##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088597_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	817946
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74383883532654	31.0	31.0	34.0	30.0	34.0
2	31.925556210312173	31.0	31.0	34.0	30.0	34.0
3	31.996769957918982	33.0	31.0	34.0	30.0	34.0
4	35.67947639575229	37.0	35.0	37.0	35.0	37.0
5	35.59593053820179	37.0	35.0	37.0	33.0	37.0
6	35.63337433033477	37.0	35.0	37.0	33.0	37.0
7	35.50001100317136	37.0	35.0	37.0	33.0	37.0
8	35.529624205020866	37.0	35.0	37.0	33.0	37.0
9	37.14169517303098	39.0	37.0	39.0	33.0	39.0
10	37.02345998391092	39.0	37.0	39.0	33.0	39.0
11	37.064215730622806	39.0	37.0	39.0	33.0	39.0
12	36.969367170937936	39.0	37.0	39.0	33.0	39.0
13	37.00514092617361	39.0	37.0	39.0	33.0	39.0
14	38.046225545451655	40.0	37.0	41.0	33.0	41.0
15	38.00252217139029	40.0	37.0	41.0	33.0	41.0
16	37.89498695512907	40.0	37.0	41.0	33.0	41.0
17	37.94644511984899	40.0	37.0	41.0	33.0	41.0
18	37.92606846906764	40.0	37.0	41.0	33.0	41.0
19	37.95310326109548	40.0	37.0	41.0	33.0	41.0
20	37.845563154535874	40.0	37.0	41.0	32.0	41.0
21	37.74334980548838	40.0	37.0	41.0	32.0	41.0
22	37.59456981267712	39.0	37.0	41.0	32.0	41.0
23	37.46126516909429	39.0	36.0	41.0	32.0	41.0
24	37.31790729461358	39.0	36.0	41.0	31.0	41.0
25	37.11828898240226	39.0	36.0	41.0	31.0	41.0
26	37.262502659099745	39.0	36.0	41.0	31.0	41.0
27	37.188255703921776	39.0	36.0	41.0	31.0	41.0
28	37.054344663339634	39.0	36.0	41.0	31.0	41.0
29	36.9169333916909	39.0	35.0	41.0	31.0	41.0
30	36.77839123854142	39.0	35.0	41.0	30.0	41.0
31	36.591537827680554	39.0	35.0	40.0	30.0	41.0
32	36.45348837209303	39.0	35.0	40.0	30.0	41.0
33	36.247286495685536	38.0	35.0	40.0	30.0	41.0
34	36.09716044824475	38.0	35.0	40.0	30.0	41.0
35	35.90940844505627	38.0	35.0	40.0	29.0	41.0
36	35.759449890335055	38.0	35.0	40.0	27.0	41.0
37	35.58536138082465	38.0	35.0	40.0	27.0	41.0
38	35.470336672592076	38.0	35.0	40.0	26.0	41.0
39	35.315569731987196	38.0	34.0	40.0	25.0	41.0
40	35.11651135894056	38.0	34.0	40.0	24.0	41.0
41	34.97058852295873	38.0	34.0	40.0	24.0	41.0
42	34.731551960642875	38.0	34.0	40.0	23.0	41.0
43	34.371024493059444	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	7.0
15	3.0
16	7.0
17	19.0
18	65.0
19	123.0
20	271.0
21	543.0
22	994.0
23	1709.0
24	2837.0
25	4375.0
26	6403.0
27	9323.0
28	12476.0
29	16325.0
30	20830.0
31	25801.0
32	31643.0
33	39442.0
34	49427.0
35	61127.0
36	79459.0
37	114548.0
38	155236.0
39	184951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.728793832355684	21.146750518982916	13.942974230572677	23.181481418088726
2	19.048445740916883	22.864223310585295	35.432167893724035	22.655163054773787
3	19.923686893755825	24.55761138265851	32.42854662777249	23.090155095813174
4	13.882456788100924	16.238969320713103	35.963987842718225	33.91458604846775
5	13.015162370132014	38.00299775290789	35.02370572140459	13.958134155555502
6	32.438327224535605	36.65193056754358	13.682443584295296	17.22729862362552
7	25.89364090049954	32.84813422890998	21.781266733989774	19.476958136600704
8	26.514342022578507	34.8040090666132	18.998197925046398	19.683450985761898
9	24.28485499042724	14.69118988295071	22.20403302907527	38.81992209754678
10	16.97459245475863	27.077826653593267	33.550136561582306	22.3974443300658
11	32.77367943605079	22.39157597200793	23.581630083159524	21.253114508781753
12	20.91164942428962	27.25901220862991	30.828807769706067	21.000530597374397
13	31.116846344379702	20.84550813867908	26.588674557978152	21.448970958963063
14	20.363446975717224	23.223904756548723	27.793399564274413	28.619248703459643
15	22.982201759040326	29.089450892846223	25.958559611514698	21.969787736598747
16	21.69226330344546	28.193303714426136	27.148002435368596	22.96643054675981
17	21.581253530184146	26.76851528095987	27.256567059439128	24.393664129416855
