##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088589_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3405679
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57833724200079	31.0	31.0	34.0	30.0	34.0
2	31.765818211287677	31.0	31.0	34.0	30.0	34.0
3	31.828238656667292	31.0	31.0	34.0	30.0	34.0
4	35.545785142992045	37.0	35.0	37.0	33.0	37.0
5	35.446800476498225	37.0	35.0	37.0	33.0	37.0
6	35.4838115982158	37.0	35.0	37.0	33.0	37.0
7	35.35371566139968	37.0	35.0	37.0	33.0	37.0
8	35.37986874276759	37.0	35.0	37.0	33.0	37.0
9	36.96143617763154	39.0	37.0	39.0	33.0	39.0
10	36.81435596249676	39.0	37.0	39.0	32.0	39.0
11	36.863677111084165	39.0	37.0	39.0	32.0	39.0
12	36.75945912694649	39.0	37.0	39.0	32.0	39.0
13	36.81015327633638	39.0	37.0	39.0	32.0	39.0
14	37.81705087296836	40.0	37.0	41.0	32.0	41.0
15	37.777127556648765	40.0	37.0	41.0	32.0	41.0
16	37.66335112616309	39.0	37.0	41.0	32.0	41.0
17	37.7044789012705	39.0	37.0	41.0	32.0	41.0
18	37.6804924950355	39.0	37.0	41.0	32.0	41.0
19	37.71225972852991	40.0	37.0	41.0	32.0	41.0
20	37.592035244660465	39.0	37.0	41.0	32.0	41.0
21	37.497598276290866	39.0	36.0	41.0	32.0	41.0
22	37.34330892606144	39.0	36.0	41.0	31.0	41.0
23	37.212337686552374	39.0	36.0	41.0	31.0	41.0
24	37.071214580117505	39.0	36.0	41.0	31.0	41.0
25	36.87707943115014	39.0	36.0	40.0	30.0	41.0
26	37.01729611040852	39.0	36.0	40.0	31.0	41.0
27	36.93864042970579	39.0	36.0	41.0	31.0	41.0
28	36.81169746179837	39.0	35.0	41.0	30.0	41.0
29	36.664623119207654	39.0	35.0	40.0	30.0	41.0
30	36.55509811699811	39.0	35.0	40.0	30.0	41.0
31	36.36860667138623	38.0	35.0	40.0	30.0	41.0
32	36.24893420665894	38.0	35.0	40.0	30.0	41.0
33	36.04781748367947	38.0	35.0	40.0	29.0	41.0
34	35.91223189267104	38.0	35.0	40.0	29.0	41.0
35	35.73052510233642	38.0	35.0	40.0	27.0	41.0
36	35.589668315774915	38.0	35.0	40.0	27.0	41.0
37	35.426802702192425	38.0	34.0	40.0	26.0	41.0
38	35.34088385898965	38.0	34.0	40.0	26.0	41.0
39	35.201793533683	38.0	34.0	40.0	25.0	41.0
40	35.01529327925503	38.0	34.0	40.0	24.0	41.0
41	34.88798004744428	38.0	34.0	40.0	24.0	41.0
42	34.67107880689871	38.0	33.0	40.0	23.0	41.0
43	34.30696081456884	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	9.0
15	16.0
16	51.0
17	118.0
18	283.0
19	582.0
20	1369.0
21	2680.0
22	4944.0
23	8144.0
24	13399.0
25	20720.0
26	29890.0
27	42021.0
28	56766.0
29	73938.0
30	93098.0
31	115040.0
32	140618.0
33	172629.0
34	213379.0
35	263003.0
36	340841.0
37	478528.0
38	658087.0
39	675522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.07001863651859	21.530890022224643	14.130221902886325	23.26886943837044
2	19.431837234219667	23.03928232813486	34.716131496832205	22.81274894081327
3	20.20554491483196	24.468953180848814	32.28454590112574	23.04095600319349
4	14.335496680691282	16.543162171185248	35.60450060032082	33.51684054780266
5	13.11568119015327	38.17467823596998	34.690439116546216	14.019201457330535
6	32.74850624501017	36.758719773648664	13.617431355098352	16.875342626242816
7	26.149792743238574	32.69465501593075	21.5512971128518	19.604255127978885
8	26.738045482266532	34.82078023207707	18.89388283511159	19.547291450544808
9	24.529998276408314	14.441202473867913	21.718840795036762	39.30995845468701
10	16.908170147568224	27.162131251947113	33.33954844246918	22.59015015801548
11	33.42293856819742	22.262315385566282	23.219980509026247	21.09476553721005
12	20.90593388278813	27.023950290088994	30.853671176878382	21.21644465024449
13	31.472960311291814	20.77485869924911	26.187435750697585	21.564745238761493
14	20.615184226111737	22.83042529845003	27.511753162878826	29.042637312559407
15	23.273303209139794	29.05253254930955	25.322997264275347	22.351166977275312
16	22.138463431227663	27.941917015667066	26.666870248194268	23.252749304911003
17	22.014875741371984	26.561722346703842	26.993912227194635	24.429489684729536
18	22.985431099055432	25.284884453291106	29.301264153198233	22.428420294455233
