##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088592_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3013114
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71559157735154	31.0	31.0	34.0	30.0	34.0
2	31.900584909830826	31.0	31.0	34.0	30.0	34.0
3	31.97537431374983	31.0	31.0	34.0	30.0	34.0
4	35.65917917476737	37.0	35.0	37.0	33.0	37.0
5	35.57035877168936	37.0	35.0	37.0	33.0	37.0
6	35.60451048317455	37.0	35.0	37.0	33.0	37.0
7	35.47666931951463	37.0	35.0	37.0	33.0	37.0
8	35.49752183289447	37.0	35.0	37.0	33.0	37.0
9	37.110594222455575	39.0	37.0	39.0	33.0	39.0
10	36.97841070732803	39.0	37.0	39.0	33.0	39.0
11	37.01646436211839	39.0	37.0	39.0	33.0	39.0
12	36.92384622686032	39.0	37.0	39.0	33.0	39.0
13	36.96380389192045	39.0	37.0	39.0	33.0	39.0
14	38.01562436734886	40.0	37.0	41.0	33.0	41.0
15	37.974108181768095	40.0	37.0	41.0	33.0	41.0
16	37.86575416661965	40.0	37.0	41.0	32.0	41.0
17	37.8934451202311	40.0	37.0	41.0	33.0	41.0
18	37.866173334298004	40.0	37.0	41.0	33.0	41.0
19	37.889363296576235	40.0	37.0	41.0	32.0	41.0
20	37.76614691644591	40.0	37.0	41.0	32.0	41.0
21	37.66376811498005	40.0	37.0	41.0	32.0	41.0
22	37.51677434043319	39.0	36.0	41.0	32.0	41.0
23	37.38352647792284	39.0	36.0	41.0	31.0	41.0
24	37.2443754202463	39.0	36.0	41.0	31.0	41.0
25	37.05686774546201	39.0	36.0	41.0	31.0	41.0
26	37.20874782699891	39.0	36.0	41.0	31.0	41.0
27	37.142371314195216	39.0	36.0	41.0	31.0	41.0
28	37.02358423876428	39.0	36.0	41.0	31.0	41.0
29	36.88291083576659	39.0	35.0	41.0	30.0	41.0
30	36.766138951264374	39.0	35.0	41.0	30.0	41.0
31	36.579777266973636	39.0	35.0	40.0	30.0	41.0
32	36.45826709510493	39.0	35.0	40.0	30.0	41.0
33	36.25661923179807	38.0	35.0	40.0	30.0	41.0
34	36.11657109555098	38.0	35.0	40.0	30.0	41.0
35	35.934093432906955	38.0	35.0	40.0	29.0	41.0
36	35.794705079197136	38.0	35.0	40.0	27.0	41.0
37	35.628896882096065	38.0	35.0	40.0	27.0	41.0
38	35.544816425797364	38.0	35.0	40.0	26.0	41.0
39	35.408848453792324	38.0	34.0	40.0	26.0	41.0
40	35.232153844826314	38.0	34.0	40.0	25.0	41.0
41	35.10552504817275	38.0	34.0	40.0	24.0	41.0
42	34.88937325305315	38.0	34.0	40.0	23.0	41.0
43	34.54019263791546	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	0.0
13	3.0
14	8.0
15	22.0
16	42.0
17	90.0
18	190.0
19	422.0
20	946.0
21	2098.0
22	3858.0
23	6530.0
24	10677.0
25	16341.0
26	24039.0
27	34009.0
28	46359.0
29	61382.0
30	76864.0
31	95043.0
32	117945.0
33	145100.0
34	179354.0
35	224482.0
36	293047.0
37	417626.0
38	582660.0
39	673973.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.82566607171186	20.982445403658804	13.5825262502514	23.609362274377936
2	19.064595630965176	22.60740881360612	35.167736766680584	23.16025878874812
3	19.991477255756003	24.473252588518058	32.09573218935626	23.439537966369674
4	14.07490722222923	16.063215663263986	35.91679571367031	33.94508140083647
5	13.22545379962391	37.807597057396436	34.9544690310423	14.01248011193735
6	32.88903108212965	36.477345364297534	13.760315739796106	16.873307813776712
7	26.46471391391099	32.4429477278324	21.651288334925265	19.441050023331343
8	26.672040951653344	34.57901028636819	19.13648139433158	19.612467367646893
9	24.476737355440253	14.525271861602315	21.806011986270683	39.19197879668675
10	16.750743582884684	26.830315746433758	33.63739307573494	22.781547594946623
11	33.22310407107066	22.007995714732335	23.53113755403878	21.23776266015823
12	20.96117173130522	26.604237343824362	30.954620369491497	21.479970555378923
13	31.26131304690098	20.56201657156019	26.437001719815445	21.739668661723385
14	20.646547060615696	22.58503992879128	27.43776040335679	29.330652607236235
15	23.208879584376827	28.79217314711624	25.373218537366988	22.625728731139944
16	22.254252577234052	27.88072406155227	26.557209584502946	23.307813776710738
17	22.0091573037064	26.244012008838695	26.996522534494215	24.75030815296069
18	23.17818044720512	25.202332205153873	29.215157474957802	22.40432987268321
19	23.181200578537688	24.51643051009686	30.007626661321147	22.294742250044305
20	24.112662182711972	24.110040310456228	29.98562284732672	21.791674659505084
