FastQCFastQC Report
Wed 25 May 2016
SRR2088596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872326
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT251411.3427682999648565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT248871.329202286353979No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT186310.995072439308112No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101020.5395427932956119No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG58900.3145819691656261No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA58490.31239217956701987No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG55460.2962091003382958No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA55270.29519431979260025No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA53780.28723630393425076No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG45170.24125072236352002No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA31880.1702694936672353No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA30060.16054896422951986No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28460.15200344384471506No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28310.15120230130863963No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA27220.14538066554649137No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG27000.1442056564935807No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25710.13731583068333186No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC25610.13678173565928156No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC24930.13314988949573953No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA24860.1327760229789043No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC21240.11344178310828348No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG21150.11296109758663822No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC20670.11039744147119679No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG20380.10884856590145091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83700.022.2574671
GTATTGG12800.018.0664061
ATTAGAG2900.017.8620683
CTAATAC3500.017.4428563
GTATTAG3450.017.159421
ATTGGAC14000.016.7821433
AGTTCCG2000.016.65000232
GTCGAAT904.4483593E-516.4444458
TTGGACC17150.016.3965024
TATTGGA14600.016.0924662
TATACCG1059.349298E-615.8571425
TAATACT2950.015.6779664
TGGACCC17050.015.6246345
CTCTAAT3150.015.2698421
TTAGAGT855.36639E-415.2352944
GTCGGGT2201.8189894E-1215.13636424
TAGCGAA2700.015.07407510
GTCTAAC1353.9757288E-715.0740751
TATACTG1752.240995E-914.7999995
GTATCAA126150.014.7530732
Produced by FastQC (version 0.11.5)