FastQCFastQC Report
Wed 25 May 2016
SRR2088591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938038
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT109171.1638121270140442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108721.1590148799942006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64400.6865393512842763No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42280.4507280088866336No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA18690.19924565955750195No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG18450.19668712781358536No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG18030.1922096972617314No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16660.17760474522354106No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA16440.1752594244582842No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12770.13613520987422684No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12760.13602860438489697No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG12170.12973888051443544No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9430.10052897643805475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32950.025.8270111
TACACAG1156.4012056E-817.695655
GTATCAA48700.017.3983572
TCGGGTA650.001579640917.07692333
GCGATAC650.001579640917.07692313
TGACTAA650.001579640917.07692312
CGCGATA700.002592017615.85714212
TATACTT1557.201379E-915.51612955
TAGTTCG855.362845E-415.2352949
CGGGTAA750.004103657314.834
ACGGGTA1508.094321E-814.85
TCGCGAT750.004103657314.811
GTCGGGT750.004103657314.832
CTAAAGA1304.4415738E-614.2307692
GACTGAG1458.904153E-714.0344837
TAAACGT800.006297481213.8750014
CTCGCGA800.006297481213.87500110
CCGAAAT800.006297481213.87500124
TATACAG1601.781791E-713.8750015
ACTGTTC1203.2990873E-513.8749998
Produced by FastQC (version 0.11.5)