FastQCFastQC Report
Wed 25 May 2016
SRR2088587_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088587_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045162
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT151121.445900252783779No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT149781.433079273835061No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT97350.9314345527296246No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58310.55790394216399No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA32320.3092343579272878No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG31180.29832695792613967No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA30990.29650905792594834No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG30650.29325597371507955No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA28320.27096277897589083No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG23570.22551527897110685No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17890.1711696368601231No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16900.16169742106965235No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA15650.1497375526473408No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA14100.13490731580367446No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA13750.1315585526454272No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13590.13002768948737134No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC13310.12734867896077354No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA13140.12572213685533917No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC12720.12170362106544248No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG12690.121416584223307No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG12150.11624992106486841No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG11570.11070054211691585No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC11430.10936103685361695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTACG250.00549611829.616
GGTATCA48350.021.8862461
TATACCG450.003825580220.5555555
CTGTGCG1052.258821E-819.380959
GTATTAG1850.019.01
TGTGCGC1306.9667294E-1018.510
AGTTCCG752.06704E-417.26666632
TAGGTCG650.001579804717.07692321
AATGCGA650.001579804717.07692320
TAAGCCG650.001579804717.07692310
GTATTGG5800.016.9051741
TTAACGG1005.8767637E-616.65000235
ATAGACT803.382198E-416.18754
CCTATAG803.382198E-416.18752
TAATACT2300.016.0869564
ATTAGAG1504.667527E-916.0333333
ATTGGAC6000.016.0333333
GTGCGCC1851.8189894E-1116.011
ATACACA4450.015.79775337
TTGGACC8100.015.7592594
Produced by FastQC (version 0.11.5)