21	23.0378604991381	25.002638466383946	30.277845444944994	21.681655589532955
22	21.762601746897065	25.376371421725164	29.971716967894345	22.889309863483426
23	22.55656440479849	25.55591988885917	30.979577938305685	20.907937768036653
24	22.752441494082202	25.544403563887723	28.560519117431337	23.142635824598738
25	22.692901762097286	25.716551049844117	29.410238046087866	22.18030914197073
26	22.527856563010893	26.216897203358386	29.507081378268463	21.74816485536226
27	21.560485265409802	26.474537637805938	29.160064969330733	22.804912127453527
28	21.898872727683056	25.99168169541544	29.411432823318336	22.698012753583168
29	22.20324222714441	26.023243727253597	28.90962638652238	22.86388765907961
30	22.200122531042634	26.032337309507703	30.2688514274601	21.498688731989564
31	22.628416979908494	25.582802376544667	30.275124007920045	21.513656635626795
32	22.355012123670065	25.481976453595852	29.282430070684352	22.880581352049738
33	21.979818885047166	25.6390896594022	29.91307331883228	22.46801813671836
34	21.418273586727885	26.173121893164346	30.252555993566787	22.15604852654098
35	21.208855688832216	27.861043425505972	29.188640058092723	21.741460827569085
36	22.912077007375096	25.646590205349018	29.322056848828154	22.119275938447732
37	21.99734228442734	26.084675189853424	29.262483928586835	22.6554985971324
38	22.01728842652485	24.84413135380872	30.43967138316041	22.69890883650602
39	21.365902518125765	24.854519278062494	31.08289298048464	22.696685223327094
40	20.55972658186846	25.587216414646107	30.67603814525438	23.17701885823105
41	21.77906312207238	24.51901919409621	30.704414104477955	22.997503579353452
42	20.499158013935084	26.399366237055748	30.133443341340556	22.968032407668613
43	20.848298471282533	26.269201895447697	29.326006251339976	23.55649338192979
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	170.0
1	341.5
2	513.0
3	1461.0
4	2409.0
5	2409.0
6	3651.5
7	4894.0
8	5369.0
9	5844.0
10	8733.0
11	11622.0
12	11622.0
13	21509.0
14	31396.0
15	46948.0
16	62500.0
17	58305.5
18	54111.0
19	54111.0
20	60151.0
21	66191.0
22	52799.5
23	39408.0
24	44144.5
25	48881.0
26	48881.0
27	53312.0
28	57743.0
29	63709.5
30	69676.0
31	77370.0
32	85064.0
33	85064.0
34	94684.0
35	104304.0
36	114923.5
37	125543.0
38	134432.0
39	143321.0
40	143321.0
41	149933.0
42	156545.0
43	167896.5
44	179248.0
45	190865.5
46	202483.0
47	202483.0
48	257063.0
49	311643.0
50	296457.0
51	281271.0
52	262113.5
53	242956.0
54	242956.0
55	217363.5
56	191771.0
57	165082.0
58	138393.0
59	123721.0
60	109049.0
61	109049.0
62	96778.0
63	84507.0
64	74106.0
65	63705.0
66	54791.5
67	45878.0
68	45878.0
69	39562.0
70	33246.0
71	28113.0
72	22980.0
73	18990.0
74	15000.0
75	15000.0
76	12057.0
77	9114.0
78	7184.5
79	5255.0
80	4144.0
81	3033.0
82	3033.0
83	2320.5
84	1608.0
85	1237.5
86	867.0
87	663.5
88	460.0
89	460.0
90	396.0
91	332.0
92	215.5
93	99.0
94	76.0
95	53.0
96	53.0
97	36.5
98	20.0
99	14.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3013114.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.218363964398314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.78450562995832	36.412909559606014
2	11.490693280458446	10.62162088478989
3	3.8459513189960304	5.332607335521489
4	1.6679319329733993	3.083563405840279
5	0.9338966321776929	2.1581587225557213
6	0.60222085178504	1.670019750885057
7	0.4143704777349523	1.3406067889238942
8	0.28947008233794014	1.0703066897839413
9	0.23152034416931466	0.9630442378782134
>10	1.4781588553502354	13.177803886555568
>50	0.14830792833796935	4.7337872866222614
>100	0.09994482804700493	8.754832592658165
>500	0.008133401420572384	2.546557736315825
>1k	0.004174666215857912	3.624461900542223
>5k	4.318620223301289E-4	1.3831584266534305
>10k+	2.8790801488675255E-4	3.1265607948680234
