FastQCFastQC Report
Wed 25 May 2016
SRR2088590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3453883
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT409351.1851878016713364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT396941.1492572273004036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT299840.8681243690072883No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164310.47572543713843235No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG104410.3022974431965414No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA95040.2751685566650636No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA93860.27175211204316996No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG82580.23909321769150838No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA80720.23370797447394717No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG68270.19766158842091638No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA56500.1635840009635532No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG49420.14308533323219114No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA48690.14097177003390096No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45790.1325754230817894No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC44150.12782714411576768No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42240.12229713629558384No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42100.12189179540824052No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC41120.1190544091968373No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA40820.11818582158110162No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA39690.11491414156183057No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC39430.11416136562819297No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC36780.10648884168919445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128600.022.2258951
ACGTTTA2950.018.81355926
ACCGTTA4850.016.7835048
TCACGTT3200.016.187524
GTATTGG18550.015.8571421
CGTGATT700.002593905215.85714212
GTATTAG6450.015.7751931
CACGTTT3450.015.55072425
TTAACGG4050.015.53086435
TAATACT7700.015.3766234
ATTAGAG6150.015.3414633
ATTGGAC19650.015.1577623
GTCGTAC750.00410662114.81
GTATCAA193750.014.7236132
CTAATAC7050.014.6950363
TTGGACC26850.014.6759784
CGTTATT2650.014.660377510
CCGTTTA5150.014.36893227
TATACTG4400.013.8755
CGTTTAC5600.013.87528
Produced by FastQC (version 0.11.5)