18	22.773508275607437	25.554865480117268	29.59412968582278	22.077496558452513
19	22.723015944817874	24.88709523611583	30.6710956469009	21.718793172165398
20	23.609993813772547	24.443545172908728	30.669995329765044	21.276465683553685
21	22.419939702620955	25.531269790426265	31.016595227557808	21.032195279394973
22	21.06679414044448	25.994014274780973	30.55116107909324	22.388030505681304
23	21.988859900286815	26.04524015032777	31.643898252451873	20.32200169693354
24	22.13324596000225	26.187058803392887	29.14336643250288	22.53632880410198
25	22.118819579776662	26.202585500754328	30.199181853080763	21.479413066388243
26	21.692630075824077	26.9993373645693	30.257620918740358	21.05041164086627
27	20.846119425976774	27.020732419988608	29.785707124920226	22.347441029114393
28	21.17181329818839	26.60701317690899	30.158592376513855	22.06258114838877
29	21.58736640316109	26.6454020192042	29.527499370374084	22.239732207260626
30	21.582965134617687	26.606524147070832	31.091294535336072	20.7192161829754
31	21.964530665838577	26.335479359273105	30.846535101339207	20.85345487354911
32	21.50887711413712	26.22459184347133	29.995500925488965	22.271030116902583
33	21.221572084220718	26.341347717330972	30.617180107244245	21.819900091204065
34	20.745868309154883	26.68379086149941	31.072344629107544	21.497996200238155
35	20.41589542585941	28.46813848346957	29.829842067813768	21.286124022857255
36	22.36981414421001	26.34758284776746	29.806857665420456	21.475745342602078
37	21.34859758468163	26.696750152210534	29.901973968941714	22.05267829416612
38	21.26032769889455	25.439943468150712	31.20254882351647	22.097180009438276
39	20.655275531636562	25.5335926821575	31.809923882505693	22.001207903700244
40	19.840429563809835	26.29513439762527	31.357326767292705	22.507109271272185
41	21.022170167712783	25.032826127886192	31.41417648597829	22.530827218422733
42	19.69506544441809	27.141889562391647	30.689312008372188	22.473732984818067
43	20.21490416237747	26.759468228953992	29.886325014120736	23.139302594547807
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	45.0
1	114.5
2	184.0
3	470.0
4	756.0
5	756.0
6	1151.0
7	1546.0
8	1707.5
9	1869.0
10	2841.5
11	3814.0
12	3814.0
13	6972.0
14	10130.0
15	15138.5
16	20147.0
17	18487.5
18	16828.0
19	16828.0
20	18458.5
21	20089.0
22	16112.5
23	12136.0
24	13344.0
25	14552.0
26	14552.0
27	15736.0
28	16920.0
29	18153.0
30	19386.0
31	21766.0
32	24146.0
33	24146.0
34	27036.5
35	29927.0
36	33154.5
37	36382.0
38	38444.5
39	40507.0
40	40507.0
41	42038.5
42	43570.0
43	46769.0
44	49968.0
45	52321.0
46	54674.0
47	54674.0
48	69504.5
49	84335.0
50	79448.5
51	74562.0
52	68453.0
53	62344.0
54	62344.0
55	55439.0
56	48534.0
57	41258.0
58	33982.0
59	30177.0
60	26372.0
61	26372.0
62	23338.5
63	20305.0
64	17983.5
65	15662.0
66	13351.5
67	11041.0
68	11041.0
69	9631.0
70	8221.0
71	6982.5
72	5744.0
73	4807.0
74	3870.0
75	3870.0
76	3093.5
77	2317.0
78	1843.0
79	1369.0
80	1088.5
81	808.0
82	808.0
83	613.0
84	418.0
85	323.5
86	229.0
87	176.5
88	124.0
89	124.0
90	103.0
91	82.0
92	57.0
93	32.0
94	21.5
95	11.0
96	11.0
97	7.0
98	3.0
99	4.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	817946.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.393209679062686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.96948234001155	48.480950436007475
2	7.843634043160318	8.846553984835479
3	2.169417015244499	3.6702116586602807
4	1.034945373084172	2.3345556572284587
5	0.6156946328289151	1.736049826369726
6	0.390182410795958	1.3202183103059177
7	0.31067634247589954	1.2264025286497384
8	0.21426409543816102	0.966643204859113
9	0.1605150878929464	0.8146764907380094
>10	1.1065036542535336	11.841756370622724
>50	0.110724395842956	4.3444894816168
>100	0.0669996104810607	7.2289870419930375
>500	0.0034804992450103442	1.4042358543298057
>1k	0.002827905636570905	2.7095974098398488
