
GTATTAG	180	0.0	18.5	1
GCTATAC	50	0.0070307404	18.5	3
TAACGGC	130	6.9303496E-10	18.5	36
TACGGCT	50	0.0070307404	18.5	4
ACTTAGC	50	0.0070307404	18.5	8
GTTTAAC	135	1.1477823E-9	17.814816	33
CTAATAC	125	8.552888E-9	17.76	3
TTAGACT	75	2.0650205E-4	17.266666	4
TTTACAC	65	0.0015786455	17.076923	3
>>END_MODULE