FastQCFastQC Report
Wed 25 May 2016
SRR2088595_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088595_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4077129
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT397400.9747054851587968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT391310.9597685037682152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT294730.722886128940242No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156190.38308819760179286No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG99930.24509894094594506No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA92910.2278809426927625No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA91840.22525654694761926No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG88970.21821727985550615No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA86590.2123798388522904No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG73440.1801267509563715No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA55520.13617425399098237No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA50310.12339565414780843No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG44720.10968502590916304No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44410.10892468695496266No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43280.10615312883158713No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA43230.10603049351639351No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC42410.10401927434721835No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC41660.10217974461931421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122650.024.3147181
GTATTGG19550.018.074171
ACCGTTA5950.015.8571428
GTATCAA189250.015.7384412
ATTGGAC22950.015.4771233
TTGGACC27800.015.3723024
CTCTAAT8200.015.1158541
TAATACT10800.015.0740754
CTAATAC10150.014.5812813
TGGACCC27800.014.5737415
GTATTAG10300.014.3689331
ATTAGAG9350.014.2459893
GGGTTAG3550.014.0704237
GGACCCT28150.014.0639436
TAAACGT2650.013.9622644
CGTTATT4000.013.87500110
TCTAATA9750.013.8512822
CCGTTTA7000.013.74285827
CGGGTAA3700.013.50000126
CCGTTAT7400.013.5000019
Produced by FastQC (version 0.11.5)