>5k	6.525936084394396E-4	3.0746717439435876
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9303	1.1373611460903286	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9123	1.1153548033733278	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6638	0.8115450164191768	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3897	0.476437319823069	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2217	0.27104478779772745	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1998	0.24427040415870974	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1992	0.24353685940147637	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1986	0.242803314644243	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1859	0.22727661728280352	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1630	0.19927965904839684	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1137	0.1390067314957222	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1126	0.13766189944079438	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1097	0.13411643311416646	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1097	0.13411643311416646	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1031	0.12604744078459948	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1021	0.12482486618921053	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	995	0.1216461722411993	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	948	0.11590007164287129	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	931	0.11382169483071009	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	878	0.10734204947514872	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	854	0.10440787044621529	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.2225745953889377E-4	0.0	0.0	0.0	0.0
9	1.2225745953889377E-4	0.0	0.0	1.2225745953889377E-4	0.0
10	1.2225745953889377E-4	0.0	0.0	1.2225745953889377E-4	0.0
11	1.2225745953889377E-4	0.0	0.0	7.335447572333626E-4	0.0
12	1.2225745953889377E-4	0.0	0.0	0.0011003171358500438	0.0
13	1.2225745953889377E-4	0.0	0.0	0.0011003171358500438	0.0
14	1.2225745953889377E-4	0.0	0.0	0.0014670895144667253	0.0
15	1.2225745953889377E-4	0.0	0.0	0.0017116044335445128	0.0
16	1.2225745953889377E-4	0.0	0.0	0.002689664109855663	0.0
17	1.2225745953889377E-4	0.0	0.0	0.005379328219711326	0.0
18	1.2225745953889377E-4	0.0	0.0	0.0061128729769446884	0.0
19	1.2225745953889377E-4	0.0	0.0	0.00745770503187252	0.0
20	1.2225745953889377E-4	0.0	0.0	0.011614458656194909	0.0
21	1.2225745953889377E-4	0.0	0.0	0.018338618930834064	0.0
22	1.2225745953889377E-4	0.0	0.0	0.03178693948011238	0.0
23	1.2225745953889377E-4	0.0	0.0	0.040344961647834944	0.0
24	1.2225745953889377E-4	0.0	0.0	0.05354876727803547	0.0
25	1.2225745953889377E-4	0.0	0.0	0.06174001706714135	0.0
26	1.2225745953889377E-4	0.0	0.0	0.07213190112794732	0.0
27	1.2225745953889377E-4	0.0	0.0	0.12531389602736612	0.0
28	1.2225745953889377E-4	0.0	0.0	0.24720458318764318	0.0
29	1.2225745953889377E-4	0.0	0.0	0.4100515192934497	0.0
30	1.2225745953889377E-4	0.0	0.0	0.6425852073364257	0.0
31	1.2225745953889377E-4	0.0	0.0	0.9811161127996225	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3160	0.0	21.719936	1
CGAATTA	60	3.724596E-5	21.583332	15
GTTAGAC	65	6.899022E-5	19.923075	3
TACACCG	65	6.899022E-5	19.923075	5
GTATTGG	380	0.0	19.473684	1
GTCTAAC	60	9.2337135E-4	18.5	1
CTACCCT	120	5.167749E-9	18.5	4
CCCGAAT	90	2.1506712E-6	18.5	13
GTTCTAT	50	0.0070331916	18.499998	1
TAGGGGT	95	3.6038164E-6	17.526316	4
GTATATA	85	2.7215603E-5	17.411764	1
GTATTAG	260	0.0	17.076921	1
GTACAAG	120	1.04007995E-7	16.958332	1
ATAGGGG	120	1.04007995E-7	16.958332	3
GGGGTTA	125	1.6558806E-7	16.279999	6
GGGTTAG	80	3.3810726E-4	16.1875	7
GCACCGC	185	1.8189894E-11	16.0	10
ATTGGAC	530	0.0	15.707547	3
GTTTAGG	165	9.713403E-10	15.69697	1
TAATACT	310	0.0	15.5161295	4
>>END_MODULE