##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088587_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1045162
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32888968408725	31.0	31.0	33.0	30.0	34.0
2	31.517627889265015	31.0	31.0	34.0	30.0	34.0
3	31.551018885110633	31.0	31.0	34.0	30.0	34.0
4	35.31135173303277	37.0	35.0	37.0	33.0	37.0
5	35.20996170928526	37.0	35.0	37.0	33.0	37.0
6	35.27610169523959	37.0	35.0	37.0	32.0	37.0
7	35.130731886540076	37.0	35.0	37.0	32.0	37.0
8	35.176897935439676	37.0	35.0	37.0	32.0	37.0
9	36.70516053970581	39.0	35.0	39.0	32.0	39.0
10	36.53497448242474	38.0	35.0	39.0	32.0	39.0
11	36.608022488379795	38.0	35.0	39.0	32.0	39.0
12	36.47674523183966	38.0	35.0	39.0	32.0	39.0
13	36.54977984274208	38.0	35.0	39.0	32.0	39.0
14	37.43705760446706	39.0	36.0	41.0	32.0	41.0
15	37.411779226569664	39.0	36.0	41.0	32.0	41.0
16	37.266727071975446	39.0	36.0	40.0	32.0	41.0
17	37.34980031803682	39.0	36.0	40.0	32.0	41.0
18	37.338428875140885	39.0	36.0	40.0	32.0	41.0
19	37.37340431435509	39.0	36.0	40.0	32.0	41.0
20	37.25809970129033	39.0	36.0	40.0	31.0	41.0
21	37.16500599907	39.0	36.0	40.0	31.0	41.0
22	36.99772953857871	39.0	36.0	40.0	31.0	41.0
23	36.856595436879644	39.0	35.0	40.0	31.0	41.0
24	36.702793442547666	39.0	35.0	40.0	30.0	41.0
25	36.49623790378908	38.0	35.0	40.0	30.0	41.0
26	36.60116230785275	38.0	35.0	40.0	30.0	41.0
27	36.50453900926364	38.0	35.0	40.0	30.0	41.0
28	36.357332164774455	38.0	35.0	40.0	30.0	41.0
29	36.187325983914455	38.0	35.0	40.0	30.0	41.0
30	36.04864891758407	38.0	35.0	40.0	29.0	41.0
31	35.813037596085586	38.0	35.0	40.0	29.0	41.0
32	35.67560148570269	38.0	34.0	40.0	28.0	41.0
33	35.44890552852094	38.0	34.0	40.0	27.0	41.0
34	35.27178848829177	38.0	34.0	40.0	27.0	41.0
35	35.068764459480924	38.0	34.0	40.0	25.0	41.0
36	34.90510849035843	38.0	33.0	40.0	25.0	41.0
37	34.714979113285786	38.0	33.0	40.0	24.0	41.0
38	34.59414042990465	38.0	33.0	40.0	24.0	41.0
39	34.3969049774102	38.0	33.0	40.0	23.0	41.0
40	34.163333531069824	38.0	33.0	40.0	22.0	41.0
41	33.99521413905213	37.0	33.0	40.0	21.0	41.0
42	33.71476670602261	37.0	33.0	40.0	19.0	41.0
43	33.329906751297884	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	7.0
15	6.0
16	27.0
17	48.0
18	116.0
19	280.0
20	533.0
21	1119.0
22	1904.0
23	3314.0
24	5454.0
25	7981.0
26	11373.0
27	15602.0
28	21063.0
29	26777.0
30	33086.0
31	40441.0
32	48853.0
33	59401.0
34	72014.0
35	87866.0
36	113457.0
37	155155.0
38	196924.0
39	142358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.3856052937248	22.546361233952247	15.046853980531246	22.021179491791703
2	20.030578991582164	24.057610207795538	33.97281952462872	21.938991275993576
3	21.195278818020554	24.77807268155559	32.772718487660285	21.253930012763572
4	15.102826164747665	17.16078464391166	34.61693019838073	33.11945899295994
5	12.943735038204604	38.78747983566184	34.31334089834877	13.955444227784783
6	32.5875797244829	36.49539497226267	12.989756611893657	17.927268691360766
7	25.37137783424962	33.524946371949994	21.182075123282324	19.921600670518064
8	27.039540281793634	34.994575003683636	18.4378115545724	19.528073159950324
9	24.09884783411567	14.607687612063966	22.213972570759367	39.079491983061
10	17.32726601235024	27.64078678712008	32.77558885608164	22.25635834444804
11	32.92570912451849	22.28869782866197	23.224437934023626	21.56115511279591
12	20.85179139693177	27.68977440817787	30.808238340085076	20.650195854805283
13	32.40502429288474	20.537773091635554	26.5837257764825	20.47347683899721
14	20.138026449488216	23.65700245512179	28.05057971874217	28.15439137664783
15	23.030975102424314	29.387693008356596	25.803655318505648	21.77767657071344
16	21.378025607513475	28.138987066119892	27.581465839745416	22.901521486621213
17	21.60755940227448	26.719876918602093	27.480620229208487	24.19194344991494
18	22.93979306557261	24.84944917629994	29.954208055784655	22.256549702342795
