##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088594_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3556139
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.586378653927756	31.0	31.0	34.0	30.0	34.0
2	31.77667014703306	31.0	31.0	34.0	30.0	34.0
3	31.851462217871685	31.0	31.0	34.0	30.0	34.0
4	35.5573502610556	37.0	35.0	37.0	33.0	37.0
5	35.46187311575841	37.0	35.0	37.0	33.0	37.0
6	35.49664903424754	37.0	35.0	37.0	33.0	37.0
7	35.37157012141539	37.0	35.0	37.0	33.0	37.0
8	35.391466419057295	37.0	35.0	37.0	33.0	37.0
9	36.97662211741442	39.0	37.0	39.0	33.0	39.0
10	36.82655824195848	39.0	37.0	39.0	32.0	39.0
11	36.874020391216426	39.0	37.0	39.0	33.0	39.0
12	36.77331397900926	39.0	37.0	39.0	32.0	39.0
13	36.82051573349636	39.0	37.0	39.0	32.0	39.0
14	37.839296214236846	40.0	37.0	41.0	32.0	41.0
15	37.79618232020739	40.0	37.0	41.0	32.0	41.0
16	37.685302796094305	39.0	37.0	41.0	32.0	41.0
17	37.708366292768645	39.0	37.0	41.0	32.0	41.0
18	37.68081843819941	39.0	37.0	41.0	32.0	41.0
19	37.70642289291842	40.0	37.0	41.0	32.0	41.0
20	37.58004959873616	39.0	37.0	41.0	32.0	41.0
21	37.47444180331534	39.0	36.0	41.0	32.0	41.0
22	37.32252282602002	39.0	36.0	41.0	31.0	41.0
23	37.193035480334146	39.0	36.0	41.0	31.0	41.0
24	37.05623542836768	39.0	36.0	41.0	31.0	41.0
25	36.86197980450145	39.0	36.0	40.0	30.0	41.0
26	37.01354334012253	39.0	36.0	40.0	31.0	41.0
27	36.9420388235668	39.0	36.0	41.0	31.0	41.0
28	36.82018335053832	39.0	35.0	41.0	30.0	41.0
29	36.67355297416665	39.0	35.0	40.0	30.0	41.0
30	36.5649346102613	39.0	35.0	40.0	30.0	41.0
31	36.38781020651892	39.0	35.0	40.0	30.0	41.0
32	36.26978107436183	38.0	35.0	40.0	30.0	41.0
33	36.0735421196978	38.0	35.0	40.0	29.0	41.0
34	35.932431493819564	38.0	35.0	40.0	29.0	41.0
35	35.75798330717669	38.0	35.0	40.0	27.0	41.0
36	35.62203080363282	38.0	35.0	40.0	27.0	41.0
37	35.46506365471091	38.0	34.0	40.0	26.0	41.0
38	35.38331713130449	38.0	34.0	40.0	26.0	41.0
39	35.247046304995386	38.0	34.0	40.0	25.0	41.0
40	35.06702353310711	38.0	34.0	40.0	24.0	41.0
41	34.94800962504559	38.0	34.0	40.0	24.0	41.0
42	34.74175925069296	38.0	33.0	40.0	23.0	41.0
43	34.38727788761913	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	4.0
14	7.0
15	17.0
16	53.0
17	98.0
18	269.0
19	667.0
20	1394.0
21	2776.0
22	5075.0
23	8701.0
24	14085.0
25	21632.0
26	31356.0
27	43536.0
28	58946.0
29	76500.0
30	97081.0
31	119292.0
32	145807.0
33	178741.0
34	219706.0
35	272181.0
36	353535.0
37	499343.0
38	697204.0
39	708129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.447001931026875	21.37157743271565	13.751009170338953	23.430411465918514
2	19.367437549544604	23.07738814483911	34.5166766540903	23.038497651525994
3	20.353253908241495	24.587171648802254	31.90263372719683	23.156940715759422
4	14.325958574735125	16.423148813924314	35.768483740371224	33.48240887096933
5	13.308928587999514	38.131664707144466	34.69330079617248	13.866105908683545
6	32.88605985311598	36.77873671417231	13.747578483293257	16.587624949418455
7	26.544434849143972	32.369572730424764	21.616955917639892	19.46903650279137
8	26.89152476885746	34.639422137323656	18.975664337080186	19.4933887567387
9	24.67847854091193	14.394150509864772	21.518478327196995	39.4088926220263
10	16.799005888127542	27.08631468005047	33.581561350667116	22.53311808115487
11	33.660017226548234	22.2525328734338	23.103427621923665	20.984022278094304
12	20.93087474927161	26.81132542906787	30.82140490008968	21.43639492157084
13	31.330861926375768	20.65436699746551	26.138685805026178	21.87608527113254
14	20.70197481032097	22.567228108912506	27.384925054954262	29.345872025812263
15	23.43021462321917	28.94588766074667	25.11282601720574	22.51107169882842
16	22.389422910634256	27.898431416769704	26.431475260106534	23.280670412489503
17	22.095002473187915	26.45610871791007	26.981622484385454	24.46726632451656
