FastQCFastQC Report
Wed 25 May 2016
SRR2088588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3712071
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT368350.9923032183382268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT356090.9592758328167753No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT264630.7128904592611509No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139390.3755046711121635No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG81890.22060461666816178No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG78520.21152612652074812No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA77960.21001753468616308No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA76420.20586890714105413No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA74950.20190885357526836No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG62440.16820798955623423No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA42410.11424889232991503No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA41050.1105851693030656No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40830.10999250822519288No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA39380.10608633293921373No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39160.10549367186134101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109750.024.0879271
GTATTGG16700.018.2784421
GTATCAA167400.015.78136352
GTACCGA855.368153E-415.2352946
TTGGACC24050.015.0769244
TGGACCC23850.014.970655
ATTGGAC19800.014.9494953
GGACCCT24100.014.50836
GTATACG2800.013.8750011
TTCGTTA3750.013.81333430
CTAATAC9850.013.710663
ATACTGG9450.013.7037046
TAATACT9950.013.5728654
TATACCG2051.4260877E-913.5365865
TATACTG5150.013.2912635
CGAACTA7250.013.01379324
CGAACGT3700.013.012
TACCCCG4000.012.9500015
TACTCCG10050.012.88557155
ACCGTTA5750.012.8695648
Produced by FastQC (version 0.11.5)