##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088591_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	938038
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15886563230914	31.0	31.0	33.0	30.0	34.0
2	31.3638445350828	31.0	31.0	34.0	30.0	34.0
3	31.38367848637262	31.0	31.0	34.0	28.0	34.0
4	35.15557258874374	37.0	35.0	37.0	32.0	37.0
5	35.00783017319128	35.0	35.0	37.0	32.0	37.0
6	35.111004031819604	36.0	35.0	37.0	32.0	37.0
7	34.994394683371034	36.0	35.0	37.0	32.0	37.0
8	35.02796688407079	37.0	35.0	37.0	32.0	37.0
9	36.559355804349075	38.0	35.0	39.0	32.0	39.0
10	36.32446766548903	38.0	35.0	39.0	32.0	39.0
11	36.43302616738341	38.0	35.0	39.0	32.0	39.0
12	36.27771689419831	38.0	35.0	39.0	32.0	39.0
13	36.362245452742854	38.0	35.0	39.0	32.0	39.0
14	37.234038493110084	39.0	36.0	40.0	32.0	41.0
15	37.2155520352054	39.0	36.0	40.0	32.0	41.0
16	37.04671985569881	39.0	36.0	40.0	31.0	41.0
17	37.10568548395694	39.0	36.0	40.0	31.0	41.0
18	37.070124024826285	39.0	36.0	40.0	31.0	41.0
19	37.10132105522378	39.0	36.0	40.0	31.0	41.0
20	36.97351493223089	39.0	36.0	40.0	31.0	41.0
21	36.86434877904733	39.0	36.0	40.0	31.0	41.0
22	36.691054093757394	39.0	35.0	40.0	30.0	41.0
23	36.54421142853488	38.0	35.0	40.0	30.0	41.0
24	36.391850863184644	38.0	35.0	40.0	30.0	41.0
25	36.189988038864094	38.0	35.0	40.0	29.0	41.0
26	36.309315827290575	38.0	35.0	40.0	30.0	41.0
27	36.20467720923886	38.0	35.0	40.0	30.0	41.0
28	36.05421635370848	38.0	35.0	40.0	29.0	41.0
29	35.88929552960541	38.0	34.0	40.0	28.0	41.0
30	35.784713412463034	38.0	34.0	40.0	28.0	41.0
31	35.58642507019972	38.0	34.0	40.0	27.0	41.0
32	35.4588779985459	38.0	34.0	40.0	27.0	41.0
33	35.23410778667815	38.0	34.0	40.0	26.0	41.0
34	35.07313562989985	38.0	33.0	40.0	26.0	41.0
35	34.885858568629416	38.0	33.0	40.0	25.0	41.0
36	34.733414850997505	38.0	33.0	40.0	25.0	41.0
37	34.58122272232042	38.0	33.0	40.0	24.0	41.0
38	34.4738955138278	38.0	33.0	40.0	24.0	41.0
39	34.30227560077523	37.0	33.0	40.0	23.0	41.0
40	34.10398512640213	37.0	33.0	40.0	22.0	41.0
41	33.96706423407154	37.0	33.0	40.0	21.0	41.0
42	33.74076103526723	37.0	32.0	40.0	20.0	41.0
43	33.36265268571209	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	5.0
15	7.0
16	29.0
17	49.0
18	126.0
19	303.0
20	581.0
21	1181.0
22	2050.0
23	3459.0
24	5391.0
25	7829.0
26	11041.0
27	14963.0
28	19870.0
29	25368.0
30	31065.0
31	38125.0
32	45862.0
33	55707.0
34	66894.0
35	82227.0
36	107091.0
37	142845.0
38	176589.0
39	99379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.32495272046548	23.602775154098236	15.291171573006638	21.781100552429645
2	21.092322485869442	24.62501519128223	32.66520119654001	21.617461126308317
3	21.98663593585761	25.171688140565735	31.822378197898164	21.01929772567849
4	16.445389205980995	18.451597909679567	34.17633400779073	30.926678876548714
5	13.130704726247764	39.321967766764246	34.139874930439916	13.40745257654807
6	32.19645685995663	37.54090985653033	13.659574558813182	16.603058724699853
7	25.7164421910413	33.3678379767131	21.522049213358095	19.39367061888751
8	27.051249522940434	35.43108061720314	18.787405201068612	18.73026465878781
9	24.9234039559165	14.461034627595042	21.432713813299674	39.18284760318878
10	17.097388378722396	27.920510682936083	33.0936486581567	21.88845228018481
11	33.74106379485692	22.72221381223362	22.703771062579552	20.8329513303299
12	20.36729855293709	27.79077180242165	30.860796684142862	20.981132960498403
13	31.79060976207787	21.01268818534004	26.167490016395924	21.029212036186166
14	20.45428863223025	23.14479797193717	27.923175820169334	28.477737575663248
