##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088596_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1872326
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.677164126332702	31.0	31.0	34.0	30.0	34.0
2	31.85183456299811	31.0	31.0	34.0	30.0	34.0
3	31.913793858548136	31.0	31.0	34.0	30.0	34.0
4	35.60291316789918	37.0	35.0	37.0	33.0	37.0
5	35.52776065706506	37.0	35.0	37.0	33.0	37.0
6	35.573705647413966	37.0	35.0	37.0	33.0	37.0
7	35.427504077815506	37.0	35.0	37.0	33.0	37.0
8	35.47077966123421	37.0	35.0	37.0	33.0	37.0
9	37.059773244616586	39.0	37.0	39.0	33.0	39.0
10	36.94046923452433	39.0	37.0	39.0	33.0	39.0
11	36.980002948204536	39.0	37.0	39.0	33.0	39.0
12	36.87743801026103	39.0	37.0	39.0	32.0	39.0
13	36.92264968814192	39.0	37.0	39.0	33.0	39.0
14	37.91551204224051	40.0	37.0	41.0	33.0	41.0
15	37.879068068274435	40.0	37.0	41.0	32.0	41.0
16	37.75971545553499	40.0	37.0	41.0	32.0	41.0
17	37.83541915243392	40.0	37.0	41.0	32.0	41.0
18	37.82287539669908	40.0	37.0	41.0	32.0	41.0
19	37.85244823818074	40.0	37.0	41.0	32.0	41.0
20	37.73906200095496	40.0	37.0	41.0	32.0	41.0
21	37.636305322897826	40.0	37.0	41.0	32.0	41.0
22	37.48457854027557	39.0	36.0	41.0	32.0	41.0
23	37.34112435548083	39.0	36.0	41.0	31.0	41.0
24	37.194596453822676	39.0	36.0	41.0	31.0	41.0
25	36.99270853473166	39.0	36.0	41.0	31.0	41.0
26	37.1215301181525	39.0	36.0	41.0	31.0	41.0
27	37.04890494497219	39.0	36.0	41.0	31.0	41.0
28	36.91226688087438	39.0	35.0	41.0	31.0	41.0
29	36.75167732542303	39.0	35.0	41.0	30.0	41.0
30	36.59672193838039	39.0	35.0	40.0	30.0	41.0
31	36.372250345292436	38.0	35.0	40.0	30.0	41.0
32	36.23664949373133	38.0	35.0	40.0	30.0	41.0
33	36.00590228411078	38.0	35.0	40.0	30.0	41.0
34	35.84037929292228	38.0	35.0	40.0	29.0	41.0
35	35.62689456857406	38.0	35.0	40.0	27.0	41.0
36	35.45834539497929	38.0	34.0	40.0	26.0	41.0
37	35.26562468288108	38.0	34.0	40.0	25.0	41.0
38	35.14189996827476	38.0	34.0	40.0	25.0	41.0
39	34.96483144495136	38.0	34.0	40.0	24.0	41.0
40	34.74808019543605	38.0	33.0	40.0	23.0	41.0
41	34.58310411755218	38.0	33.0	40.0	22.0	41.0
42	34.31011586657452	38.0	33.0	40.0	21.0	41.0
43	33.92514177552413	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	2.0
14	6.0
15	14.0
16	24.0
17	54.0
18	127.0
19	332.0
20	711.0
21	1426.0
22	2513.0
23	4585.0
24	7448.0
25	11274.0
26	16620.0
27	23451.0
28	31626.0
29	40699.0
30	51180.0
31	62676.0
32	76363.0
33	94075.0
34	115946.0
35	142370.0
36	184732.0
37	267422.0
38	347694.0
39	388952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.054375146208514	21.146317468218676	14.162223886224941	22.63708349934787
2	19.236874347736453	23.039043414448123	35.21496790623001	22.509114331585415
3	20.16790879366093	24.607466862074233	32.727954426739785	22.496669917525047
4	13.940841498756093	16.080479574603995	35.704412586269704	34.27426634037021
5	12.913296081985722	38.26117887590089	34.617101936308096	14.208423105805293
6	32.42672483317542	36.34388455856512	13.263715827265123	17.965674780994334
7	25.671010283465595	33.135575749094976	21.457214181718353	19.736199785721077
8	26.966083897782756	34.394597949288745	18.70550320830881	19.933814944619687
9	24.014033880851944	14.626833147646295	22.30103091021542	39.058102061286334
10	17.192251776667096	26.858997845460674	32.971501757706726	22.977248620165504
11	32.5231823945189	22.2657806386281	23.694431418460248	21.516605548392747
12	21.25383079656	27.155046717291754	30.78507695775202	20.80604552839623
13	31.98043503107899	20.544819652133228	26.670942987492563	20.803802329295216
14	20.360289821323853	23.31452962785327	27.962812031665425	28.362368519157457
15	22.927417554421613	28.963332240218854	26.093212399977357	22.016037805382183
16	21.58384811192068	27.96409385972315	27.347481154457075	23.104576873899095
17	21.63015415050584	26.37104863148832	27.14778302496467	24.85101419304117
18	23.21566863890156	24.72934734656251	29.935331774487988	22.11965224004794
19	22.87694557464886	24.211862677760177	31.180841370573287	21.730350377017675
20	24.019214602585233	23.603047759845243	31.086306551316383	21.291431086253144
21	22.66031663289406	24.738747418985795	31.68059408457715	20.920341863542994
22	21.078060124145047	25.169014370360713	31.20359381859783	22.549331686896405
23	22.04087322400052	25.40412299994766	32.49674469082841	20.058259085223405