19	22.72346296554984	24.42807909204506	31.568503255954578	21.279954686450523
20	23.653462334068788	23.94824917094192	31.36604660330169	21.032241891687605
21	22.730351849761092	24.776350460502776	31.881851808619142	20.611445881116992
22	20.922115423254958	25.388025971093477	31.353512661195104	22.336345944456458
23	22.00280913389503	25.62597951322379	32.527684703423965	19.843526649457214
24	22.043281328636137	25.58694250269336	29.95057225578427	22.419203912886232
25	21.952577686521323	25.90698858167442	30.705383471653196	21.43505026015106
26	21.603636565431962	26.69203434491495	31.003614750631957	20.70071433902113
27	20.69679150217861	26.609176376485177	30.678019292702952	22.016012828633265
28	21.154902302226834	26.48249745015605	30.63544216111952	21.727158086497596
29	21.35955957066943	26.145229160646867	29.979467297892576	22.51574397079113
30	21.324062681192007	26.399161086989388	31.875728355986922	20.40104787583169
31	22.0060622181059	25.86001021851158	31.646290240173293	20.487637323209228
32	21.63616740754065	25.73744548691973	30.500726203210604	22.125660902329017
33	21.186954749598627	26.104182892221495	31.002466603263418	21.706395754916464
34	20.53806012847769	26.677012750176527	31.46995394015473	21.31497318119105
35	20.07353883895511	28.729421850392573	30.47431881373414	20.722720496918182
36	22.27463302339733	26.15192668696336	30.449346608468353	21.124093681170958
37	21.24015224434107	27.002416850210782	30.188812834756718	21.568618070691432
38	21.283016412766635	25.41271113951713	31.634904445435254	21.669368002280986
39	20.61355081795932	25.222405713181306	32.39048109288321	21.773562375976162
40	19.533718217845657	26.157284708016554	31.770481513870575	22.53851556026721
41	21.05233447063709	25.077165071060755	31.7272346296555	22.143265828646662
42	19.807168649453384	27.104984681800527	30.93319504536139	22.154651623384698
43	20.167495565280788	27.095990860746948	29.97975433473471	22.756759239237553
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	58.0
1	131.0
2	204.0
3	431.5
4	659.0
5	659.0
6	1245.0
7	1831.0
8	2093.0
9	2355.0
10	3723.5
11	5092.0
12	5092.0
13	9954.5
14	14817.0
15	23047.5
16	31278.0
17	28000.5
18	24723.0
19	24723.0
20	27586.0
21	30449.0
22	23282.0
23	16115.0
24	17247.5
25	18380.0
26	18380.0
27	19872.5
28	21365.0
29	22894.0
30	24423.0
31	26169.0
32	27915.0
33	27915.0
34	30916.5
35	33918.0
36	37250.5
37	40583.0
38	43324.5
39	46066.0
40	46066.0
41	48287.0
42	50508.0
43	54352.0
44	58196.0
45	63644.0
46	69092.0
47	69092.0
48	92722.0
49	116352.0
50	108836.0
51	101320.0
52	92750.0
53	84180.0
54	84180.0
55	73959.0
56	63738.0
57	54253.0
58	44768.0
59	38895.0
60	33022.0
61	33022.0
62	29237.0
63	25452.0
64	22089.0
65	18726.0
66	15933.5
67	13141.0
68	13141.0
69	11456.5
70	9772.0
71	8143.0
72	6514.0
73	5394.5
74	4275.0
75	4275.0
76	3463.0
77	2651.0
78	2109.5
79	1568.0
80	1192.5
81	817.0
82	817.0
83	606.5
84	396.0
85	301.5
86	207.0
87	159.0
88	111.0
89	111.0
90	89.5
91	68.0
92	51.5
93	35.0
94	24.5
95	14.0
96	14.0
97	10.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1045162.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.37610263996741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.65973884438954	46.03466648875361
2	8.762705358838682	9.529635319920091
3	2.4354727751097593	3.972945527886435
4	1.0985814646162813	2.389463139133625
5	0.6353412481193266	1.7273690459570747
6	0.41586723376408	1.356794363265296
7	0.299101564504527	1.1384784159891095
8	0.21194061704249892	0.9219603796704756
9	0.17104794478282045	0.837082854167951
>10	1.124513345058569	11.705718928961744
>50	0.11473697154771349	4.344904571797283
>100	0.060539186514402946	5.887867381196156
>500	0.006177467795152537	2.2565247192159537
>1k	0.0035299815972300213	3.5148309285405697
>5k	3.529981597230021E-4	1.4939207119477513
>10k+	3.529981597230021E-4	2.887837223596803