19	23.061245642939337	24.74440486023492	30.252293301864324	21.942056194961417
20	23.861115507362847	24.282881622137612	30.1841130652654	21.67188980523414
21	22.847749303442868	25.13833511613984	30.561101031541725	21.45281454887557
22	21.622560435085045	25.55701814528028	30.18226321388481	22.638158205749868
23	22.397883065315316	25.690207444682837	31.10378282862243	20.80812666137942
24	22.53183579544637	25.732812751877088	28.851280464189372	22.88407098848717
25	22.520296246357923	25.829709728955663	29.717069635746647	21.932924388939767
26	22.15793091480436	26.47260649051188	29.783811099049558	21.585651495634202
27	21.349399047884432	26.5255768379815	29.438769772488833	22.686254341645235
28	21.607145006913452	26.24381217372512	29.659430615745052	22.48961220361637
29	22.001926781707848	26.116789045591204	29.192798264310877	22.688485908390074
30	21.952098245313195	26.21101401512004	30.559456719203425	21.27743102036334
31	22.385903075421965	25.940407184587862	30.446468971385737	21.22722076860444
32	22.073425005703708	25.74678940675266	29.534609691635648	22.645175895907983
33	21.723480104848402	25.903145892493097	30.083105307341064	22.29026869531744
34	21.212744947483305	26.199680005073876	30.5494733942923	22.038101653150516
35	20.889373308523794	27.847545232536596	29.60516830858105	21.657913150358564
36	22.693771198048907	25.831530217615928	29.488715759764794	21.98598282457037
37	21.7537824322257	26.296782521194746	29.595331797271555	22.354103249307993
38	21.66722113270217	25.113817244666926	30.77442119471624	22.44454042791467
39	21.138721529539335	25.038355053426937	31.421252560796248	22.40167085623748
40	20.311074531686632	25.71704497106157	30.962254516647047	23.00962598060475
41	21.31871500514288	24.698863281007988	31.1443327453938	22.838088968455335
42	20.24759233034	26.528248845531245	30.52797988301305	22.696178941115708
43	20.612776483044936	26.273615334856864	29.77485546934987	23.338752712748324
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	266.0
1	472.0
2	678.0
3	1575.0
4	2472.0
5	2472.0
6	3988.5
7	5505.0
8	6143.0
9	6781.0
10	10181.5
11	13582.0
12	13582.0
13	25535.0
14	37488.0
15	57847.0
16	78206.0
17	71577.5
18	64949.0
19	64949.0
20	71597.0
21	78245.0
22	62090.5
23	45936.0
24	51199.5
25	56463.0
26	56463.0
27	61884.5
28	67306.0
29	72910.0
30	78514.0
31	87142.5
32	95771.0
33	95771.0
34	106574.0
35	117377.0
36	130067.5
37	142758.0
38	153882.0
39	165006.0
40	165006.0
41	172415.0
42	179824.0
43	194018.0
44	208212.0
45	219554.5
46	230897.0
47	230897.0
48	289766.0
49	348635.0
50	331805.0
51	314975.0
52	291006.0
53	267037.0
54	267037.0
55	238944.5
56	210852.0
57	183133.0
58	155414.0
59	139279.5
60	123145.0
61	123145.0
62	108830.0
63	94515.0
64	82055.5
65	69596.0
66	59391.5
67	49187.0
68	49187.0
69	42342.5
70	35498.0
71	30288.0
72	25078.0
73	20389.0
74	15700.0
75	15700.0
76	12437.5
77	9175.0
78	7088.0
79	5001.0
80	3873.0
81	2745.0
82	2745.0
83	2080.0
84	1415.0
85	1074.5
86	734.0
87	557.5
88	381.0
89	381.0
90	303.0
91	225.0
92	148.0
93	71.0
94	63.0
95	55.0
96	55.0
97	30.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3405679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.226447763954184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.03866876978172	36.074504454548375
2	12.035020528128232	11.12672495563272
3	4.022517612739239	5.578401009146284
4	1.7887923822755538	3.307580704792802
5	0.9595208736159538	2.217762077131579
6	0.602801776235471	1.6719230892700683
7	0.4099559102813876	1.32655638305028
8	0.2928694152343275	1.0830650179991559
9	0.21863216313578537	0.9095929441845023
>10	1.3736215447215225	12.190649783095646
>50	0.14658805698508462	4.700797450492039
>100	0.09788681849087338	8.720720408255945
>500	0.008091101182834789	2.562115534530702
>1k	0.004395952610784918	3.832674155744046
>5k	3.822567487639059E-4	1.3137626800081923
>10k+	2.548378325092706E-4	3.383169352117759
