##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088590_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3453883
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71623010970551	31.0	31.0	34.0	30.0	34.0
2	31.89060457461935	31.0	31.0	34.0	30.0	34.0
3	31.965780832761272	31.0	31.0	34.0	30.0	34.0
4	35.646360921895734	37.0	35.0	37.0	33.0	37.0
5	35.56383612299548	37.0	35.0	37.0	33.0	37.0
6	35.60375612028549	37.0	35.0	37.0	33.0	37.0
7	35.473108672181425	37.0	35.0	37.0	33.0	37.0
8	35.499800369612984	37.0	35.0	37.0	33.0	37.0
9	37.104172318518025	39.0	37.0	39.0	33.0	39.0
10	36.98514512506648	39.0	37.0	39.0	33.0	39.0
11	37.022696194399174	39.0	37.0	39.0	33.0	39.0
12	36.934036271639776	39.0	37.0	39.0	33.0	39.0
13	36.97971182000085	39.0	37.0	39.0	33.0	39.0
14	38.01475006536122	40.0	37.0	41.0	33.0	41.0
15	37.97836956260534	40.0	37.0	41.0	33.0	41.0
16	37.87103066316954	40.0	37.0	41.0	32.0	41.0
17	37.916947968417	40.0	37.0	41.0	33.0	41.0
18	37.900516896490124	40.0	37.0	41.0	33.0	41.0
19	37.923555314409896	40.0	37.0	41.0	32.0	41.0
20	37.80810814958121	40.0	37.0	41.0	32.0	41.0
21	37.71200240425052	40.0	37.0	41.0	32.0	41.0
22	37.56840170903299	39.0	36.0	41.0	32.0	41.0
23	37.43514733996491	39.0	36.0	41.0	32.0	41.0
24	37.297392818459684	39.0	36.0	41.0	31.0	41.0
25	37.11039111631749	39.0	36.0	41.0	31.0	41.0
26	37.24597098396211	39.0	36.0	41.0	31.0	41.0
27	37.175639418011556	39.0	36.0	41.0	31.0	41.0
28	37.04930074353995	39.0	36.0	41.0	31.0	41.0
29	36.906973397767096	39.0	35.0	41.0	31.0	41.0
30	36.7732439112732	39.0	35.0	41.0	30.0	41.0
31	36.569862383873456	39.0	35.0	40.0	30.0	41.0
32	36.44321593985668	39.0	35.0	40.0	30.0	41.0
33	36.234448300651756	38.0	35.0	40.0	30.0	41.0
34	36.08597453938075	38.0	35.0	40.0	30.0	41.0
35	35.892629252351625	38.0	35.0	40.0	29.0	41.0
36	35.7412564351485	38.0	35.0	40.0	27.0	41.0
37	35.56112236575472	38.0	35.0	40.0	27.0	41.0
38	35.461592937572	38.0	35.0	40.0	26.0	41.0
39	35.31151981697122	38.0	34.0	40.0	25.0	41.0
40	35.11981992441551	38.0	34.0	40.0	24.0	41.0
41	34.974406776373144	38.0	34.0	40.0	24.0	41.0
42	34.734630269757254	38.0	34.0	40.0	23.0	41.0
43	34.37695428594426	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	7.0
14	6.0
15	14.0
16	52.0
17	100.0
18	252.0
19	533.0
20	1080.0
21	2300.0
22	4355.0
23	7490.0
24	12146.0
25	19179.0
26	27913.0
27	39414.0
28	54342.0
29	70930.0
30	89026.0
31	109966.0
32	134203.0
33	167107.0
34	206267.0
35	256055.0
36	334122.0
37	483431.0
38	657845.0
39	775742.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.777356094575296	21.15384336991149	13.989616903641497	23.07918363187172
2	18.787752798806444	22.968120228739654	35.50731162578466	22.736815346669243
3	20.090170975681573	24.691948163849208	32.3933671175312	22.82451374293802
4	14.007596667287226	16.10274580812378	35.92275708239104	33.966900442197954
5	13.048038975263493	38.0358570339528	34.824775477339564	14.091328513444143
6	32.566708252711514	36.37841814560597	13.752087143658311	17.3027864580242
7	26.070773097988553	32.730668641641884	21.692801985475477	19.505756274894082
8	26.688280986935574	34.569352812472225	19.196423272010083	19.54594292858212
9	24.238255899229937	14.694504706731525	22.32710256832672	38.740136825711815
10	16.784210698509476	26.932759447844646	33.59230755645168	22.690722297194203
11	32.6221820484365	22.203965797335925	23.910190356766574	21.263661797461005
12	20.909799202810287	26.81037545278749	31.255922681804797	21.02390266259743
13	31.437457493493554	20.539954596030032	26.807074819847692	21.215513090628722
14	20.456801808283604	22.927615092925848	27.940986999270095	28.674596099520457
15	22.910851351942146	28.918090161131687	25.886140323803673	22.28491816312249
16	21.705628129267843	28.02993037112143	27.140380840925992	23.124060658684733
17	21.590858752308634	26.337284731416784	27.42388783870212	24.64796867757246
18	22.97176829672574	25.0278309948542	29.844786288360087	22.155614420059973
19	22.756850767672212	24.485716510953033	30.89435861029456	21.8630741110802