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30031	0.9966765280039189	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29590	0.9820405069307037	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22190	0.736447409557023	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12174	0.4040338334361063	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7898	0.26212084906180116	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7455	0.24741845147578218	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7368	0.24453107316882136	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6710	0.2226932004564049	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6563	0.21781452676533317	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5584	0.18532322374792326	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4332	0.14377152673280866	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3712	0.12319480776366244	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3686	0.12233191309721438	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3523	0.11692222730371304	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3406	0.11303920130469675	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3360	0.1115125415102117	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3194	0.1060032909475048	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3099	0.10285040658932919	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3028	0.10049404038479791	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3027	0.10046085212839607	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.318825640184872E-5
2	0.0	0.0	0.0	0.0	3.318825640184872E-5
3	0.0	0.0	0.0	0.0	3.318825640184872E-5
4	0.0	0.0	0.0	0.0	3.318825640184872E-5
5	0.0	0.0	0.0	0.0	3.318825640184872E-5
6	3.318825640184872E-5	0.0	0.0	0.0	3.318825640184872E-5
7	3.318825640184872E-5	0.0	0.0	0.0	3.318825640184872E-5
8	6.637651280369744E-5	0.0	0.0	0.0	3.318825640184872E-5
9	6.637651280369744E-5	0.0	0.0	9.956476920554615E-5	3.318825640184872E-5
10	6.637651280369744E-5	0.0	0.0	2.3231779481294103E-4	3.318825640184872E-5
11	9.956476920554615E-5	0.0	0.0	4.978238460277308E-4	3.318825640184872E-5
12	1.327530256073949E-4	0.0	0.0	9.624594356536128E-4	3.318825640184872E-5
13	1.327530256073949E-4	0.0	0.0	0.0010952124612610076	3.318825640184872E-5
14	1.327530256073949E-4	0.0	0.0	0.0015598480508868897	3.318825640184872E-5
15	1.327530256073949E-4	0.0	0.0	0.001891730614905377	3.318825640184872E-5
16	1.327530256073949E-4	0.0	0.0	0.0031196961017737794	3.318825640184872E-5
17	1.6594128200924358E-4	0.0	0.0	0.0043808498450440304	3.318825640184872E-5
18	1.6594128200924358E-4	0.0	0.0	0.005243744511492098	3.318825640184872E-5
19	2.655060512147898E-4	0.0	0.0	0.006504898254762349	3.318825640184872E-5
20	2.655060512147898E-4	0.0	0.0	0.010288359484573103	3.318825640184872E-5
21	2.9869430761663844E-4	0.0	0.0	0.01742383461097058	3.318825640184872E-5
22	2.9869430761663844E-4	3.318825640184872E-5	0.0	0.0284755239927862	3.318825640184872E-5
23	2.9869430761663844E-4	3.318825640184872E-5	0.0	0.0362415759908188	3.318825640184872E-5
24	3.3188256401848717E-4	3.318825640184872E-5	0.0	0.048222536551886186	3.318825640184872E-5
25	3.3188256401848717E-4	3.318825640184872E-5	0.0	0.05691785972917055	6.637651280369744E-5
26	3.650708204203359E-4	3.318825640184872E-5	0.0	0.0673721604957529	6.637651280369744E-5
27	3.650708204203359E-4	3.318825640184872E-5	0.0	0.11433354330436883	6.637651280369744E-5
28	3.982590768221846E-4	3.318825640184872E-5	0.0	0.22730636809626187	6.637651280369744E-5
29	3.982590768221846E-4	3.318825640184872E-5	0.0	0.3721399190339297	6.637651280369744E-5
30	3.982590768221846E-4	3.318825640184872E-5	0.0	0.5723978581626848	1.6594128200924358E-4
31	4.3144733322403333E-4	3.318825640184872E-5	0.0	0.8611025006023668	1.6594128200924358E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9610	0.0	24.467743	1
CCGTATA	140	0.0	21.142857	2
CGTATAC	145	7.2759576E-12	19.13793	3
GTATTGG	1595	0.0	18.78997	1
GAACCGT	115	6.41503E-8	17.695652	6
ATTGGAC	1845	0.0	17.447155	3
TTGGACC	2255	0.0	16.818182	4
GTATCAA	14515	0.0	16.173956	2
GTATTAG	765	0.0	15.477124	1
TGGACCC	2360	0.0	15.050847	5
CGAACTA	485	0.0	14.876288	24
GGACCCT	2355	0.0	14.847134	6
GTATACT	300	0.0	14.8	4
CTCTAAT	595	0.0	14.613445	1
TCTATAC	255	0.0	14.509804	3
ATTAGAG	625	0.0	14.504	3
TTAACGG	460	0.0	14.478261	35
ATACTGG	685	0.0	14.3138685	6
TATACTG	375	0.0	14.306667	5
TATTGGA	2135	0.0	14.037471	2
>>END_MODULE