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15112	1.445900252783779	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14978	1.433079273835061	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9735	0.9314345527296246	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5831	0.55790394216399	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3232	0.3092343579272878	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3118	0.29832695792613967	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3099	0.29650905792594834	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3065	0.29325597371507955	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2832	0.27096277897589083	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2357	0.22551527897110685	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1789	0.1711696368601231	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1690	0.16169742106965235	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1565	0.1497375526473408	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1410	0.13490731580367446	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1375	0.1315585526454272	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1359	0.13002768948737134	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1331	0.12734867896077354	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1314	0.12572213685533917	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1272	0.12170362106544248	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1269	0.121416584223307	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1215	0.11624992106486841	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1157	0.11070054211691585	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1143	0.10936103685361695	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	9.567894737849252E-5	0.0	0.0	0.0	0.0
7	1.9135789475698505E-4	0.0	0.0	0.0	0.0
8	1.9135789475698505E-4	0.0	0.0	0.0	0.0
9	2.8703684213547757E-4	0.0	0.0	1.9135789475698505E-4	0.0
10	3.827157895139701E-4	0.0	0.0	4.783947368924626E-4	0.0
11	5.740736842709551E-4	0.0	0.0	0.0010524684211634177	0.0
12	5.740736842709551E-4	0.0	0.0	0.002009257894948343	0.0
13	5.740736842709551E-4	0.0	0.0	0.002200615789705328	0.0
14	5.740736842709551E-4	0.0	0.0	0.002774689473976283	0.0
15	6.697526316494476E-4	0.0	0.0	0.0034444421056257306	0.0
16	6.697526316494476E-4	0.0	0.0	0.004209873684653671	0.0
17	6.697526316494476E-4	0.0	0.0	0.0054537000005740735	0.0
18	6.697526316494476E-4	0.0	0.0	0.006027773684845029	0.0
19	6.697526316494476E-4	0.0	0.0	0.007462957895522417	0.0
20	7.654315790279402E-4	0.0	0.0	0.01176851052755458	0.0
21	7.654315790279402E-4	0.0	0.0	0.01894443158094152	0.0
22	8.611105264064327E-4	0.0	0.0	0.031956768424416505	0.0
23	8.611105264064327E-4	0.0	0.0	0.03951540526731741	0.0
24	8.611105264064327E-4	0.0	0.0	0.053771568426712796	0.0
25	8.611105264064327E-4	0.0	0.0	0.061425884216992195	0.0
26	8.611105264064327E-4	0.0	0.0	0.07348143158668226	0.0
27	8.611105264064327E-4	0.0	0.0	0.12399991580252631	0.0
28	8.611105264064327E-4	0.0	0.0	0.24599057371010427	0.0
29	8.611105264064327E-4	0.0	0.0	0.40510466320053734	0.0
30	8.611105264064327E-4	0.0	0.0	0.626697105329126	0.0
31	9.567894737849252E-4	0.0	0.0	0.9397586211515535	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTACG	25	0.005496118	29.6	16
GGTATCA	4835	0.0	21.886246	1
TATACCG	45	0.0038255802	20.555555	5
CTGTGCG	105	2.258821E-8	19.38095	9
GTATTAG	185	0.0	19.0	1
TGTGCGC	130	6.9667294E-10	18.5	10
AGTTCCG	75	2.06704E-4	17.266666	32
TAGGTCG	65	0.0015798047	17.076923	21
AATGCGA	65	0.0015798047	17.076923	20
TAAGCCG	65	0.0015798047	17.076923	10
GTATTGG	580	0.0	16.905174	1
TTAACGG	100	5.8767637E-6	16.650002	35
ATAGACT	80	3.382198E-4	16.1875	4
CCTATAG	80	3.382198E-4	16.1875	2
TAATACT	230	0.0	16.086956	4
ATTAGAG	150	4.667527E-9	16.033333	3
ATTGGAC	600	0.0	16.033333	3
GTGCGCC	185	1.8189894E-11	16.0	11
ATACACA	445	0.0	15.797753	37
TTGGACC	810	0.0	15.759259	4
>>END_MODULE