FastQCFastQC Report
Wed 25 May 2016
SRR2088592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013114
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT300310.9966765280039189No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT295900.9820405069307037No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT221900.736447409557023No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121740.4040338334361063No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG78980.26212084906180116No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA74550.24741845147578218No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA73680.24453107316882136No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA67100.2226932004564049No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG65630.21781452676533317No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG55840.18532322374792326No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA43320.14377152673280866No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA37120.12319480776366244No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG36860.12233191309721438No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC35230.11692222730371304No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34060.11303920130469675No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33600.1115125415102117No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31940.1060032909475048No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA30990.10285040658932919No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC30280.10049404038479791No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA30270.10046085212839607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96100.024.4677431
CCGTATA1400.021.1428572
CGTATAC1457.2759576E-1219.137933
GTATTGG15950.018.789971
GAACCGT1156.41503E-817.6956526
ATTGGAC18450.017.4471553
TTGGACC22550.016.8181824
GTATCAA145150.016.1739562
GTATTAG7650.015.4771241
TGGACCC23600.015.0508475
CGAACTA4850.014.87628824
GGACCCT23550.014.8471346
GTATACT3000.014.84
CTCTAAT5950.014.6134451
TCTATAC2550.014.5098043
ATTAGAG6250.014.5043
TTAACGG4600.014.47826135
ATACTGG6850.014.31386856
TATACTG3750.014.3066675
TATTGGA21350.014.0374712
Produced by FastQC (version 0.11.5)