24	22.221824618148762	25.412988977346895	29.663477407246386	22.701708997257956
25	22.04530621270014	25.683721745037992	30.664745348833485	21.606226693428386
26	21.84550126420292	26.365867909755032	30.76066881515292	21.02796201088913
27	20.63999538541899	26.51220994634481	30.37814995892809	22.4696447093081
28	21.147599296276397	26.08146230944825	30.6001732604258	22.170765133849553
29	21.51089073163541	26.068804257378254	29.809552396324147	22.610752614662193
30	21.377313566120428	26.254989782762188	31.650791582235144	20.716905068882234
31	22.156130930190578	25.727731175019734	31.521433767410162	20.594704127379526
32	21.73830839287603	25.570386780934516	30.31080057639535	22.380504249794107
33	21.222639647155464	25.776654279222743	31.030173164288698	21.970532909333095
34	20.566664138616886	26.345839346353145	31.540340731261544	21.547155783768428
35	20.16785538415853	28.58839753333554	30.2390716146654	21.004675467840535
36	22.433860342696732	25.82146485174056	30.306367587695732	21.438307217866974
37	21.383509068399416	26.707154630123174	30.024685872011602	21.88465042946581
38	21.251427368951774	25.049056627959022	31.55107604124495	22.14843996184425
39	20.581618799290293	24.92343747830239	32.35312653886129	22.141817183546028
40	19.49040925565313	25.96657846977503	31.701584019022327	22.841428255549516
41	21.10123984818883	24.685978830609624	31.77251183821621	22.440269482985332
42	19.533403905089177	26.89029581387002	31.072847356710316	22.503452924330485
43	19.998654080539392	26.824708944916644	30.07932379297195	23.097313181572012
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	94.0
1	239.5
2	385.0
3	1105.0
4	1825.0
5	1825.0
6	2845.0
7	3865.0
8	4305.0
9	4745.0
10	7133.0
11	9521.0
12	9521.0
13	17721.5
14	25922.0
15	39329.5
16	52737.0
17	48163.5
18	43590.0
19	43590.0
20	47085.5
21	50581.0
22	38370.5
23	26160.0
24	28529.5
25	30899.0
26	30899.0
27	33106.5
28	35314.0
29	38168.0
30	41022.0
31	44290.5
32	47559.0
33	47559.0
34	52358.0
35	57157.0
36	64263.0
37	71369.0
38	76527.5
39	81686.0
40	81686.0
41	85519.0
42	89352.0
43	98040.0
44	106728.0
45	116814.5
46	126901.0
47	126901.0
48	170028.0
49	213155.0
50	200332.0
51	187509.0
52	171516.5
53	155524.0
54	155524.0
55	136328.5
56	117133.0
57	98230.5
58	79328.0
59	69664.0
60	60000.0
61	60000.0
62	52796.5
63	45593.0
64	39514.5
65	33436.0
66	29149.0
67	24862.0
68	24862.0
69	21237.5
70	17613.0
71	14836.0
72	12059.0
73	9980.0
74	7901.0
75	7901.0
76	6325.0
77	4749.0
78	3758.0
79	2767.0
80	2154.0
81	1541.0
82	1541.0
83	1168.5
84	796.0
85	628.5
86	461.0
87	351.0
88	241.0
89	241.0
90	194.0
91	147.0
92	105.0
93	63.0
94	42.0
95	21.0
96	21.0
97	16.5
98	12.0
99	7.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1872326.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.3988753252535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.13476381583324	37.645617908347354
2	10.292000155472019	9.550744641224716
3	3.2171111826089156	4.478110220080498
4	1.4066661321827418	2.610709059656144
5	0.8272056230648858	1.9190705286468137
6	0.5160633460696806	1.4366855312532156
7	0.37715593169430933	1.224972773700486
8	0.26517199590089524	0.9842945902043416
9	0.21354651995126087	0.8917486519824281
>10	1.4826584041577184	13.30736291435461
>50	0.15156415238263454	4.8593589451705315
>100	0.1025508359550639	8.907807221376945
>500	0.007291794852805193	2.250922047388437
>1k	0.005208424894860852	4.188968400543801
>5k	5.787138772067613E-4	1.5138973258548465
>10k+	4.6297110176540904E-4	4.229729240214742
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	25141	1.3427682999648565	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24887	1.329202286353979	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18631	0.995072439308112	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10102	0.5395427932956119	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5890	0.3145819691656261	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5849	0.31239217956701987	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5546	0.2962091003382958	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5527	0.29519431979260025	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5378	0.28723630393425076	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4517	