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37589	1.1037152943656756	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36427	1.069595813345885	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26116	0.7668368040558139	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14744	0.43292394849896304	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8188	0.24042195403618485	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7864	0.23090843265028793	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7551	0.22171790118798632	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7535	0.2212480976627568	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7270	0.2134669767761436	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6201	0.18207822874674917	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4149	0.12182592663606875	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4032	0.11839048835782821	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4011	0.11777387123096451	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3800	0.11157833724200078	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3700	0.10864206520931656	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3557	0.10444319620257811	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3551	0.10426701988061704	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	2.9362720326842314E-5	0.0
7	0.0	0.0	2.9362720326842314E-5	2.9362720326842314E-5	0.0
8	2.9362720326842314E-5	0.0	2.9362720326842314E-5	2.9362720326842314E-5	0.0
9	8.808816098052694E-5	0.0	2.9362720326842314E-5	1.7617632196105388E-4	0.0
10	1.1745088130736925E-4	0.0	2.9362720326842314E-5	3.2298992359526546E-4	0.0
11	1.4681360163421156E-4	0.0	2.9362720326842314E-5	5.578916862100039E-4	0.0
12	1.4681360163421156E-4	0.0	2.9362720326842314E-5	8.808816098052693E-4	0.0
13	1.4681360163421156E-4	0.0	2.9362720326842314E-5	9.102443301321117E-4	0.0
14	1.4681360163421156E-4	0.0	2.9362720326842314E-5	0.0011745088130736925	0.0
15	1.4681360163421156E-4	0.0	2.9362720326842314E-5	0.0015855868976494848	0.0
16	1.4681360163421156E-4	0.0	2.9362720326842314E-5	0.0022315667448400156	0.0
17	1.4681360163421156E-4	0.0	2.9362720326842314E-5	0.0031418110749721275	0.0
18	1.7617632196105388E-4	0.0	2.9362720326842314E-5	0.0036409773205284468	0.0
19	1.7617632196105388E-4	0.0	2.9362720326842314E-5	0.004844848853928982	0.0
20	1.7617632196105388E-4	0.0	2.9362720326842314E-5	0.007957297208574266	0.0
21	1.7617632196105388E-4	0.0	2.9362720326842314E-5	0.012743420621849563	0.0
22	2.055390422878962E-4	0.0	2.9362720326842314E-5	0.021816501202843838	0.0
23	2.055390422878962E-4	0.0	2.9362720326842314E-5	0.027072428141348612	0.0
24	2.055390422878962E-4	0.0	2.9362720326842314E-5	0.03517653895155709	0.0
25	2.055390422878962E-4	0.0	2.9362720326842314E-5	0.040872906694964496	0.0
26	2.055390422878962E-4	0.0	2.9362720326842314E-5	0.05167838777524247	0.0
27	2.055390422878962E-4	0.0	2.9362720326842314E-5	0.10438447076192442	0.0
28	2.055390422878962E-4	0.0	2.9362720326842314E-5	0.2226868709587721	0.0
29	2.349017626147385E-4	0.0	2.9362720326842314E-5	0.3738167924810295	0.0
30	2.642644829415808E-4	0.0	2.9362720326842314E-5	0.5914532755435847	0.0
31	2.642644829415808E-4	0.0	2.9362720326842314E-5	0.9003784561022927	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11665	0.0	24.582083	1
GTATTGG	1530	0.0	18.137255	1
GTATCAA	17240	0.0	16.589909	2
ACGTTTA	125	1.6604099E-7	16.279999	26
TTAACGG	405	0.0	15.987655	35
ATTGGAC	1780	0.0	15.901687	3
TGGACCC	2240	0.0	15.11384	5
TTGGACC	2365	0.0	15.097252	4
AGTACCG	285	0.0	14.929824	5
CGCCTAA	75	0.0041066064	14.8	1
AGTTCCG	425	0.0	14.799999	32
CTAATAC	970	0.0	14.685567	3
GGACCCT	2320	0.0	14.512931	6
ACCGTTA	520	0.0	13.875	8
GTATTAG	785	0.0	13.66879	1
TAATACT	910	0.0	13.417582	4
CGAACTA	580	0.0	13.396551	24
CGCTACC	500	0.0	13.32	28
TTCGCTA	490	0.0	13.214285	26
TCGCTAC	520	0.0	13.163462	27
>>END_MODULE