FastQCFastQC Report
Wed 25 May 2016
SRR2088597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817946
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT93031.1373611460903286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91231.1153548033733278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66380.8115450164191768No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38970.476437319823069No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG22170.27104478779772745No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA19980.24427040415870974No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA19920.24353685940147637No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG19860.242803314644243No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA18590.22727661728280352No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG16300.19927965904839684No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11370.1390067314957222No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA11260.13766189944079438No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10970.13411643311416646No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA10970.13411643311416646No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG10310.12604744078459948No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10210.12482486618921053No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA9950.1216461722411993No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA9480.11590007164287129No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC9310.11382169483071009No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC8780.10734204947514872No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC8540.10440787044621529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31600.021.7199361
CGAATTA603.724596E-521.58333215
GTTAGAC656.899022E-519.9230753
TACACCG656.899022E-519.9230755
GTATTGG3800.019.4736841
GTCTAAC609.2337135E-418.51
CTACCCT1205.167749E-918.54
CCCGAAT902.1506712E-618.513
GTTCTAT500.007033191618.4999981
TAGGGGT953.6038164E-617.5263164
GTATATA852.7215603E-517.4117641
GTATTAG2600.017.0769211
GTACAAG1201.04007995E-716.9583321
ATAGGGG1201.04007995E-716.9583323
GGGGTTA1251.6558806E-716.2799996
GGGTTAG803.3810726E-416.18757
GCACCGC1851.8189894E-1116.010
ATTGGAC5300.015.7075473
GTTTAGG1659.713403E-1015.696971
TAATACT3100.015.51612954
Produced by FastQC (version 0.11.5)