0.24125072236352002	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3188	0.1702694936672353	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3006	0.16054896422951986	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2846	0.15200344384471506	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2831	0.15120230130863963	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2722	0.14538066554649137	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2700	0.1442056564935807	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2571	0.13731583068333186	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2561	0.13678173565928156	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2493	0.13314988949573953	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2486	0.1327760229789043	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2124	0.11344178310828348	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2115	0.11296109758663822	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2067	0.11039744147119679	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2038	0.10884856590145091	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	5.3409502405029894E-5	0.0	5.3409502405029894E-5	0.0	5.3409502405029894E-5
7	5.3409502405029894E-5	0.0	5.3409502405029894E-5	5.3409502405029894E-5	5.3409502405029894E-5
8	5.3409502405029894E-5	0.0	5.3409502405029894E-5	5.3409502405029894E-5	5.3409502405029894E-5
9	1.0681900481005979E-4	0.0	5.3409502405029894E-5	1.0681900481005979E-4	5.3409502405029894E-5
10	1.0681900481005979E-4	0.0	1.0681900481005979E-4	3.2045701443017934E-4	5.3409502405029894E-5
11	1.0681900481005979E-4	0.0	1.6022850721508967E-4	8.545520384804783E-4	5.3409502405029894E-5
12	1.0681900481005979E-4	0.0	1.6022850721508967E-4	0.0011750090529106577	5.3409502405029894E-5
13	1.0681900481005979E-4	0.0	1.6022850721508967E-4	0.0016022850721508968	5.3409502405029894E-5
14	2.1363800962011958E-4	0.0	1.6022850721508967E-4	0.0019227420865810761	5.3409502405029894E-5
15	2.1363800962011958E-4	0.0	1.6022850721508967E-4	0.003097751139491734	5.3409502405029894E-5
16	2.6704751202514946E-4	0.0	1.6022850721508967E-4	0.00523413123569293	5.3409502405029894E-5
17	2.6704751202514946E-4	0.0	1.6022850721508967E-4	0.007103463819868976	5.3409502405029894E-5
18	3.2045701443017934E-4	0.0	1.6022850721508967E-4	0.008171653867969574	5.3409502405029894E-5
19	3.2045701443017934E-4	0.0	1.6022850721508967E-4	0.01009439595455065	5.3409502405029894E-5
20	3.2045701443017934E-4	0.0	1.6022850721508967E-4	0.015595574702268728	5.3409502405029894E-5
21	3.2045701443017934E-4	0.0	1.6022850721508967E-4	0.026758160704919976	5.3409502405029894E-5
22	3.2045701443017934E-4	0.0	1.6022850721508967E-4	0.04422306799136475	5.3409502405029894E-5
23	3.2045701443017934E-4	0.0	1.6022850721508967E-4	0.05554588250123109	5.3409502405029894E-5
24	3.738665168352093E-4	0.0	1.6022850721508967E-4	0.07204941874438532	5.3409502405029894E-5
25	3.738665168352093E-4	0.0	1.6022850721508967E-4	0.08225063370374604	5.3409502405029894E-5
26	4.2727601924023916E-4	0.0	1.6022850721508967E-4	0.09592346631943369	5.3409502405029894E-5
27	4.2727601924023916E-4	0.0	1.6022850721508967E-4	0.157237575080408	5.3409502405029894E-5
28	4.2727601924023916E-4	0.0	1.6022850721508967E-4	0.3004818605306982	5.3409502405029894E-5
29	4.2727601924023916E-4	0.0	1.6022850721508967E-4	0.4911537841166549	5.3409502405029894E-5
30	4.2727601924023916E-4	0.0	1.6022850721508967E-4	0.752326250877251	5.3409502405029894E-5
31	4.2727601924023916E-4	0.0	1.6022850721508967E-4	1.1209052269743625	5.3409502405029894E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8370	0.0	22.257467	1
GTATTGG	1280	0.0	18.066406	1
ATTAGAG	290	0.0	17.862068	3
CTAATAC	350	0.0	17.442856	3
GTATTAG	345	0.0	17.15942	1
ATTGGAC	1400	0.0	16.782143	3
AGTTCCG	200	0.0	16.650002	32
GTCGAAT	90	4.4483593E-5	16.444445	8
TTGGACC	1715	0.0	16.396502	4
TATTGGA	1460	0.0	16.092466	2
TATACCG	105	9.349298E-6	15.857142	5
TAATACT	295	0.0	15.677966	4
TGGACCC	1705	0.0	15.624634	5
CTCTAAT	315	0.0	15.269842	1
TTAGAGT	85	5.36639E-4	15.235294	4
GTCGGGT	220	1.8189894E-12	15.136364	24
TAGCGAA	270	0.0	15.074075	10
GTCTAAC	135	3.9757288E-7	15.074075	1
TATACTG	175	2.240995E-9	14.799999	5
GTATCAA	12615	0.0	14.753073	2
>>END_MODULE