15	23.753515316010652	29.56756549308237	24.91348964540882	21.765429545498158
16	21.9101998000081	28.167195785245376	27.107217404838607	22.815387009907916
17	22.006997584319613	27.401128738921027	27.311473522394614	23.28040015436475
18	22.72924977452939	25.73253961993011	29.127924455086042	22.410286150454457
19	23.164093565505876	25.284370142787395	30.33288630098141	21.21864999072532
20	23.681343399734338	24.825113694754368	30.257303009046545	21.23623989646475
21	23.01132789929619	25.310488487673204	30.76005449672615	20.918129116304456
22	21.721294872915596	25.886371341033094	30.300904654182453	22.09142913186886
23	22.16541334146378	26.128045985343878	30.946827314032056	20.75971335916029
24	22.190892053413613	26.045533336602567	29.39550423330398	22.368070376679835
25	22.36753734923319	26.322707608860195	29.920642873742853	21.389112168163763
26	22.155179214488115	26.628452152258227	29.98961662533927	21.226752007914392
27	21.729396890104667	26.60777068732823	29.71158950916701	21.951242913400097
28	21.623750850178777	26.647108112890947	29.853588020954376	21.8755530159759
29	21.789735597065363	26.256079178029033	29.570017419336956	22.384167805568644
30	21.804980182039532	26.696679665429336	30.546523701598442	20.951816450932693
31	22.118080504201323	26.221858815954153	30.39823546594061	21.261825213903915
32	22.00305318121441	26.23028064961121	29.723422718482624	22.04324345069176
33	21.749118905630688	26.44082649103768	29.934075165398415	21.875979437933218
34	21.245407968547116	26.492530153362658	30.56805801044307	21.69400386764715
35	21.022495890358385	27.88191949579868	29.758282713493482	21.337301900349452
36	22.455700088908976	26.154590752187012	29.856359763676956	21.53334939522706
37	21.706370104409416	26.555107575599283	29.739200330903437	21.99932198908786
38	21.721934505851575	25.48574791213149	30.777964218933562	22.014353363083373
39	21.19903458068863	25.490118737194017	31.20342672684902	22.107419955268337
40	20.645219063620026	26.04414746524128	30.87582805813837	22.434805413000326
41	21.484417475624653	25.170195663715116	30.807707150456594	22.537679710203637
42	20.620913012052817	26.641458021956467	30.276491997125916	22.4611369688648
43	20.70747666938866	26.532933633818672	29.72630106669453	23.03328863009814
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	114.0
1	148.5
2	183.0
3	349.0
4	515.0
5	515.0
6	958.0
7	1401.0
8	1606.5
9	1812.0
10	2741.0
11	3670.0
12	3670.0
13	7241.0
14	10812.0
15	16745.0
16	22678.0
17	20321.5
18	17965.0
19	17965.0
20	20765.0
21	23565.0
22	18892.5
23	14220.0
24	15527.0
25	16834.0
26	16834.0
27	18462.5
28	20091.0
29	21809.5
30	23528.0
31	25991.0
32	28454.0
33	28454.0
34	31211.0
35	33968.0
36	37877.5
37	41787.0
38	44176.0
39	46565.0
40	46565.0
41	48363.5
42	50162.0
43	53535.0
44	56908.0
45	60397.0
46	63886.0
47	63886.0
48	77294.5
49	90703.0
50	86761.0
51	82819.0
52	76873.5
53	70928.0
54	70928.0
55	63452.5
56	55977.0
57	49688.5
58	43400.0
59	38507.5
60	33615.0
61	33615.0
62	29677.5
63	25740.0
64	22169.5
65	18599.0
66	15632.5
67	12666.0
68	12666.0
69	10837.0
70	9008.0
71	7585.0
72	6162.0
73	5078.5
74	3995.0
75	3995.0
76	3173.5
77	2352.0
78	1840.5
79	1329.0
80	1050.5
81	772.0
82	772.0
83	579.0
84	386.0
85	298.0
86	210.0
87	170.5
88	131.0
89	131.0
90	104.5
91	78.0
92	56.5
93	35.0
94	22.5
95	10.0
96	10.0
97	6.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	938038.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.749259781704524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.78560697412057	52.971977305761754
2	8.224065798638689	10.156599509239435
3	2.2954240559040953	4.252222090115875
4	1.0541539390666572	2.6037290173333663