20	23.798692659826635	23.986452349428166	30.818096617632968	21.396758373112235
21	22.60499269952109	24.996503934846665	31.290463515990552	21.108039849641695
22	21.311955268895908	25.32034235091345	30.835497322868203	22.53220505732244
23	22.128109145561677	25.543772038601194	31.82273400691338	20.505384808923754
24	22.286973820479734	25.594265931995956	29.40936331659179	22.709396930932517
25	22.257673464908915	25.806201310235465	30.24089119405608	21.695234030799536
26	22.101443505758592	26.327730267643695	30.33574096169442	21.2350852649033
27	20.992112355861504	26.469078425644412	30.13127543695024	22.407533781543844
28	21.39276287007985	26.077316458027095	30.249606023133964	22.28031464875909
29	21.64575348962313	26.03993823762994	29.710734266331546	22.60357400641539
30	21.649140981324496	26.240929411911175	31.232036522372063	20.877893084392262
31	22.294733203180307	25.696324976844902	31.103861943210003	20.90507987676479
32	21.90094453112627	25.617225597971903	30.00692264329741	22.474907227604408
33	21.481764147772232	25.793317260602056	30.60619019231399	22.11872839931173
34	20.9393311817453	26.305146989634565	30.99853121834179	21.756990610278347
35	20.56343541457542	28.21007544262501	29.950696071638788	21.275793071160777
36	22.514833305007727	25.925921636604365	29.941112654945172	21.618132403442733
37	21.59728630066508	26.456078564328898	29.91499712063205	22.031638014373968
38	21.509008845985807	25.14850676760041	31.09514711413212	22.247337272281662
39	20.905572076413705	24.934168296957367	31.874299158367553	22.285960468261372
40	19.94039751780822	25.880899845188733	31.30230526048508	22.876397376517964
41	21.32799518686649	24.82177305948117	31.28878424660013	22.561447507052208
42	19.949228158568197	26.783738765904925	30.699823937290287	22.56720913823659
43	20.248167062983892	26.740019855912895	29.881411732823608	23.1304013482796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	180.0
1	426.5
2	673.0
3	1996.0
4	3319.0
5	3319.0
6	5044.5
7	6770.0
8	7398.0
9	8026.0
10	11836.0
11	15646.0
12	15646.0
13	28858.0
14	42070.0
15	63685.5
16	85301.0
17	78989.0
18	72677.0
19	72677.0
20	78994.5
21	85312.0
22	67359.5
23	49407.0
24	54512.0
25	59617.0
26	59617.0
27	63955.0
28	68293.0
29	74075.0
30	79857.0
31	88057.0
32	96257.0
33	96257.0
34	106905.5
35	117554.0
36	129375.5
37	141197.0
38	149920.5
39	158644.0
40	158644.0
41	165749.5
42	172855.0
43	185993.5
44	199132.0
45	216755.5
46	234379.0
47	234379.0
48	304087.0
49	373795.0
50	352780.0
51	331765.0
52	306181.5
53	280598.0
54	280598.0
55	248579.0
56	216560.0
57	183783.0
58	151006.0
59	133174.0
60	115342.0
61	115342.0
62	101894.5
63	88447.0
64	76381.0
65	64315.0
66	55023.5
67	45732.0
68	45732.0
69	39082.5
70	32433.0
71	27549.0
72	22665.0
73	18628.5
74	14592.0
75	14592.0
76	11595.5
77	8599.0
78	6700.0
79	4801.0
80	3799.0
81	2797.0
82	2797.0
83	2117.0
84	1437.0
85	1110.0
86	783.0
87	656.0
88	529.0
89	529.0
90	437.0
91	345.0
92	223.0
93	101.0
94	76.0
95	51.0
96	51.0
97	32.5
98	14.0
99	12.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3453883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.10562207277114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.99607569842772	33.959598158413314
2	12.40874570857765	10.945908972392932
3	4.275895050063473	5.6577303330279705
4	1.9010366444624565	3.3538561514860037
5	1.0464687926707206	2.307757854024195
6	0.6213858876051387	1.6443966672039414
7	0.446151302306791	1.3774446508772593
8	0.3160853367761261	1.1152912325273925
9	0.22872349712835355	0.9079192911155144
>10	1.4822468780268532	12.563625624026239
>50	0.15478968151714662	4.764559281979154
>100	0.10916676605968731	9.276573473145564
>500	0.007634506168581952	2.3128919109331263
>1k	0.004870288417454005	4.4369857971583535
>5k	3.9488825006383827E-4	1.3845478735447492
>10k+	3.290735417198652E-4	3.9909127281443237
