FastQCFastQC Report
Wed 25 May 2016
SRR2088589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3405679
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT375891.1037152943656756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT364271.069595813345885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT261160.7668368040558139No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147440.43292394849896304No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG81880.24042195403618485No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG78640.23090843265028793No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA75510.22171790118798632No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA75350.2212480976627568No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA72700.2134669767761436No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG62010.18207822874674917No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41490.12182592663606875No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA40320.11839048835782821No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40110.11777387123096451No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA38000.11157833724200078No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA37000.10864206520931656No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35570.10444319620257811No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC35510.10426701988061704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116650.024.5820831
GTATTGG15300.018.1372551
GTATCAA172400.016.5899092
ACGTTTA1251.6604099E-716.27999926
TTAACGG4050.015.98765535
ATTGGAC17800.015.9016873
TGGACCC22400.015.113845
TTGGACC23650.015.0972524
AGTACCG2850.014.9298245
CGCCTAA750.004106606414.81
AGTTCCG4250.014.79999932
CTAATAC9700.014.6855673
GGACCCT23200.014.5129316
ACCGTTA5200.013.8758
GTATTAG7850.013.668791
TAATACT9100.013.4175824
CGAACTA5800.013.39655124
CGCTACC5000.013.3228
TTCGCTA4900.013.21428526
TCGCTAC5200.013.16346227
Produced by FastQC (version 0.11.5)