18	23.08171868422466	25.356882844005817	29.060281389450747	22.50111708231877
19	23.15561905763526	24.764892485923635	29.927289118901147	22.152199337539955
20	23.92459912281269	24.433746824856957	29.84351849013776	21.79813556219259
21	22.95900132137692	25.217124527472066	30.129530932283583	21.69434321886743
22	21.92661760409253	25.687325495431985	29.689953064264362	22.696103836211126
23	22.630077170774257	25.733021122065253	30.6121048699165	21.02479683724399
24	22.7099390659364	25.86808333420038	28.41323131632369	23.008746283539537
25	22.73215417057657	25.77399252391428	29.277989414924445	22.215863890584707
26	22.388691780608124	26.494521164667635	29.43239845236646	21.68438860235778
27	21.755280094506993	26.474358848177758	28.986099812183948	22.784261245131308
28	21.95004188531438	26.227321260501906	29.321407290322455	22.501229563861255
29	22.307255143851236	26.191074083437123	28.842067197035888	22.659603575675753
30	22.18819343113416	26.21666363435175	30.147106173296372	21.448036761217715
31	22.52712843901771	25.94479574617303	30.068369093559056	21.45970672125021
32	22.194886082911832	25.831667434821863	29.17180683882154	22.80163964344476
33	21.93972170379167	25.898425230284865	29.74996196717845	22.411891098745016
34	21.600730455136876	26.218350857488982	30.000036556501307	22.18088213087284
35	21.22318615779642	27.806899561575065	29.1775152771025	21.792399003526015
36	22.849050613600873	25.918868750630953	29.1087046934892	22.123375942278972
37	21.92566151098143	26.24517770537091	29.25144377089872	22.577717012748938
38	21.856485362355073	25.151463427048267	30.368722932371316	22.623328278225344
39	21.291293731769205	25.145108219898045	30.957760649963344	22.60583739836941
40	20.672195321948887	25.779026072940344	30.613539009583146	22.93523959552762
41	21.644260812077366	24.65494177814759	30.69351338628777	23.00728402348727
42	20.466719664220097	26.47627103439995	30.09010615164368	22.96690314973627
43	20.866872751599416	26.170461840777314	29.488695464378644	23.473969943244626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	210.0
1	397.0
2	584.0
3	1567.5
4	2551.0
5	2551.0
6	4121.0
7	5691.0
8	6243.0
9	6795.0
10	10105.0
11	13415.0
12	13415.0
13	25014.5
14	36614.0
15	55911.0
16	75208.0
17	69609.5
18	64011.0
19	64011.0
20	70367.5
21	76724.0
22	61678.0
23	46632.0
24	52447.0
25	58262.0
26	58262.0
27	64313.0
28	70364.0
29	76908.5
30	83453.0
31	92332.5
32	101212.0
33	101212.0
34	113001.0
35	124790.0
36	137888.5
37	150987.0
38	162613.5
39	174240.0
40	174240.0
41	181559.0
42	188878.0
43	202349.5
44	215821.0
45	227493.5
46	239166.0
47	239166.0
48	295150.0
49	351134.0
50	336761.0
51	322388.0
52	301583.0
53	280778.0
54	280778.0
55	253583.5
56	226389.0
57	196142.5
58	165896.0
59	149102.0
60	132308.0
61	132308.0
62	117706.0
63	103104.0
64	89799.5
65	76495.0
66	65251.5
67	54008.0
68	54008.0
69	46494.5
70	38981.0
71	33147.5
72	27314.0
73	22471.0
74	17628.0
75	17628.0
76	14316.0
77	11004.0
78	8597.5
79	6191.0
80	4769.5
81	3348.0
82	3348.0
83	2505.0
84	1662.0
85	1310.0
86	958.0
87	708.5
88	459.0
89	459.0
90	386.5
91	314.0
92	209.0
93	104.0
94	75.5
95	47.0
96	47.0
97	33.0
98	19.0
99	10.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3556139.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.82617172295789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.17931620311093	35.82658769507185
2	11.833323354807243	10.845518162213697
3	4.054605983430958	5.574212101969189
4	1.8343687862026252	3.362483959990213
5	0.9593672883513706	2.1982065050689186
6	0.5785481018280231	1.5907586798617386
7	0.38436966381934157	1.2329933153496517
8	0.28921489226101255	1.0602889054071938
9	0.2325710454110885	0.9592056598316742
>10	1.3843621697153923	12.340084347541941
>50	0.15216072689441246	4.83081266554726
