FastQCFastQC Report
Wed 25 May 2016
SRR2088594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3556139
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT358871.009156278761882No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT343800.9667788576318304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT253750.7135547851194793No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141490.3978753361440596No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG80630.22673466925786645No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA74500.2094968728725171No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA71130.20002030291841796No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG71130.20002030291841796No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA67050.18854718558526537No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG55570.1562649828929634No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA41390.11639027608313399No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38660.10871341080874511No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38110.10716678959961914No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA37900.10657626150158922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119100.021.7464311
GTATTAG8800.016.18751
CTAATAC9150.015.3661193
TAATACT9350.015.0374344
AATACTG9350.015.0374345
TACGTGC1853.0559022E-1015.08
GTATTGG17550.014.7578341
GTATCAA175650.014.7031032
TCTAATA9650.014.5699472
TTACGCA908.280022E-414.3888894
CTCTAAT8100.014.3888891
TTGGACC24150.014.1718434
ATTAGAG7950.013.9622633
TTAACGG5450.013.91743135
TGGACCC24550.013.86558155
TATACCG2551.8189894E-1213.7843135
TTATGCG4200.013.6547634
TAAACGT1907.1413524E-913.6315794
ACCGTTA5600.013.5446438
GGTAAGA5800.013.3965512
Produced by FastQC (version 0.11.5)