FastQCFastQC Report
Wed 25 May 2016
SRR2088593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3358911
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT304290.9059186146938696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT288270.8582245852896966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT210360.6262744085806382No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120260.3580327076245843No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG61950.18443477662849656No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA61250.18235076785303334No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA58460.17404450430511556No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG56920.16945968499909644No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA54130.16115342145117867No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG45320.13492468243427705No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34080.10146145581112449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100400.022.3510951
ACCGTTA5900.016.9322038
GTATTGG14650.016.2901041
GACCGTT6750.015.6222237
GTATCAA144700.015.4571522
GTCTAAC2900.015.3103451
ATTGGAC16850.015.1513373
GTATTAG6600.014.2954541
TTGGACC19550.014.1943734
CCGTTTA7100.014.07042227
TATACCG1458.923889E-714.0344835
GGACCGT8050.014.0186346
CGTTTAC7850.013.6687928
CGCCGTT7200.013.61805625
CTAATAC7100.013.5492953
CCGTTAT7600.013.3881589
TAGACTG3350.013.2537325
ACCGTGT4050.013.2469148
TGGACCC20800.013.1634625
CCGTGTT1551.8903993E-613.1290329
Produced by FastQC (version 0.11.5)