5	0.5629127570169167	1.7379723033736552
6	0.3899671882148864	1.4448111126853131
7	0.26839946105037543	1.1601427631968384
8	0.20930774629078697	1.0339678720026324
9	0.14931966505873476	0.8298340909406031
>10	0.9527981034772388	10.98949418622249
>50	0.07064723661381339	3.0164121430426882
>100	0.03254930575490167	3.8430698864207864
>500	0.0027701535956423957	1.1430745965388331
>1k	0.0015582113975488475	1.7987495404756704
>5k	1.7313459972764973E-4	0.6884961094004943
>10k+	3.4626919945529946E-4	2.3294474732495916
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10917	1.1638121270140442	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10872	1.1590148799942006	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6440	0.6865393512842763	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4228	0.4507280088866336	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1869	0.19924565955750195	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1845	0.19668712781358536	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1803	0.1922096972617314	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1666	0.17760474522354106	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1644	0.1752594244582842	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1277	0.13613520987422684	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1276	0.13602860438489697	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1217	0.12973888051443544	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	943	0.10052897643805475	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0660548932985657E-4	0.0
5	0.0	0.0	0.0	1.0660548932985657E-4	0.0
6	0.0	0.0	0.0	1.0660548932985657E-4	0.0
7	0.0	0.0	0.0	1.0660548932985657E-4	0.0
8	0.0	0.0	0.0	1.0660548932985657E-4	0.0
9	0.0	0.0	0.0	2.1321097865971314E-4	0.0
10	0.0	0.0	0.0	2.1321097865971314E-4	0.0
11	0.0	0.0	0.0	6.396329359791395E-4	0.0
12	0.0	0.0	0.0	0.001279265871958279	0.0
13	0.0	0.0	0.0	0.0013858713612881354	0.0
14	0.0	0.0	0.0	0.001492476850617992	0.0
15	0.0	0.0	0.0	0.0019188988079374184	0.0
16	0.0	0.0	0.0	0.0026651372332464145	0.0
17	0.0	0.0	0.0	0.003837797615874837	0.0
18	0.0	0.0	0.0	0.0042642195731942625	0.0
19	0.0	0.0	0.0	0.005436879955822685	0.0
20	0.0	0.0	0.0	0.00874165012504824	0.0
21	0.0	0.0	0.0	0.014071924591541067	0.0
22	0.0	0.0	0.0	0.023239996673908733	0.0
23	0.0	0.0	1.0660548932985657E-4	0.02952972054437027	0.0
24	0.0	0.0	1.0660548932985657E-4	0.040190269477355926	0.0
25	0.0	0.0	1.0660548932985657E-4	0.04477430551853976	0.0
26	0.0	0.0	1.0660548932985657E-4	0.05276971721827901	0.0
27	0.0	0.0	1.0660548932985657E-4	0.09519870197156192	0.0
28	0.0	0.0	1.0660548932985657E-4	0.1939153850910091	0.0
29	0.0	0.0	1.0660548932985657E-4	0.33132986083719423	0.0
30	0.0	0.0	1.0660548932985657E-4	0.5050968084448605	0.0
31	0.0	0.0	1.0660548932985657E-4	0.7816314477665084	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3295	0.0	25.827011	1
TACACAG	115	6.4012056E-8	17.69565	5
GTATCAA	4870	0.0	17.398357	2
TCGGGTA	65	0.0015796409	17.076923	33
GCGATAC	65	0.0015796409	17.076923	13
TGACTAA	65	0.0015796409	17.076923	12
CGCGATA	70	0.0025920176	15.857142	12
TATACTT	155	7.201379E-9	15.5161295	5
TAGTTCG	85	5.362845E-4	15.235294	9
CGGGTAA	75	0.0041036573	14.8	34
ACGGGTA	150	8.094321E-8	14.8	5
TCGCGAT	75	0.0041036573	14.8	11
GTCGGGT	75	0.0041036573	14.8	32
CTAAAGA	130	4.4415738E-6	14.230769	2
GACTGAG	145	8.904153E-7	14.034483	7
TAAACGT	80	0.0062974812	13.875001	4
CTCGCGA	80	0.0062974812	13.875001	10
CCGAAAT	80	0.0062974812	13.875001	24
TATACAG	160	1.781791E-7	13.875001	5
ACTGTTC	120	3.2990873E-5	13.874999	8
>>END_MODULE