>100	0.10334403373243387	9.192151631399865
>500	0.00922196836374556	2.938023728235115
>1k	0.004610984180761374	3.7721100749021295
>5k	3.6887873446090995E-4	1.1833291899584302
>10k+	2.459191563072733E-4	3.093233377651152
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35887	1.009156278761882	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34380	0.9667788576318304	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25375	0.7135547851194793	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14149	0.3978753361440596	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8063	0.22673466925786645	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7450	0.2094968728725171	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7113	0.20002030291841796	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7113	0.20002030291841796	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6705	0.18854718558526537	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5557	0.1562649828929634	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4139	0.11639027608313399	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3866	0.10871341080874511	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3811	0.10716678959961914	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3790	0.10657626150158922	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.812038562047209E-5	0.0	0.0	0.0	0.0
6	8.436115686141627E-5	0.0	0.0	0.0	0.0
7	8.436115686141627E-5	0.0	0.0	0.0	0.0
8	1.1248154248188836E-4	0.0	0.0	0.0	0.0
9	1.6872231372283254E-4	0.0	0.0	1.1248154248188836E-4	0.0
10	2.2496308496377672E-4	0.0	0.0	1.9684269934330464E-4	0.0
11	2.530834705842488E-4	0.0	0.0	3.655650130661372E-4	0.0
12	2.812038562047209E-4	0.0	0.0	7.030096405118022E-4	0.0
13	2.812038562047209E-4	0.0	0.0	7.873707973732186E-4	0.0
14	3.655650130661372E-4	0.0	0.0	8.998523398551069E-4	0.0
15	3.655650130661372E-4	0.0	0.0	0.0013216581241621882	2.812038562047209E-5
16	3.936853986866093E-4	0.0	0.0	0.00188406583657163	2.812038562047209E-5
17	3.936853986866093E-4	0.0	0.0	0.003065122032631458	2.812038562047209E-5
18	4.2180578430708133E-4	0.0	0.0	0.003430687045697595	2.812038562047209E-5
19	4.2180578430708133E-4	0.0	0.0	0.004583622856136951	2.812038562047209E-5
20	4.2180578430708133E-4	0.0	0.0	0.007845587588111713	2.812038562047209E-5
21	4.2180578430708133E-4	0.0	0.0	0.012907256999796689	2.812038562047209E-5
22	4.2180578430708133E-4	0.0	0.0	0.02030291841798085	2.812038562047209E-5
23	4.2180578430708133E-4	0.0	0.0	0.025983236313316213	2.812038562047209E-5
24	5.061669411684976E-4	0.0	0.0	0.03495363932624681	2.812038562047209E-5
25	5.061669411684976E-4	0.0	0.0	0.041112003777130195	2.812038562047209E-5
26	5.061669411684976E-4	0.0	0.0	0.05112286105801826	2.812038562047209E-5
27	5.061669411684976E-4	0.0	0.0	0.10083970283501292	2.812038562047209E-5
28	5.342873267889697E-4	0.0	0.0	0.20831581667645724	2.812038562047209E-5
29	5.624077124094418E-4	0.0	0.0	0.3417751668312178	2.812038562047209E-5
30	5.905280980299139E-4	0.0	0.0	0.531503408612543	2.812038562047209E-5
31	5.905280980299139E-4	0.0	0.0	0.8106544766669694	2.812038562047209E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11910	0.0	21.746431	1
GTATTAG	880	0.0	16.1875	1
CTAATAC	915	0.0	15.366119	3
TAATACT	935	0.0	15.037434	4
AATACTG	935	0.0	15.037434	5
TACGTGC	185	3.0559022E-10	15.0	8
GTATTGG	1755	0.0	14.757834	1
GTATCAA	17565	0.0	14.703103	2
TCTAATA	965	0.0	14.569947	2
TTACGCA	90	8.280022E-4	14.388889	4
CTCTAAT	810	0.0	14.388889	1
TTGGACC	2415	0.0	14.171843	4
ATTAGAG	795	0.0	13.962263	3
TTAACGG	545	0.0	13.917431	35
TGGACCC	2455	0.0	13.8655815	5
TATACCG	255	1.8189894E-12	13.784313	5
TTATGCG	420	0.0	13.654763	4
TAAACGT	190	7.1413524E-9	13.631579	4
ACCGTTA	560	0.0	13.544643	8
GGTAAGA	580	0.0	13.396551	2
>>END_MODULE