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40935	1.1851878016713364	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39694	1.1492572273004036	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29984	0.8681243690072883	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16431	0.47572543713843235	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10441	0.3022974431965414	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9504	0.2751685566650636	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9386	0.27175211204316996	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8258	0.23909321769150838	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8072	0.23370797447394717	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6827	0.19766158842091638	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5650	0.1635840009635532	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4942	0.14308533323219114	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4869	0.14097177003390096	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4579	0.1325754230817894	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4415	0.12782714411576768	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4224	0.12229713629558384	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4210	0.12189179540824052	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4112	0.1190544091968373	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4082	0.11818582158110162	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3969	0.11491414156183057	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3943	0.11416136562819297	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3678	0.10648884168919445	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.895292052452269E-5	0.0
3	2.895292052452269E-5	0.0	0.0	2.895292052452269E-5	0.0
4	2.895292052452269E-5	0.0	0.0	5.790584104904538E-5	0.0
5	2.895292052452269E-5	0.0	0.0	8.685876157356807E-5	0.0
6	2.895292052452269E-5	0.0	0.0	8.685876157356807E-5	0.0
7	2.895292052452269E-5	0.0	0.0	1.4476460262261346E-4	0.0
8	2.895292052452269E-5	0.0	0.0	1.7371752314713614E-4	0.0
9	2.895292052452269E-5	0.0	0.0	2.895292052452269E-4	0.0
10	8.685876157356807E-5	0.0	0.0	4.921996489168857E-4	0.0
11	2.3162336419618151E-4	0.0	0.0	8.685876157356806E-4	0.0
12	2.3162336419618151E-4	0.0	0.0	0.0018240339930449294	0.0
13	2.3162336419618151E-4	0.0	0.0	0.002026704436716588	0.0
14	2.3162336419618151E-4	0.0	0.0	0.0023162336419618153	0.0
15	2.3162336419618151E-4	0.0	0.0	0.0032137741782220187	0.0
16	2.3162336419618151E-4	0.0	0.0	0.0039665501118596085	0.0
17	2.3162336419618151E-4	0.0	0.0	0.005298384455987652	0.0
18	2.3162336419618151E-4	0.0	0.0	0.006195924992247855	0.0
19	2.895292052452269E-4	0.0	0.0	0.007933100223719217	0.0
20	3.1848212576974957E-4	0.0	0.0	0.012536614587118324	0.0
21	3.1848212576974957E-4	0.0	0.0	0.020614479413460157	0.0
22	3.7638796681879494E-4	0.0	0.0	0.033730152411068935	0.0
23	3.7638796681879494E-4	0.0	0.0	0.04316880450206333	0.0
24	3.7638796681879494E-4	0.0	0.0	0.057558406002751104	0.0
25	3.7638796681879494E-4	0.0	0.0	0.06714182269636812	0.0
26	4.0534088734331766E-4	0.0	0.0	0.08121294207128614	0.0
27	4.0534088734331766E-4	0.0	0.0	0.1369183611604678	0.0
28	4.342938078678403E-4	0.0	0.0	0.2626029891574208	0.0
29	4.342938078678403E-4	0.0	0.0	0.42859008252450936	0.0
30	4.342938078678403E-4	0.0	0.0	0.6575787309529593	0.0
31	4.6324672839236303E-4	0.0	0.0	0.9872945898862238	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12860	0.0	22.225895	1
ACGTTTA	295	0.0	18.813559	26
ACCGTTA	485	0.0	16.783504	8
TCACGTT	320	0.0	16.1875	24
GTATTGG	1855	0.0	15.857142	1
CGTGATT	70	0.0025939052	15.857142	12
GTATTAG	645	0.0	15.775193	1
CACGTTT	345	0.0	15.550724	25
TTAACGG	405	0.0	15.530864	35
TAATACT	770	0.0	15.376623	4
ATTAGAG	615	0.0	15.341463	3
ATTGGAC	1965	0.0	15.157762	3
GTCGTAC	75	0.004106621	14.8	1
GTATCAA	19375	0.0	14.723613	2
CTAATAC	705	0.0	14.695036	3
TTGGACC	2685	0.0	14.675978	4
CGTTATT	265	0.0	14.6603775	10
CCGTTTA	515	0.0	14.368932	27
TATACTG	440	0.0	13.875	5
CGTTTAC	560	0.0	13.875	28
>>END_MODULE